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. 1978 Sep 15;174(3):1083–1086. doi: 10.1042/bj1741083

Sodium-dependent alanine transport in plasma-membrane vesicles from rat liver.

J M van Amelsvoort, H J Sips, K van Dam
PMCID: PMC1186018  PMID: 728078

Abstract

L-Alanine transport was studied in plasma-membrane vesicles from rat liver. A gradient of NaSCN, but not of KSCN, stimulated alanine uptake. Monensin plus carbonyl cyanide p-trifluoromethoxyphenylhydrazone abolished the observed overshoot in uptake. After equilibration of alanine, NaSCN induced uphill transport.

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Selected References

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  1. Bachmann W., Challoner D. D-glucose uptake by a rat liver plasma membrane preparation. Biochim Biophys Acta. 1976 Aug 16;443(2):254–266. doi: 10.1016/0005-2736(76)90508-3. [DOI] [PubMed] [Google Scholar]
  2. Chambers J. W., Georg R. H., Bass A. D. Effect of glucagon, cyclic 3',5'-adenosine monophosphate and its dibutyryl derivative on amino acid uptake by the isolated perfused rat liver. Endocrinology. 1970 Aug;87(2):366–370. doi: 10.1210/endo-87-2-366. [DOI] [PubMed] [Google Scholar]
  3. Donner D. B., Nakayama K., Lutz U., Sonenberg M. The effects of bioregulators upon amino acid transport and protein synthesis in isolated rat hepatocytes. Biochim Biophys Acta. 1978 Feb 21;507(2):322–336. doi: 10.1016/0005-2736(78)90426-1. [DOI] [PubMed] [Google Scholar]
  4. Edmondson J. W., Lumeng L., Li T. K. Direct measurement of active transport systems for alanine in freshly isolated rat liver cells. Biochem Biophys Res Commun. 1977 Jun 6;76(3):751–757. doi: 10.1016/0006-291x(77)91564-9. [DOI] [PubMed] [Google Scholar]
  5. Evers J., Murer H., Kinne R. Phenylalanine uptake in isolated renal brush border vesicles. Biochim Biophys Acta. 1976 Apr 5;426(4):598–615. doi: 10.1016/0005-2736(76)90124-3. [DOI] [PubMed] [Google Scholar]
  6. FLECK A., MUNRO H. N. The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimation. Biochim Biophys Acta. 1962 May 14;55:571–583. doi: 10.1016/0006-3002(62)90836-3. [DOI] [PubMed] [Google Scholar]
  7. Le Cam A., Freychet P. Neutral amino acid transport. Characterization of the A and L systems in isolated rat hepatocytes. J Biol Chem. 1977 Jan 10;252(1):148–156. [PubMed] [Google Scholar]
  8. Lücke H., Haase W., Murer H. Amino acid transport in brush-border-membrane vesicles isolated from human small intestine. Biochem J. 1977 Dec 15;168(3):529–532. doi: 10.1042/bj1680529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MYERS D. K., SLATER E. C. The enzymic hydrolysis of adenosine triphosphate by liver mitochondria. I. Activities at different pH values. Biochem J. 1957 Dec;67(4):558–572. doi: 10.1042/bj0670558. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McGivan J. D., Bradford N. M., Mendes-Mourão J. The transport of branched-chain amino acids into isolated rat liver cells. FEBS Lett. 1977 Aug 15;80(2):380–384. doi: 10.1016/0014-5793(77)80481-x. [DOI] [PubMed] [Google Scholar]
  11. Neville D. M., Jr Isolation of an organ specific protein antigen from cell-surface membrane of rat liver. Biochim Biophys Acta. 1968 Apr 9;154(3):540–552. doi: 10.1016/0005-2795(68)90014-7. [DOI] [PubMed] [Google Scholar]
  12. Ray T. K. A modified method for the isolation of the plasma membrane from rat liver. Biochim Biophys Acta. 1970 Jan 6;196(1):1–9. doi: 10.1016/0005-2736(70)90159-8. [DOI] [PubMed] [Google Scholar]
  13. Seglen P. O., Solheim A. E. Valine uptake and incorporation into protein in isolated rat hepatocytes. Nature of the precursor pool for protein synthesis. Eur J Biochem. 1978 Apr;85(1):15–25. doi: 10.1111/j.1432-1033.1978.tb12208.x. [DOI] [PubMed] [Google Scholar]
  14. Slack E. N., Liang T., Sacktor B. Transport of L-proline and D-glucose in luminal (brush border) and contraluminal (basal-lateral) membrane vesicles from the renal cortex. Biochem Biophys Res Commun. 1977 Aug 8;77(3):891–897. doi: 10.1016/s0006-291x(77)80061-2. [DOI] [PubMed] [Google Scholar]
  15. Tews J. K., Colosi N. W., Harper A. E. Effect of amino acid meals on hepatic alpha-aminoisobutyrate transport and cyclic AMP. Am J Physiol. 1975 May;228(5):1606–1614. doi: 10.1152/ajplegacy.1975.228.5.1606. [DOI] [PubMed] [Google Scholar]
  16. Tyrakowski T., Milutinović S., Schulz I. Studies on isolated subcellular components of cat pancreas. III. Alanine-sodium cotransport in isolated plasma membrane vesicles. J Membr Biol. 1978 Feb 3;38(4):333–346. doi: 10.1007/BF01870150. [DOI] [PubMed] [Google Scholar]
  17. Wisher M. H., Evans W. H. Functional polarity of the rat hepatocyte surface membrane. Isolation and characterization of plasma-membrane subfractions from the blood-sinusoidal, bile-Canalicular and contiguous surfaces of the hepatocyte. Biochem J. 1975 Feb;146(2):375–388. doi: 10.1042/bj1460375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. van Amelsvoort J. M., de Pont J. J., Bonting S. L. Is there a plasma membrane-located anion-sensitive ATPase? Biochim Biophys Acta. 1977 Apr 18;466(2):283–301. doi: 10.1016/0005-2736(77)90225-5. [DOI] [PubMed] [Google Scholar]

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