Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1978 Aug 15;174(2):453–459. doi: 10.1042/bj1740453

The pH-dependence of sugar-transport and glycolysis in cultured Ehrlich ascites-tumour cells.

E Kaminskas
PMCID: PMC1185934  PMID: 30454

Abstract

1. pH-dependence of glycolysis has generally been ascribed to the effects of pH on the activities of glycolytic enzymes. The present study shows that sugar transport is pH-dependent in cultured Ehrlich ascites-tumour cells. 2. The rates of glucose consumption, of 3-O-methylglucose transport, and of 2-deoxyglucose transport and phosphorylation increased as linear functions of pH, as the pH of the cell culture medium was increased from 6.1 to 8.5. Transport of glucose, as measured in ATP-depleted cells, was pH-dependent to the same extent as transport of the non-metabolizable sugars. 3. Glucose consumption rates were about 8-fold higher at pH 8.5 than at pH 6.4. About 65-85% of glucose was converted into lactate. Sugar transport rates were 2.5-fold higher at pH 8.5 than at pH 6.3. 4. pH affected both simple diffusion and facilitated diffusion. pH effect was mainly on the Vmax. of 2-deoxyglucose uptake, and on the rapid-uptake phase of 3-O-methylglucose transport. 5. It was estimated that about 70% of the pH effect on the rates of glucose consumption may be due to the effect on sugar transport and the remainder to the effect on the activities of glycolytic enzymes.

