Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1978 Jun 15;172(3):577–585. doi: 10.1042/bj1720577

Effects of hormones and N6O2'-dibutyryl-adenosine 3' :5'-cyclic monophosphate, administered in vivo, on phosphate transport and metabolism in isolated rat liver mitochondria.

G J Barritt, R F Thorne, B P Hughes
PMCID: PMC1185733  PMID: 210763

Abstract

1. The administration of glucagon or N6O2'-dibutyryl cyclic AMP to fed rats by intraperitoneal injection was associated with a 2-fold increase in the amounts of endogenous Pi and ATP, and an increase in the rate and extent of transport of exogenous Pi (measured in either the presence or the absence of Ca2+) in mitochondria subsequently isolated from the liver. No change was observed in either the maximum rate of transport of exogenous Pi or in the rate of 32Pi exchange. 2. The changes induced by glucagon and dibutyryl cyclic AMP were markedly decreased by the co-administration of cycloheximide. 3. The administration of insulin to rats resulted in an increase of about 1.3-fold in the concentration of endogenous mitochondrial Pi 4. The amounts of endogenous Pi in mitochondrial isolated from the livers of starved rats were 3 times those in mitochondria isolated from fed animals. 5. It is concluded that the liver mitochondrial phosphatetransport system may be an important site of hormone action. 6. In the course of these experiments, it was shown that Ca2+ markedly stimulates mitochondrial phosphate transports.

Full text

PDF
577

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barritt G. J., Dorman D. M., Bygrave F. L. Administration of insulin to rats induces cycloheximide-sensitive changes in the calcium-ion-transport system of mitochondria isolated subsequently from liver. Biochem Soc Trans. 1975;3(5):711–712. doi: 10.1042/bst0030711. [DOI] [PubMed] [Google Scholar]
  2. Biebuyck J. F. Anesthesia and hepatic metabolism: current concepts of carbohydrate homeostasis. Anesthesiology. 1973 Aug;39(2):188–198. doi: 10.1097/00000542-197308000-00010. [DOI] [PubMed] [Google Scholar]
  3. Coty W. A., Pedersen P. L. Phosphate transport in rat liver mitochondria. Kinetics and energy requirements. J Biol Chem. 1974 Apr 25;249(8):2593–2598. [PubMed] [Google Scholar]
  4. Coty W. A., Pedersen P. L. Phosphate transport in rat liver mitochondria. Kinetics, inhibitor sensitivity, energy requirements, and labeled components. Mol Cell Biochem. 1975 Nov 14;9(2):109–124. doi: 10.1007/BF01732202. [DOI] [PubMed] [Google Scholar]
  5. Dorman D. M., Barritt G. J., Bygrave F. L. Stimulation of hepatic mitochondrial calcium transport by elevated plasma insulin concentrations. Biochem J. 1975 Sep;150(3):389–395. doi: 10.1042/bj1500389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Foldes M., Barritt G. J. Regulation by calcium ions of pyruvate carboxylation, pyruvate transport, and adenine nucleotide transport in isolated rat liver mitochondria. J Biol Chem. 1977 Aug 10;252(15):5372–5380. [PubMed] [Google Scholar]
  7. Halestrap A. P. The mechanism of the stimulation of pyruvate transport into rat liver mitochondria by glucagon. Biochem Soc Trans. 1977;5(1):216–219. doi: 10.1042/bst0050216. [DOI] [PubMed] [Google Scholar]
  8. Hems D. A., Whitton P. D., Ma G. Y. Metabolic actions of vasopressin, glucagon and adrenalin in the intact rat. Biochim Biophys Acta. 1975 Nov 10;411(1):155–164. doi: 10.1016/0304-4165(75)90294-9. [DOI] [PubMed] [Google Scholar]
  9. Lehninger A. L. Role of phosphate and other proton-donating anions in respiration-coupled transport of Ca2+ by mitochondria. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1520–1524. doi: 10.1073/pnas.71.4.1520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McGivan J. D., Klingenberg M. Correlation between H+ and anion movement in mitochondria and the key role of the phosphate carrier. Eur J Biochem. 1971 Jun 11;20(3):392–399. doi: 10.1111/j.1432-1033.1971.tb01405.x. [DOI] [PubMed] [Google Scholar]
  11. Reed K. C., Bygrave F. L. A kinetic study of mitochondrial calcium transport. Eur J Biochem. 1975 Jul 15;55(3):497–504. doi: 10.1111/j.1432-1033.1975.tb02187.x. [DOI] [PubMed] [Google Scholar]
  12. Reed K. C., Bygrave F. L. Accumulation of lanthanum by rat liver mitochondria. Biochem J. 1974 Feb;138(2):239–252. doi: 10.1042/bj1380239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Titheradge M. A., Coore H. G. Hormonal regulation of liver mitochondrial pyruvate carrier in relation to gluconeogenesis and lipogenesis. FEBS Lett. 1976 Nov 15;72(1):73–78. doi: 10.1016/0014-5793(76)80901-5. [DOI] [PubMed] [Google Scholar]
  14. Wititsuwannakul D., Kim K. H. Effect of cycloheximide on adenosine 3':5'-monophosphate level in rat epididymal fat tissue. Biochem Biophys Res Commun. 1977 May 9;76(1):86–92. doi: 10.1016/0006-291x(77)91671-0. [DOI] [PubMed] [Google Scholar]
  15. Yamazaki R. K. Glucagon stimulation of mitochondrial respiration. J Biol Chem. 1975 Oct 10;250(19):7924–7930. [PubMed] [Google Scholar]
  16. Yamazaki R. K., Sax R. D., Hauser M. A. Glucagon stimulation of mitochondrial ATPase and potassium ion transport. FEBS Lett. 1977 Mar 15;75(1):295–299. doi: 10.1016/0014-5793(77)80106-3. [DOI] [PubMed] [Google Scholar]
  17. Zahlten R. N., Hochberg A. A., Stratman F. W., Lardy H. A. Glucagon-stimulated phosphorylation of mitochondrial and lysosomal membranes of rat liver in vivo. Proc Natl Acad Sci U S A. 1972 Apr;69(4):800–804. doi: 10.1073/pnas.69.4.800. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES