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. 1978 Oct 15;176(1):169–174. doi: 10.1042/bj1760169

Stimulation of triacylglycerol synthesis in rat adipocytes by plasma very-low-density lipoproteins.

P Thomopoulos, M Berthelier, D Lagrange, M J Chapman, M H Laudat
PMCID: PMC1186217  PMID: 215128

Abstract

The effect of human plasma lipoproteins on lipogenesis from glucose has been studied in isolated rat adipocytes. The very-low-density lipoproteins increased lipogenesis specifically, whereas low-density lipoproteins and high-density lipoproteins were without effect. Such stimulation could be reproduced with partially delipidated very-low-density lipoproteins. Nod-esterified fatty acids and glycerol were also without effect. Pretreatment of the adipocytes with trypsin did not alter the effect of very-low-density lipoprotein. The presence of Ca2+ was required for the full activation of lipogenesis. The synthesis of acylglycerol fatty acids and of acylglycerol glycerol were equally increased. The effect of very-low-density lipoprotein was not additive to that of insulin. It is suggested that very-low-density lipoprotein may directly stimulate lipogenesis in fat-cells, particularly in states when the lipoproteins are present at high concentration in the circulation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachorik P. S., Livingston J. N., Cooke J., Kwiterovich P. O. The binding of low density lipoprotein by liver membranes in the pig. Biochem Biophys Res Commun. 1976 Apr 19;69(4):927–935. doi: 10.1016/0006-291x(76)90462-9. [DOI] [PubMed] [Google Scholar]
  2. Bersot T. P., Brown W. V., Levy R. I., Windmueller H. G., Fredrickson D. S., LeQuire V. S. Further characterization of the apolipoproteins of rat plasma lipoproteins. Biochemistry. 1970 Aug 18;9(17):3427–3433. doi: 10.1021/bi00819a022. [DOI] [PubMed] [Google Scholar]
  3. Biggs H. G., Erikson J. M., Moorehead W. R. A manual colormetric assay of triglycerides in serum. Clin Chem. 1975 Mar;21(3):437–441. [PubMed] [Google Scholar]
  4. Brown M. S., Goldstein J. L. Receptor-mediated control of cholesterol metabolism. Science. 1976 Jan 16;191(4223):150–154. doi: 10.1126/science.174194. [DOI] [PubMed] [Google Scholar]
  5. Chapman M. J., Goldstein S. Comparison of the serum low density lipoprotein and of its apoprotein in the pig, rhesus monkey and baboon with that in man. Atherosclerosis. 1976 Nov-Dec;25(2-3):267–291. doi: 10.1016/0021-9150(76)90033-2. [DOI] [PubMed] [Google Scholar]
  6. Czech M. P., Lawrence J. C., Jr, Lynn W. S. Evidence for electron transfer reactions involved in the Cu2+ -dependent thiol activation of fat cell glucose utilization. J Biol Chem. 1974 Feb 25;249(4):1001–1006. [PubMed] [Google Scholar]
  7. Fain J. N., Shepherd R. E. Free fatty acids as feedback regulators of adenylate cyclase and cyclic 3':5'-AMP accumulation in rat fat cells. J Biol Chem. 1975 Aug 25;250(16):6586–6592. [PubMed] [Google Scholar]
  8. Galton D. J. Lipogenesis in human adipose tissue. J Lipid Res. 1968 Jan;9(1):19–26. [PubMed] [Google Scholar]
  9. Gliemann J., Osterlind K., Vinten J., Gammeltoft S. A procedure for measurement of distribution spaces in isolated fat cells. Biochim Biophys Acta. 1972 Nov 24;286(1):1–9. doi: 10.1016/0304-4165(72)90082-7. [DOI] [PubMed] [Google Scholar]
  10. Goldrick R. B., Ashley B. C., Lloyd M. L. Effects of prolonged incubation and cell concentration on lipogenesis from glucose in isolated human omental fat cells. J Lipid Res. 1969 May;10(3):253–259. [PubMed] [Google Scholar]
  11. HAVEL R. J., EDER H. A., BRAGDON J. H. The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J Clin Invest. 1955 Sep;34(9):1345–1353. doi: 10.1172/JCI103182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jacobs R. A., Majerus P. W. The regulation of fatty acid synthesis in human skin fibroblasts. Inhibition of fatty acid synthesis by free fatty acids. J Biol Chem. 1973 Dec 25;248(24):8392–8401. [PubMed] [Google Scholar]
  13. Jacobs R. A., Sly W. S., Majerus P. W. The regulation of fatty acid biosynthesis in human skin fibroblasts. J Biol Chem. 1973 Feb 25;248(4):1268–1276. [PubMed] [Google Scholar]
  14. Kissebah A. H., Tulloch B. R., Hope-Gill H., Clarke P. V., Vydelingum N., Fraser T. R. Mode of insulin action. Lancet. 1975 Jan 18;1(7899):144–147. doi: 10.1016/s0140-6736(75)91435-x. [DOI] [PubMed] [Google Scholar]