Full text

PDF
453

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Christopher C. W., Kohlbacher M. S., Amos H. Transport of sugars in chick-embryo fibroblasts. Evidence for a low-affinity system and a high-affinity system for glucose transport. Biochem J. 1976 Aug 15;158(2):439–450. doi: 10.1042/bj1580439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Delcher H. K., Shipp J. C. Effect of pH, pCO2 and bicarbonate on metabolism of glucose by perfused rat heart. Biochim Biophys Acta. 1966 Jun 29;121(2):250–260. doi: 10.1016/0304-4165(66)90114-0. [DOI] [PubMed] [Google Scholar]
  3. Fodge D. W., Rubin H. Activation of phosphofructokinase by stimulants of cell multiplication. Nat New Biol. 1973 Dec 12;246(154):181–183. doi: 10.1038/newbio246181a0. [DOI] [PubMed] [Google Scholar]
  4. Freudenberg H., Mager J. Studies on the mechanism of the inhibition of protein synthesis induced by intracellular ATP depletion. Biochim Biophys Acta. 1971 Mar 25;232(3):537–555. doi: 10.1016/0005-2787(71)90608-3. [DOI] [PubMed] [Google Scholar]
  5. Halperin M. L., Connors H. P., Relman A. S., Karnovsky M. L. Factors that control the effect of pH on glycolysis in leukocytes. J Biol Chem. 1969 Jan 25;244(2):384–390. [PubMed] [Google Scholar]
  6. Hatanaka M., Huebner R. J., Gilden R. V. Alterations in the characteristics of sugar uptake by mouse cells transformed by murine sarcoma viruses. J Natl Cancer Inst. 1969 Nov;43(5):1091–1096. [PubMed] [Google Scholar]
  7. Hatanaka M. Transport of sugars in tumor cell membranes. Biochim Biophys Acta. 1974 Apr 29;355(1):77–104. doi: 10.1016/0304-419x(74)90008-0. [DOI] [PubMed] [Google Scholar]
  8. IBSEN K. H., COE E. L., McKEE R. W. Some factors influencing respiration and glycolysis in Ehrlich ascites tumor cells. Cancer Res. 1960 Oct;20:1399–1407. [PubMed] [Google Scholar]
  9. Kaminskas E., Field M., Henshaw E. C. Cyclic AMP and growth of Ehrlich ascites tumor cells. Lack of cyclic AMP elevation in nutritionally deprived cells and mechanism of retardation of growth by dibutryl cyclic AMP. Biochim Biophys Acta. 1976 Sep 24;444(2):539–553. doi: 10.1016/0304-4165(76)90398-6. [DOI] [PubMed] [Google Scholar]
  10. Kaminskas E. Inactivation of protein synthesis stimulating activity in serum by cells. J Cell Physiol. 1973 Dec;82(3):475–488. doi: 10.1002/jcp.1040820316. [DOI] [PubMed] [Google Scholar]
  11. Kaminskas E. Serum-mediated stimulation of protein synthesis in Ehrlich ascites tumor cells. J Biol Chem. 1972 Sep 10;247(17):5470–5476. [PubMed] [Google Scholar]
  12. Kletzien R. F., Perdue J. F. Sugar transport in chick embryo fibroblasts. I. A functional change in the plasma membrane associated with the rate of cell growth. J Biol Chem. 1974 Jun 10;249(11):3366–3374. [PubMed] [Google Scholar]
  13. LETNANSKY K. THE INFLUENCE OF 2-DEOXY-D-GLUCOSE ON THE NUCLEOTIDE CONTENT OF EHRLICH ASCITES CARCINOMA CELLS. Biochim Biophys Acta. 1964 May 18;87:1–8. doi: 10.1016/0926-6550(64)90040-4. [DOI] [PubMed] [Google Scholar]
  14. Live T. R., Kaminskas E. Changes in adenylate energy charge in Ehrlich ascites tumor cells deprived of serum, glucose, or amino acids. J Biol Chem. 1975 Mar 10;250(5):1786–1789. [PubMed] [Google Scholar]
  15. MCCOMB R. B., YUSHOK W. D. METABOLISM OF ASCITES TUMOR CELLS. III. EFFECT OF 2-DEOXYGLUCOSE PHOSPHORYLATION ON PHOSPHORUS METABOLISM. Cancer Res. 1964 Feb;24:193–197. [PubMed] [Google Scholar]
  16. Plagemann P. G., Richey D. P. Transport of nucleosides, nucleic acid bases, choline and glucose by animal cells in culture. Biochim Biophys Acta. 1974 Dec 16;344(3-4):263–305. doi: 10.1016/0304-4157(74)90010-0. [DOI] [PubMed] [Google Scholar]
  17. Renner E. D., Plagemann P. G., Bernlohr R. W. Permeation of glucose by simple and facilitated diffusion by Novikoff rat hepatoma cells in suspension culture and its relationship to glucose metabolism. J Biol Chem. 1972 Sep 25;247(18):5765–5776. [PubMed] [Google Scholar]
  18. Rubin H. pH and population density in the regulation of animal cell multiplication. J Cell Biol. 1971 Dec;51(3):686–702. doi: 10.1083/jcb.51.3.686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schmidt M. F., Schwarz R. T., Scholtissek C. Nucleoside-diphosphate derivatives of 2-deoxy-D-glucose in animal cells. Eur J Biochem. 1974 Nov 1;49(1):237–247. doi: 10.1111/j.1432-1033.1974.tb03828.x. [DOI] [PubMed] [Google Scholar]
  20. Ui M. A role of phosphofructokinase in pH-dependent regulation of glycolysis. Biochim Biophys Acta. 1966 Aug 24;124(2):310–322. doi: 10.1016/0304-4165(66)90194-2. [DOI] [PubMed] [Google Scholar]
  21. Van Ventrooij W. J., Henshaw E. C., Hirsch C. A. Nutritional effects on the polyribosome distribution and rate of protein synthesis in Ehrlich ascites tumor cells in culture. J Biol Chem. 1970 Nov 25;245(22):5947–5953. [PubMed] [Google Scholar]
  22. Venuta S., Rubin H. Sugar transport in normal and Rous sarcoma virus-transformed chick-embryo fibroblasts. Proc Natl Acad Sci U S A. 1973 Mar;70(3):653–657. doi: 10.1073/pnas.70.3.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wilhelm Gerhard, Schulz Jürgen, Hofmann Eberhard. pH-dependence of aerobic glycolysis in ehrlich ascites tumour cells. FEBS Lett. 1971 Sep 15;17(1):158–162. doi: 10.1016/0014-5793(71)80587-2. [DOI] [PubMed] [Google Scholar]
  24. ZWARTOUW H. T., WESTWOOD J. C. Factors affecting growth and glycolysis in tissue culture. Br J Exp Pathol. 1958 Oct;39(5):529–539. [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES