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. 1978 Sep 15;174(3):1079–1082. doi: 10.1042/bj1741079

Interference by branched-chain amino acids in the assay of alanine with alanine dehydrogenase.

P Lund, G Baverel
PMCID: PMC1186017  PMID: 728077

Abstract

Commercial preparations of alanine dehydrogenase from Bacillus subtilis are contaminated to varying extents with activity towards branched-chain amino acids. The Km values for these amino acids are of the same order as for L-alanine (about 10(-3)M). The branched-chain amino acid dehydrogenase activity is lost on dialysis for 2--4h against water or 2mM-EDTA.

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Selected References

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  1. Felig P. Symposium: Controlling diabetes mellitus with diet therapy. Introduction. Managing diabetes. Postgrad Med. 1976 Jan;59(1):113–113. doi: 10.1080/00325481.1976.11716528. [DOI] [PubMed] [Google Scholar]
  2. Goldstein L., Newsholme E. A. The formation of alanine from amino acids in diaphragm muscle of the rat. Biochem J. 1976 Feb 15;154(2):555–558. doi: 10.1042/bj1540555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Krebs H. A., Lund P. Aspects of the regulation of the metabolism of branched-chain amino acids. Adv Enzyme Regul. 1976;15:375–394. doi: 10.1016/0065-2571(77)90026-7. [DOI] [PubMed] [Google Scholar]
  4. MEISTER A. Enzymatic preparation of alpha-keto acids. J Biol Chem. 1952 May;197(1):309–317. [PubMed] [Google Scholar]
  5. Odessey R., Khairallah E. A., Goldberg A. L. Origin and possible significance of alanine production by skeletal muscle. J Biol Chem. 1974 Dec 10;249(23):7623–7629. [PubMed] [Google Scholar]
  6. Pozefsky T., Felig P., Tobin J. D., Soeldner J. S., Cahill G. F., Jr Amino acid balance across tissues of the forearm in postabsorptive man. Effects of insulin at two dose levels. J Clin Invest. 1969 Dec;48(12):2273–2282. doi: 10.1172/JCI106193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ruderman N. B., Berger M. The formation of glutamine and alanine in skeletal muscle. J Biol Chem. 1974 Sep 10;249(17):5500–5506. [PubMed] [Google Scholar]
  8. Ruderman N. B., Lund P. Amino acid metabolism in skeletal muscle. Regulation of glutamine and alanine release in the perfused rat hindquarter. Isr J Med Sci. 1972 Mar;8(3):295–302. [PubMed] [Google Scholar]
  9. Ruderman N. B. Muscle amino acid metabolism and gluconeogenesis. Annu Rev Med. 1975;26:245–258. doi: 10.1146/annurev.me.26.020175.001333. [DOI] [PubMed] [Google Scholar]
  10. Snell K., Duff D. A. The release of alanine by rat diaphragm muscle in vitro. Biochem J. 1977 Feb 15;162(2):399–403. doi: 10.1042/bj1620399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Williamson D. H., Lopes-Vieira O., Walker B. Concentrations of free glucogenic amino acids in livers of rats subjected to various metabolic stresses. Biochem J. 1967 Aug;104(2):497–502. doi: 10.1042/bj1040497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. YOSHIDA A., FREESE E. ENZYMATIC PROPERTIES OF ALANINE DEHYDROGENASE OF BACILLUS SUBTILIS. Biochim Biophys Acta. 1965 Feb 22;96:248–262. [PubMed] [Google Scholar]

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