Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1978 Oct 1;175(1):345–347. doi: 10.1042/bj1750345

Incorporation of [18O]water in the formation of p-hydroxybenzyl alcohol by the p-cresol methylhydroxylase from Pseudomonas putida.

D J Hopper
PMCID: PMC1186072  PMID: 736904

Abstract

In the hydroxylation of the methyl group of p-cresol by an enzyme from Pseudomonas putida the oxygen atom is derived from water. Although a second reaction by the same enzyme converts the product, p-hydroxybenzyl alcohol, into the aldehyde, the alcohol is an enzyme-free intermediate.

Full text

PDF
345

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bernhardt F. H., Pachowsky H., Staudinger H. A 4-methoxybenzoate O-demethylase from Pseudomonas putida. A new type of monooxygenase system. Eur J Biochem. 1975 Sep 1;57(1):241–256. doi: 10.1111/j.1432-1033.1975.tb02296.x. [DOI] [PubMed] [Google Scholar]
  2. Chapman P. J., Hopper D. J. The bacterial metabolism of 2,4-xylenol. Biochem J. 1968 Dec;110(3):491–498. doi: 10.1042/bj1100491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hopper D. J., Taylor D. G. The purification and properties of p-cresol-(acceptor) oxidoreductase (hydroxylating), a flavocytochrome from Pseudomonas putida. Biochem J. 1977 Oct 1;167(1):155–162. doi: 10.1042/bj1670155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hopper D. J. The hydroxylation of P-cresol and its conversion to P-hydroxybenzaldehyde in Pseudomonas putida. Biochem Biophys Res Commun. 1976 Mar 22;69(2):462–468. doi: 10.1016/0006-291x(76)90544-1. [DOI] [PubMed] [Google Scholar]
  5. McKenna E. J., Coon M. J. Enzymatic omega-oxidation. IV. Purification and properties of the omega-hydroxylase of Pseudomonas oleovorans. J Biol Chem. 1970 Aug 10;245(15):3882–3889. [PubMed] [Google Scholar]
  6. Toms A., Wood J. M. Early intermediates in the degradation of alpha-conidendrin by a Pseudomonas multivorans. Biochemistry. 1970 Feb 17;9(4):733–740. doi: 10.1021/bi00806a005. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES