Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1978 Dec 15;176(3):1007–1010. doi: 10.1042/bj1761007

Thyroid-hormone modulation of the number of beta-adrenergic receptors in rat fat-cell membranes.

Y Giudicelli
PMCID: PMC1186327  PMID: 218550

Abstract

Adipocytes from thyroidectomized rats contain 3 times less [3H]dihydroalprenolol-binding sites (beta-adrenergic receptors) than adipocytes from euthyroid animals. This alteration is not solely due to cell-size differences, but also to a thyroidectomy-induced defect in beta-adrenergic receptor density per adipocyte surface area, a defect that is furthermore corrected by tri-iodothyronine treatment.

Full text

PDF
1007

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander R. W., Williams L. T., Lefkowitz R. J. Identification of cardiac beta-adrenergic receptors by (minus) [3H]alprenolol binding. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1564–1568. doi: 10.1073/pnas.72.4.1564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Armstrong K. J., Stouffer J. E., Van Inwegen R. G., Thompson W. J., Robison G. A. Effects of thyroid hormone deficiency on cyclic adenosine 3':5'-monophosphate and control of lipolysis in fat cells. J Biol Chem. 1974 Jul 10;249(13):4226–4231. [PubMed] [Google Scholar]
  3. Avruch J., Wallach D. F. Preparation and properties of plasma membrane and endoplasmic reticulum fragments from isolated rat fat cells. Biochim Biophys Acta. 1971 Apr 13;233(2):334–347. doi: 10.1016/0005-2736(71)90331-2. [DOI] [PubMed] [Google Scholar]
  4. Banerjee S. P., Kung L. S. beta-Adrenergic receptors in rat heart: effects of thyroidectomy. Eur J Pharmacol. 1977 May 15;43(2):207–208. doi: 10.1016/0014-2999(77)90134-0. [DOI] [PubMed] [Google Scholar]
  5. Ciaraldi T. P., Marinetti G. V. Hormone action at the membrane level. VIII. Adrenergic receptors in rat heart and adipocytes and their modulation by thyroxine. Biochim Biophys Acta. 1978 Jul 3;541(3):334–346. doi: 10.1016/0304-4165(78)90193-9. [DOI] [PubMed] [Google Scholar]
  6. Correze C., Laudat M. H., Laudat P., Nunez J. Hormone-dependent lipolysis in fat-cells from thyroidectomized rats. Mol Cell Endocrinol. 1974 Oct;1(5):309–327. doi: 10.1016/0303-7207(74)90021-5. [DOI] [PubMed] [Google Scholar]
  7. Giudicelli Y., Provin D., Nordmann R. Influence of various inhibitors of glycolysis and oxidative phosphorylation on the lipolytic activity of isolated human adipocytes. Biochimie. 1975;57(8):979–981. doi: 10.1016/s0300-9084(75)80222-7. [DOI] [PubMed] [Google Scholar]
  8. Giudicelli Y., Provin D., Pecquery R., Nordmann R. Influence of trypsin on lipolysis in human fat cells. Comparison with rat adipocytes. Biochim Biophys Acta. 1976 Dec 20;450(3):358–366. doi: 10.1016/0005-2760(76)90009-6. [DOI] [PubMed] [Google Scholar]
  9. Goldrick R. B. Morphological changes in the adipocyte during fat deposition and mobilization. Am J Physiol. 1967 Apr;212(4):777–782. doi: 10.1152/ajplegacy.1967.212.4.777. [DOI] [PubMed] [Google Scholar]
  10. Hirsch J., Gallian E. Methods for the determination of adipose cell size in man and animals. J Lipid Res. 1968 Jan;9(1):110–119. [PubMed] [Google Scholar]
  11. Kempson S., Marinetti G. V., Shaw A. Hormone action at the membrane level. VII. Stimulation of dihydroalprenolol binding to beta-adrenergic receptors in isolated rat heart ventricle slices by triiodothyronine and thyroxine. Biochim Biophys Acta. 1978 May 3;540(2):320–329. doi: 10.1016/0304-4165(78)90145-9. [DOI] [PubMed] [Google Scholar]
  12. Krishna G., Hynie S., Brodie B. B. Effects of thyroid hormones on adenyl cyclase in adipose tissue and on free fatty acid mobilization. Proc Natl Acad Sci U S A. 1968 Mar;59(3):884–889. doi: 10.1073/pnas.59.3.884. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Lo S. C., August T. R., Liberman U. A., Edelman I. S. Dependence of renal (Na+ + k+)-adenosine triphosphatase activity on thyroid status. J Biol Chem. 1976 Dec 25;251(24):7826–7833. [PubMed] [Google Scholar]
  15. Malbon C. C., Moreno F. J., Cabelli R. J., Fain J. N. Fat cell adenylate cyclase and beta-adrenergic receptors in altered thyroid states. J Biol Chem. 1978 Feb 10;253(3):671–678. [PubMed] [Google Scholar]
  16. Mukherjee C., Caron M. G., Coverstone M., Lefkowitz R. J. Identification of adenylate cyclase-coupled beta-adrenergic receptors in frog erythrocytes with (minus)-[3-H] alprenolol. J Biol Chem. 1975 Jul 10;250(13):4869–4876. [PubMed] [Google Scholar]
  17. RODBELL M. METABOLISM OF ISOLATED FAT CELLS. I. EFFECTS OF HORMONES ON GLUCOSE METABOLISM AND LIPOLYSIS. J Biol Chem. 1964 Feb;239:375–380. [PubMed] [Google Scholar]
  18. Stoffer S. S., Jiang N. S., Gorman C. A., Pikler G. M. Plasma catecholamines in hypothyroidism and hyperthyroidism. J Clin Endocrinol Metab. 1973 Mar;36(3):587–589. doi: 10.1210/jcem-36-3-587. [DOI] [PubMed] [Google Scholar]
  19. Waldstein S. S. Thyroid-catecholamine interrelations. Annu Rev Med. 1966;17:123–132. doi: 10.1146/annurev.me.17.020166.001011. [DOI] [PubMed] [Google Scholar]
  20. Williams L. T., Jarett L., Lefkowitz R. J. Adipocyte beta-adrenergic receptors. Identification and subcellular localization by (-)-[3H]dihydroalprenolol binding. J Biol Chem. 1976 May 25;251(10):3096–3104. [PubMed] [Google Scholar]
  21. Williams L. T., Lefkowitz R. J., Watanabe A. M., Hathaway D. R., Besch H. R., Jr Thyroid hormone regulation of beta-adrenergic receptor number. J Biol Chem. 1977 Apr 25;252(8):2787–2789. [PubMed] [Google Scholar]
  22. Williams L. T., Snyderman R., Lefkowitz R. J. Identification of beta-adrenergic receptors in human lymphocytes by (-) (3H) alprenolol binding. J Clin Invest. 1976 Jan;57(1):149–155. doi: 10.1172/JCI108254. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES