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. 1978 Sep 1;173(3):767–771. doi: 10.1042/bj1730767

Isolation of human lactate dehydrogenase isoenzyme X by affinity chromatography.

A H Kolk, L van Kuyk, B Boettcher
PMCID: PMC1185842  PMID: 213050

Abstract

Human isoenzyme LDH-X (lactate dehydrogenase isoenzyme X) was isolated from seminal fluid of frozen semen samples by affinity chromatography by using oxamate-Sepharose and AMP-Sepharose. In the presence of 1.6 mM-NAD+, isoenzyme LDH-X does not bind to AMP-Sepharose, whereas the other lactate dehydrogenase isoenzymes do. This is the crucial point in the isolation of isoenzyme LDH-X from the other isoenzymes. The purified human isoenzyme LDH-X had a specific activity of 146 units/mg of protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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