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. 1978 Jul 1;173(1):333–336. doi: 10.1042/bj1730333

Blood-group-I activity associated with band 3, the major intrinsic membrane protein of human erythrocytes.

R A Childs, T Feizi, M Fukuda, S I Hakomori
PMCID: PMC1185778  PMID: 687374

Abstract

Affinity chromatography of radioiodinated solubilized erythrocyte stroma and of radioiodinated purified Band 3 on an anti-(blood-group I)-adsorbent column showed blood-group-I activity associated with a subpopulation of Band 3. The specificity of binding was confirmed by inhibition with known blood-group substances in radioimmunoassays.

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Selected References

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  1. Bretscher M. S. A major protein which spans the human erythrocyte membrane. J Mol Biol. 1971 Jul 28;59(2):351–357. doi: 10.1016/0022-2836(71)90055-6. [DOI] [PubMed] [Google Scholar]
  2. Butters T. D., Hughes R. C. Surface labelling for human tumour KB cells. Iodination and fractionation of membrane glycoproteins. Biochem J. 1975 Jul;150(1):59–69. doi: 10.1042/bj1500059. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cabantchik Z. I., Rothstein A. Membrane proteins related to anion permeability of human red blood cells. II. Effects of proteolytic enzymes on disulfonic stilbene sites of surface proteins. J Membr Biol. 1974;15(3):227–248. doi: 10.1007/BF01870089. [DOI] [PubMed] [Google Scholar]
  4. DODGE J. T., MITCHELL C., HANAHAN D. J. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch Biochem Biophys. 1963 Jan;100:119–130. doi: 10.1016/0003-9861(63)90042-0. [DOI] [PubMed] [Google Scholar]
  5. Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
  6. Feizi T., Cederqvist L. L., Childs R. The blood group I and i antigens of amniotic fluid. I. Association of I and i antigens with blood group A, B and H antigens. Br J Haematol. 1975 Aug;30(4):489–497. doi: 10.1111/j.1365-2141.1975.tb01863.x. [DOI] [PubMed] [Google Scholar]
  7. Feizi T., Kabat E. A., Vicari G., Anderson B., Marsh W. L. Immunochemical studies on blood groups.XLIX. The I antigen complex: specificity differences among anti-I sera revealed by quantitative precipitin studies; partial structure of the I determinant specific for one anti-I serum. J Immunol. 1971 Jun;106(6):1578–1592. [PubMed] [Google Scholar]
  8. Findlay J. B. The receptor proteins for concanavalin A and Lens culinaris phytohemagglutinin in the membrane of the human erythrocyte. J Biol Chem. 1974 Jul 25;249(14):4398–4403. [PubMed] [Google Scholar]
  9. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gardas A. Studies on the I-blood-group-active sites on macro-glycolipids from human erythrocytes. Eur J Biochem. 1976 Sep;68(1):185–191. doi: 10.1111/j.1432-1033.1976.tb10777.x. [DOI] [PubMed] [Google Scholar]
  11. Jenkins R. E., Tanner J. A. The major human erythrocyte membrane protein. Evidence for an S-shaped structure which traverses the membrane twice and contains a duplicated set of sites. Biochem J. 1975 Jun;147(3):393–399. doi: 10.1042/bj1470393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kościelak J., Miller-Podraza H., Krauze R., Piasek A. Isolation and characterization of poly(glycosyl)ceramides (megaloglycolipids) with A, H and I blood-group activities. Eur J Biochem. 1976 Dec;71(1):9–18. doi: 10.1111/j.1432-1033.1976.tb11083.x. [DOI] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Lecomte J., Feizi T. A common idiotype on human macroglobulins with anti-I and anti-i specificity. Clin Exp Immunol. 1975 May;20(2):287–302. [PMC free article] [PubMed] [Google Scholar]
  16. Marchesi V. T., Furthmayr H., Tomita M. The red cell membrane. Annu Rev Biochem. 1976;45:667–698. doi: 10.1146/annurev.bi.45.070176.003315. [DOI] [PubMed] [Google Scholar]
  17. Steck T. L., Ramos B., Strapazon E. Proteolytic dissection of band 3, the predominant transmembrane polypeptide of the human erythrocyte membrane. Biochemistry. 1976 Mar 9;15(5):1153–1161. doi: 10.1021/bi00650a030. [DOI] [PubMed] [Google Scholar]
  18. Watanabe K., Laine R. A., Hakomori S. I. On neutral fucoglycolipids having long, branched carbohydrate chains: H-active and I-active glycosphingolipids of human erythrocyte membranes. Biochemistry. 1975 Jun 17;14(12):2725–2733. doi: 10.1021/bi00683a026. [DOI] [PubMed] [Google Scholar]

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