Abstract
It is demonstrated that exclusion phenomena appear to dominate the interaction of dextran with albumin in aqueous solution. The enthalpic contribution to the interaction coefficient describing dextran/albumin mixtures is small, although its determination was subject to considerable error. These results support the earlier assumptions of the type of interaction between the two polymers. The conclusions are primarily based on the interpretation of the temperature-dependence of the interaction coefficient, as measured by light-scattering in the temperature range 6--33 degrees C. Enthalpy of dilution measurements of dextran/albumin mixtures by microcalorimetry were in qualitative agreement with the light-scattering data.
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Selected References
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- Comper W. D., Laurent T. C. Physiological function of connective tissue polysaccharides. Physiol Rev. 1978 Jan;58(1):255–315. doi: 10.1152/physrev.1978.58.1.255. [DOI] [PubMed] [Google Scholar]
- Edmond E., Ogston A. G. An approach to the study of phase separation in ternary aqueous systems. Biochem J. 1968 Oct;109(4):569–576. doi: 10.1042/bj1090569. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LAURENT T. C., OGSTON A. G. THE INTERACTION BETWEEN POLYSACCHARIDES AND OTHER MACROMOLECULES. 4. THE OSMOTIC PRESSURE OF MIXTURES OF SERUM ALBUMIN AND HYALURONIC ACID. Biochem J. 1963 Nov;89:249–253. doi: 10.1042/bj0890249. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laurent T. C., Obrink B. On the restriction of the rotational diffusion of proteins in polymer networks. Eur J Biochem. 1972 Jun 23;28(1):94–101. doi: 10.1111/j.1432-1033.1972.tb01888.x. [DOI] [PubMed] [Google Scholar]
- Laurent T. C., Preston B. N., Carlsson B. Conformational transitions of polynucleotides in polymer media. Eur J Biochem. 1974 Apr 1;43(2):231–235. doi: 10.1111/j.1432-1033.1974.tb03404.x. [DOI] [PubMed] [Google Scholar]
- Laurent T. C. The interaction between polysaccharides and other macromolecules. 9. The exclusion of molecules from hyaluronic acid gels and solutions. Biochem J. 1964 Oct;93(1):106–112. doi: 10.1042/bj0930106. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meyer F. A., Comper W. D., Preston B. N. Model connective tissue systems. A physical study of gelatin gels containing proteoglycans. Biopolymers. 1971;10(8):1351–1364. doi: 10.1002/bip.360100808. [DOI] [PubMed] [Google Scholar]
- OGSTON A. G., PHELPS C. F. The partition of solutes between buffer solutions and solutions containing hyaluronic acid. Biochem J. 1961 Apr;78:827–833. doi: 10.1042/bj0780827. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ogston A. G., Preston B. N. The exclusion of protein by hyaluronic acid. Measurement by light scattering. J Biol Chem. 1966 Jan 10;241(1):17–19. [PubMed] [Google Scholar]
- Ogston A. G., Silpananta P. The thermodynamics of interaction between Sephadex and penetrating solutes. Biochem J. 1970 Jan;116(2):171–175. doi: 10.1042/bj1160171. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Preston B. N., Davies M., Ogston A. G. The composition and physicochemical properties of hyaluronic acids prepared from ox synovial fluid and from a case of mesothelioma. Biochem J. 1965 Aug;96(2):449–474. doi: 10.1042/bj0960449. [DOI] [PMC free article] [PubMed] [Google Scholar]