  15. Kono T., Barham F. W. Insulin-like effects of trypsin on fat cells. Localization of the metabolic steps and the cellular site affected by the enzyme. J Biol Chem. 1971 Oct 25;246(20):6204–6209. [PubMed] [Google Scholar]
  16. Kono T. Destruction and restoration of the insulin effector system of isolated fat cells. J Biol Chem. 1969 Nov 10;244(21):5777–5784. [PubMed] [Google Scholar]
  17. Krishnaiah K. V., Wiegandt H. Demonstration of protease-like activity in human serum low density lipoprotein. FEBS Lett. 1974 Apr 1;40(2):265–268. doi: 10.1016/0014-5793(74)80241-3. [DOI] [PubMed] [Google Scholar]
  18. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  19. Lakshmanan M. R., Muesing R. A., Cook G. A., Veech R. L. Regulation of lipogenesis in isolated hepatocytes by triglyceride-rich lipoproteins. J Biol Chem. 1977 Oct 10;252(19):6581–6584. [PubMed] [Google Scholar]
  20. Livingston J. N., Gurny P. A., Lockwood D. H. Insulin-like effects of polyamines in fat cells. Mediation by H2O2 formation. J Biol Chem. 1977 Jan 25;252(2):560–562. [PubMed] [Google Scholar]
  21. Mayes P. A., Topping D. L. Regulation of hepatic lipogenesis by plasma free fatty acids: simultaneous studies on lipoprotein secretion, cholesterol synthesis, ketogenesis and gluconeogenesis. Biochem J. 1974 Apr;140(1):111–114. doi: 10.1042/bj1400111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Mills G. L., Taylaur C. E. The distribution and composition of serum lipoproteins in eighteen animals. Comp Biochem Physiol B. 1971 Oct;40(2):489–501. doi: 10.1016/0305-0491(71)90234-3. [DOI] [PubMed] [Google Scholar]
  23. Moody A. J., Stan M. A., Stan M., Gliemann J. A simple free fat cell bioassay for insulin. Horm Metab Res. 1974 Jan;6(1):12–16. doi: 10.1055/s-0028-1093895. [DOI] [PubMed] [Google Scholar]
  24. Morrisett J. D., Jackson R. L., Gotto A. M., Jr Lipoproteins: structure and function. Annu Rev Biochem. 1975;44:183–207. doi: 10.1146/annurev.bi.44.070175.001151. [DOI] [PubMed] [Google Scholar]
  25. Nestel P. J. Triglyceride turnover in man. Effects of dietary carbohydrate. Prog Biochem Pharmacol. 1973;8:125–160. [PubMed] [Google Scholar]
  26. Pairault J., Levilliers J., Chapman M. J. Human serum lipoproteins activate adipocyte plasma membrane adenylate cyclase. Nature. 1977 Oct 13;269(5629):607–609. doi: 10.1038/269607a0. [DOI] [PubMed] [Google Scholar]
  27. RODBELL M. METABOLISM OF ISOLATED FAT CELLS. I. EFFECTS OF HORMONES ON GLUCOSE METABOLISM AND LIPOLYSIS. J Biol Chem. 1964 Feb;239:375–380. [PubMed] [Google Scholar]
  28. Reichl D., Simons L. A., Myant N. B., Pflug J. J., Mills G. L. The lipids and lipoproteins of human peripheral lymph, with observations on the transport of cholesterol from plasma and tissues into lymph. Clin Sci Mol Med. 1973 Sep;45(3):313–329. doi: 10.1042/cs0450313. [DOI] [PubMed] [Google Scholar]
  29. Rerabek J. E. Low density lipoproteins and immunoglobulins in human pleural effusions. Clin Chim Acta. 1977 May 2;76(3):363–369. doi: 10.1016/0009-8981(77)90163-2. [DOI] [PubMed] [Google Scholar]
  30. Saggerson E. D. The regulation of glyceride synthesis in isolated white-fat cells. The effects of palmitate and lipolytic agents. Biochem J. 1972 Aug;128(5):1057–1067. doi: 10.1042/bj1281057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Shore B., Shore V. Isolation and characterization of polypeptides of human serum lipoproteins. Biochemistry. 1969 Nov;8(11):4510–4516. doi: 10.1021/bi00839a043. [DOI] [PubMed] [Google Scholar]
  32. Shore V., Shore B. Stimulation of human erythrocyte Mg++ -ATPASE by plasma lipoproteins1. Biochem Biophys Res Commun. 1975 Aug 18;65(4):1250–1256. doi: 10.1016/s0006-291x(75)80364-0. [DOI] [PubMed] [Google Scholar]
  33. Smith U. Effect of cell size on lipid synthesis by human adipose tissue in vitro. J Lipid Res. 1971 Jan;12(1):65–70. [PubMed] [Google Scholar]
  34. Sutherland W. H., Larking P. W., Nye E. R. Modification of nicotinic acid and prostaglandin E1 antilipolytic action in vitro. Atherosclerosis. 1976 Oct;25(1):45–53. doi: 10.1016/0021-9150(76)90046-0. [DOI] [PubMed] [Google Scholar]
  35. Swaney J. B., Braithwaite F., Eder H. A. Characterization of the apolipoproteins of rat plasma lipoproteins. Biochemistry. 1977 Jan 25;16(2):271–278. doi: 10.1021/bi00621a018. [DOI] [PubMed] [Google Scholar]
  36. el-Allawy R. M., Gliemann J. Trypsin treatment of adipocytes: effect on sensitivity to insulin. Biochim Biophys Acta. 1972 Jun 26;273(1):97–109. doi: 10.1016/0304-4165(72)90196-1. [DOI] [PubMed] [Google Scholar]

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