Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1978 Nov 15;176(2):599–602. doi: 10.1042/bj1760599

Recognition of the beta-subunit of human chorionic gonadotropin and sub-determinants by target tissue receptors.

S Ramakrishnan, C Das, G P Talwar
PMCID: PMC1186271  PMID: 217366

Abstract

The beta-subunit of human chorionic gonadotropin, purified immunochemically to eliminate undissociated human chorionic gonadotropin, induced testosterone production by mouse Leydig cells at concentrations 400-fold higher than human chorionic gonadotropin. Steroidogenesis was also stimulated by a synthetic fragment of the beta-subunit of human chorionic gonadotropin conforming to the peptide sequence residues 39--71, whereas peptide sequence residues 39--56 and three C-terminal fragments (residues 115--145, 111--145 and 101--145) failed to cause steroidogenesis. These studies suggest the presence in the beta-subunit of human chorionic gonadotropin of determinants recognized by the tissue receptors, a part of these determinants residing between amino acid residues 57--71.

Full text

PDF
599

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Canfield R. E., Morgan F. J., Kammerman S., Bell J. J., Agosto G. M. Studies of human chorionic gonadotropin. Recent Prog Horm Res. 1971;27:121–164. doi: 10.1016/b978-0-12-571127-2.50028-6. [DOI] [PubMed] [Google Scholar]
  2. Catt K. J., Dufau M. L. Spare gonadotrophin receptors in rat testis. Nat New Biol. 1973 Aug 15;244(137):219–221. doi: 10.1038/newbio244219a0. [DOI] [PubMed] [Google Scholar]
  3. Channing C. P., Kammerman S. Effects of hCG, asialo-hCG and the subunits of hCG upon luteinization of monkey granulosa cell cultures. Endocrinology. 1973 Nov;93(5):1035–1043. doi: 10.1210/endo-93-5-1035. [DOI] [PubMed] [Google Scholar]
  4. Dufau M. L., Mendelson C. R., Catt K. J. A highly sensitive in vitro bioassay for luteinizing hormone and chorionic gonadotropin: testosterone production by dispersed Leydig cells. J Clin Endocrinol Metab. 1974 Sep;39(3):610–613. doi: 10.1210/jcem-39-3-610. [DOI] [PubMed] [Google Scholar]
  5. Farmer S. W., Sairam M. R., Papkoff H. Stimulation of lactic acid production in vitro from prepubertal rat ovaries by gonadotropins and their subunits. Endocrinology. 1973 Apr;92(4):1022–1027. doi: 10.1210/endo-92-4-1022. [DOI] [PubMed] [Google Scholar]
  6. Garnier J., Salesse R., Pernollet J. C. Reversible folding of human chorionic gonadotropin at acid pH or upon recombination of the alpha and beta subunits. FEBS Lett. 1974 Sep 1;45(1):166–171. doi: 10.1016/0014-5793(74)80837-9. [DOI] [PubMed] [Google Scholar]
  7. Hearn J. P. Immunization against pregnancy. Proc R Soc Lond B Biol Sci. 1976 Dec 10;195(1118):149–160. doi: 10.1098/rspb.1976.0105. [DOI] [PubMed] [Google Scholar]
  8. Ingham K. C., Tylenda C., Edelhoch H. Structural studies of human chorionic gonadotropin and its subunits using tyrosine fluorescence. Arch Biochem Biophys. 1976 Apr;173(2):680–690. doi: 10.1016/0003-9861(76)90306-4. [DOI] [PubMed] [Google Scholar]
  9. Interaction of 1,8-ANS with human luteinizing hormones: a probe for subunit interactions of hCG and hLH. Arch Biochem Biophys. 1973 Apr;155(2):478–479. doi: 10.1016/0003-9861(73)90141-0. [DOI] [PubMed] [Google Scholar]
  10. Kammerman S., Canfield R. E., Kolena J., Channing C. P. The binding of iodinated hCG to porcine granulosa cells. Endocrinology. 1972 Jul;91(1):65–74. doi: 10.1210/endo-91-1-65. [DOI] [PubMed] [Google Scholar]
  11. Lee C. Y., Ryan R. J. Interaction of ovarian receptors with human luteinizing hormone and human chorionic gonadotropin. Biochemistry. 1973 Nov 6;12(23):4609–4615. doi: 10.1021/bi00747a011. [DOI] [PubMed] [Google Scholar]
