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. 1978 Nov 1;175(2):607–612. doi: 10.1042/bj1750607

Modification by liposomes of the adenosine triphosphate-activating effect on adenylate deaminase from pig heart.

J Purzycka-Preis, E Prus, M Woźniak, M Zydowo
PMCID: PMC1186110  PMID: 743213

Abstract

Adenylate deaminase (AMP deaminase, EC 3.5.4.6) of a high substrate specificity was purified from pig heart by chromatography on cellulose phosphate. The enzyme shows a co-operative binding of AMP [h (Hill coefficient) 2.35, with SO.5 (half-saturating substrate concentration) 5mM]. ATP and ADP act as positive effectors, lowering h to 1.55 and SO.5 to 1 mM. The addition of liposomes (phospholipid bilayers) to ATP-activated or ADP-activated enzyme causes a further shift of the h value to 1.04 and SO.5 to 0.5 mM. For ATP-activated enzyme the addition of liposomes increases Vmax. by about 100%, and for ADP-activated enzyme by 50%. Liposomes have no effect on the kinetics of AMP deaminase in the absence of ATP and ADP, and neither do they influence the inhibitory effect of orthophosphate on heart muscle AMP deaminase. Metabolic implications of these findings are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashby B., Frieden C. Interaction of AMP-aminohydrolase with myosin and its subfragments. J Biol Chem. 1977 Mar 25;252(6):1869–1872. [PubMed] [Google Scholar]
  2. BERNE R. M. REGULATION OF CORONARY BLOOD FLOW. Physiol Rev. 1964 Jan;44:1–29. doi: 10.1152/physrev.1964.44.1.1. [DOI] [PubMed] [Google Scholar]
  3. Boosman A., Chilson O. P. Subunit structure of AMP-deaminase from chicken and rabbit skeletal muscle. J Biol Chem. 1976 Apr 10;251(7):1847–1852. [PubMed] [Google Scholar]
  4. Burger R., Lowenstein J. M. Adenylate deaminase. 3. Regulation of deamination pathways in extracts of rat heart and lung. J Biol Chem. 1967 Nov 25;242(22):5281–5288. [PubMed] [Google Scholar]
  5. Byrnes E. W., Suelter C. H. Preparation of rabbit muscle myosin or actomyosin free of 5'-adenylic acid deaminase. Biochem Biophys Res Commun. 1965 Aug 16;20(4):422–426. doi: 10.1016/0006-291x(65)90594-2. [DOI] [PubMed] [Google Scholar]
  6. CHANEY A. L., MARBACH E. P. Modified reagents for determination of urea and ammonia. Clin Chem. 1962 Apr;8:130–132. [PubMed] [Google Scholar]
  7. CURRIE R. D., WEBSTER H. L. Preparation of 5'-adenylic acid deaminase based on phosphate-induced dissociation of rat actomyosin-deaminase complexes. Biochim Biophys Acta. 1962 Oct 8;64:30–40. doi: 10.1016/0006-3002(62)90757-6. [DOI] [PubMed] [Google Scholar]
  8. Chung L., Bridger W. A. Activation of rabbit cardiac AMP aminohydrolase by ADP: a component of a mechanism guarding against ATP depletion. FEBS Lett. 1976 May 1;64(2):338–340. doi: 10.1016/0014-5793(76)80323-7. [DOI] [PubMed] [Google Scholar]
  9. Coffee C. J., Kofke W. A. Rat muscle 5'-adenylic acid aminohydrolase. I. Purification and subunit structure. J Biol Chem. 1975 Sep 10;250(17):6653–6658. [PubMed] [Google Scholar]
  10. Entman M. L., Bornet E. P., Van Winkle W. B., Goldstein M. A., Schwartz A. Association of glycogenolysis with cardiac sarcoplasmic reticulum: II. Effect of glycogen depletion, deoxycholate solubilization and cardiac ischemia: evidence for a phorphorylase kinase membrane complex. J Mol Cell Cardiol. 1977 Jul;9(7):515–528. doi: 10.1016/s0022-2828(77)80367-2. [DOI] [PubMed] [Google Scholar]
  11. Galla H. J., Sackmann E. Chemically induced lipid phase separation in model membranes containing charged lipids: a spin label study. Biochim Biophys Acta. 1975 Sep 2;401(3):509–529. doi: 10.1016/0005-2736(75)90249-7. [DOI] [PubMed] [Google Scholar]
  12. Gibbs K. L., Bishop S. H. Adenosine triphosphate-activated adenylate deaminase from marine invertebrate animals. Properties of the enzyme from lugworm (Arenicola cristata) body-wall muscle. Biochem J. 1977 Jun 1;163(3):511–516. doi: 10.1042/bj1630511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Ogasawara N., Goto H., Watanabe T. Isozymes of rat AMP deaminase. Biochim Biophys Acta. 1975 Oct 22;403(2):530–537. doi: 10.1016/0005-2744(75)90081-9. [DOI] [PubMed] [Google Scholar]
  15. Ogasawara N., Goto H., Watanabe T., Kawamura Y., Yoshino M. AMP deaminase from rat brain: purification and characterization of multiple forms. Biochim Biophys Acta. 1974 Oct 17;364(2):353–364. doi: 10.1016/0005-2744(74)90020-5. [DOI] [PubMed] [Google Scholar]
  16. PURZYCKA J. AMP and adenosine aminohydrolases in rat tissues. Acta Biochim Pol. 1962;9:83–93. [PubMed] [Google Scholar]
  17. Setlow B., Lowenstein J. M. Adenylate deaminase. II. Purification and some regulatory properties of the enzyme from calf brain. J Biol Chem. 1967 Feb 25;242(4):607–615. [PubMed] [Google Scholar]
  18. Smiley K. L., Jr, Berry A. J., Suelter C. H. An improved purification, crystallization, and some properties of rabbit muscle 5'-adenylic acid deaminase. J Biol Chem. 1967 May 25;242(10):2502–2506. [PubMed] [Google Scholar]
  19. Smiley K. L., Suelter C. H. Univalent cations as allosteric activators of muscle adenosine 5'-phosphate deaminase. J Biol Chem. 1967 Apr 25;242(8):1980–1981. [PubMed] [Google Scholar]
  20. Wooster M. S., Wrigglesworth J. M. Modification of glyceraldehyde 3-phosphate dehydrogenase activity by adsorption on phospholipid vesicles. Biochem J. 1976 Dec 1;159(3):627–631. doi: 10.1042/bj1590627. [DOI] [PMC free article] [PubMed] [Google Scholar]

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