Abstract
Incorporation of D-3-hydroxy[3-14C]butyrate into lipid in vivo suggests that lactating mammary gland is a major site of ketone-body utilization. The incorporation decreases in short-term insulin deficiency (2h) and on starvation (24h), but increases again on refeeding (2h). The activity of cytosolic acetoacetyl-CoA synthetase parallels the changes in nutritional state, but is not affected by short-term insulin deficiency.
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Selected References
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- Bartley J. C. Modulation by ketone bodies of the rate of fatty acid synthesis in mammary gland slices from lactating rats. Lipids. 1976 Oct;11(10):774–777. doi: 10.1007/BF02533054. [DOI] [PubMed] [Google Scholar]
- Bates M. W., Krebs H. A., Williamson D. H. Turnover rates of ketone bodies in normal, starved and alloxan-diabetic rats. Biochem J. 1968 Dec;110(4):655–661. doi: 10.1042/bj1100655. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Buckley B. M., Williamson D. H. Acetoacetate and brain lipogenesis: developmental pattern of acetoacetyl-coenzyme A synthetase in the soluble fraction of rat brain. Biochem J. 1973 Mar;132(3):653–656. doi: 10.1042/bj1320653. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Buckley B. M., Williamson D. H. Acetoacetyl-CoA synthetase; a lipogenic enzyme in rat tissues. FEBS Lett. 1975 Dec 1;60(1):7–10. doi: 10.1016/0014-5793(75)80406-6. [DOI] [PubMed] [Google Scholar]
- Chatwin A. L., Linzell J. L., Setchell B. P. Cardiovascular changes during lactation in the rat. J Endocrinol. 1969 Jun;44(2):247–254. doi: 10.1677/joe.0.0440247. [DOI] [PubMed] [Google Scholar]
- GOERANSSON G., OLIVECRONA T. THE METABOLISM OF FATTY ACIDS IN THE RAT. I. PALMITIC ACID. Acta Physiol Scand. 1964 Nov;62:224–239. doi: 10.1111/j.1748-1716.1964.tb03970.x. [DOI] [PubMed] [Google Scholar]
- Kinsella J. E. Biosynthesis of lipids from (2-14C)acetate and D(-)-beta-hydroxy(1,3-14C)butyrate by mammary cells from bovine and rat. Biochim Biophys Acta. 1970 Jun 9;210(1):28–38. doi: 10.1016/0005-2760(70)90058-5. [DOI] [PubMed] [Google Scholar]
- LAURELL S. Recycling of intravenously injected palmitic acid-1-C14 as esterified fatty acid in the plasma of rats and turnover rate of plasma triglycerides. Acta Physiol Scand. 1959 Nov 15;47:218–232. doi: 10.1111/j.1748-1716.1960.tb00072.x. [DOI] [PubMed] [Google Scholar]
- Page M. A., Williamson D. H. Lactating mammary gland of the rat: a potential major site of ketone-body utilization. Biochem J. 1972 Jun;128(2):459–460. doi: 10.1042/bj1280459. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robinson A. M., Girard J. R., Williamson D. H. Evidence for a role of insulin in the regulation of lipogenesis in lactating rat mammary gland. Measurements of lipogenesis in vivo and plasma hormone concentrations in response to starvation and refeeding. Biochem J. 1978 Oct 15;176(1):343–346. doi: 10.1042/bj1760343. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robinson A. M., Williamson D. H. Comparison of glucose metabolism in the lactating mammary gland of the rat in vivo and in vitro. Effects of starvation, prolactin or insulin deficiency. Biochem J. 1977 Apr 15;164(1):153–159. doi: 10.1042/bj1640153. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robinson A. M., Williamson D. H. Control of glucose metabolism in isolated acini of the lactating mammary gland of the rat. The ability of glycerol to mimic some of the effects of insulin. Biochem J. 1977 Dec 15;168(3):465–474. doi: 10.1042/bj1680465. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robinson A. M., Williamson D. H. Effects of acetoacetate administration on glucose metabolism in mammary gland of fed lactating rats. Biochem J. 1977 Jun 15;164(3):749–752. doi: 10.1042/bj1640749. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rous S. Fasting and insulin regulation of the utlization of acetoacetate for fatty acid synthesis. Arch Biochem Biophys. 1977 Feb;179(1):328–333. doi: 10.1016/0003-9861(77)90118-7. [DOI] [PubMed] [Google Scholar]
- Stansbie D., Brownsey R. W., Crettaz M., Denton R. M. Acute effects in vivo of anti-insulin serum on rates of fatty acid synthesis and activities of acetyl-coenzyme A carboxylase and pyruvate dehydrogenase in liver and epididymal adipose tissue of fed rats. Biochem J. 1976 Nov 15;160(2):413–416. doi: 10.1042/bj1600413. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stern J. R. A role of acetoacetyl-CoA synthetase in acetoacetate utilization by rat liver cell fractions. Biochem Biophys Res Commun. 1971 Aug 20;44(4):1001–1007. doi: 10.1016/0006-291x(71)90811-4. [DOI] [PubMed] [Google Scholar]
- WILLIAMSON D. H., MELLANBY J., KREBS H. A. Enzymic determination of D(-)-beta-hydroxybutyric acid and acetoacetic acid in blood. Biochem J. 1962 Jan;82:90–96. doi: 10.1042/bj0820090. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Whitelaw E., Williamson D. H. Effects of lactation of ketogenesis from oleate or butyrate in rat hepatocytes. Biochem J. 1977 Jun 15;164(3):521–528. doi: 10.1042/bj1640521. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Williamson D. H., McKeown S. R., Ilic V. Interactions of glucose, acetoacetate and insulin in mammary-gland slices of lactating rats. Biochem J. 1975 Aug;150(2):145–152. doi: 10.1042/bj1500145. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wolczunowicz M., Rous S. Regulation of cytoplasmic acetyl-CoA and acetoacetyl-CoA synthetases by dexamethasone phosphate in rat liver and adipose tissue. Life Sci. 1977 Apr 15;20(8):1347–1352. doi: 10.1016/0024-3205(77)90360-5. [DOI] [PubMed] [Google Scholar]