  12. Morgan F. J., Birken S., Canfield R. E. The amino acid sequence of human chorionic gonadotropin. The alpha subunit and beta subunit. J Biol Chem. 1975 Jul 10;250(13):5247–5258. [PubMed] [Google Scholar]
  13. Morgan F. J., Canfield R. E., Vaitukaitis J. L., Ross G. T. Properties of the subunits of human chorionic gonadotropin. Endocrinology. 1974 Jun;94(6):1601–1606. doi: 10.1210/endo-94-6-1601. [DOI] [PubMed] [Google Scholar]
  14. Muralidhar K., Moudgal N. R. Studies on rat ovarian receptors for lutropin (luteinizing hormone). Interaction with beta-subunit of sheep lutropin. Biochem J. 1976 Dec 15;160(3):615–619. doi: 10.1042/bj1600615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pernollet J. C., Garnier J., Pierce J. G., Salesse R. In vitro activation of glycoprotein hormones. Hybridization of subunits from thyrotropin, lutropin and human choriogonadotropin. Biochim Biophys Acta. 1976 Sep 28;446(1):262–276. doi: 10.1016/0005-2795(76)90117-3. [DOI] [PubMed] [Google Scholar]
  16. Pierce J. G., Bahl O. P., Cornell J. S., Swaminathan N. Biologically active hormones prepared by recombination of the alpha chain of human chorionic gonadotropin and the hormone-specific chain of bovine thyrotropin or of bovine luteinizing hormone. J Biol Chem. 1971 Apr 10;246(7):2321–2324. [PubMed] [Google Scholar]
  17. Pierce J. G., Faith M. R., Giudice L. C., Reeve J. R. Structure and structure-function relationships in glycoprotein hormones. Ciba Found Symp. 1976;41:225–250. doi: 10.1002/9780470720233.ch12. [DOI] [PubMed] [Google Scholar]
  18. Rao C. V., Carman F., Jr Stimulation of ovarian cyclic guanosine 3',5'-monophosphate levels by the subunits of human chorionic gonadotropin. Biochem Biophys Res Commun. 1973 Sep 18;54(2):744–750. doi: 10.1016/0006-291x(73)91486-1. [DOI] [PubMed] [Google Scholar]
  19. Rayford P. L., Vaitukaitis J. L., Ross G. T., Morgan F. J., Canfield R. E. Use of specific antisera to characterize biologic activity of hCG-beta subunit preparations. Endocrinology. 1972 Jul;91(1):144–146. doi: 10.1210/endo-91-1-144. [DOI] [PubMed] [Google Scholar]
  20. Reichert L. E., Jr, Trowbridge C. G., Bhalla V. K., Lawson G. M., Jr The kinetics of formation and biological activity of native and hybrid molecules of human follicle-stimulating hormone. J Biol Chem. 1974 Oct 25;249(20):6472–6477. [PubMed] [Google Scholar]
  21. Salesse R., Castaing M., Pernollet J. C., Garnier J. Association-dependent active folding of alpha and beta subunits of lutropin (luteinizing hormone). J Mol Biol. 1975 Jul 15;95(4):483–496. doi: 10.1016/0022-2836(75)90312-5. [DOI] [PubMed] [Google Scholar]
  22. Talwar G. P., Sharma N. C., Dubey S. K., Salahuddin M., Das C., Ramakrishnan S., Kumar S., Hingorani V. Isoimmunization against human chorionic gonadotropin with conjugates of processed beta-subunit of the hormone and tetanus toxoid. Proc Natl Acad Sci U S A. 1976 Jan;73(1):218–222. doi: 10.1073/pnas.73.1.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Van Damme M. P., Robertson D. M., Diczfalusy E. An improved in vitro bioassay method for measuring luteinizing hormone (LH) activity using mouse Leydig cell preparations. Acta Endocrinol (Copenh) 1974 Dec;77(4):655–671. doi: 10.1530/acta.0.0770655. [DOI] [PubMed] [Google Scholar]
  24. Yang W. H., Sairam M. R., Li C. H. The effect of ICSH- and its combination with prolactin on the maintenance of pregnancy in the rat. Acta Endocrinol (Copenh) 1973 Jan;72(1):173–181. doi: 10.1530/acta.0.0720173. [DOI] [PubMed] [Google Scholar]
  25. Yang W. H., Sairam M. R., Papkoff H., Li C. H. Ovulation in hamster: induction by subunit of ovine interstitial cell stimulating hormone. Science. 1972 Feb 11;175(4022):637–638. doi: 10.1126/science.175.4022.637. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES