Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1979 Jan 1;177(1):251–254. doi: 10.1042/bj1770251

Partial non-cleavage by cyanogen bromide of a methionine--cystine bond from human serum albumin and bovine alpha-lactalbumin.

N Doyen, C Lapresle
PMCID: PMC1186363  PMID: 426771

Abstract

When human albumin was treated with CNBr, a fragment designated D was obtained and attributed to the absence from some of the albumin molecules of methionine at position 123 [Lapresle & Doyen (1975) Biochem. J. 151, 637-643]. The present study shows that methionine-123 is converted into homoserine without cleavage of the subsequent methionine-cystine bond. With bovine alpha-lactalbumin, a further example of non-cleavage of a methionine-cystine bond with conversion of methionine into homoserine is reported.

Full text

PDF

Images in this article

252Fig. 1.
on p.252

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brew K., Castellino F. J., Vanaman T. C., Hill R. L. The complete amino acid sequence of bovine alpha-lactalbumin. J Biol Chem. 1970 Sep 10;245(17):4570–4582. [PubMed] [Google Scholar]
  2. Brew K., Hill R. L. The isolation and characterization of the tryptic, chymotryptic, peptic, and cyanogen bromide peptides from bovine alpha-lactalbumin. J Biol Chem. 1970 Sep 10;245(17):4559–4569. [PubMed] [Google Scholar]
  3. Carpenter F. H., Shiigi S. M. Cyanogen bromide treatment of N-acetylmethionyl residues without cleavage. Biochemistry. 1974 Dec 3;13(25):5159–5164. doi: 10.1021/bi00722a017. [DOI] [PubMed] [Google Scholar]
  4. Corradin G., Harbury H. A. Cleavage of cytochrome c with cyanogen bromide. Biochim Biophys Acta. 1970 Dec 22;221(3):489–496. doi: 10.1016/0005-2795(70)90219-9. [DOI] [PubMed] [Google Scholar]
  5. Cummingham B. A., Gottlieb P. D., Konigsberg W. H., Edelman G. M. The covalent structure of a human gamma G-immunoglobulin. V. Partial amino acid sequence of the light chain. Biochemistry. 1968 May;7(5):1983–1994. doi: 10.1021/bi00845a049. [DOI] [PubMed] [Google Scholar]
  6. DeLange R. J. Egg white avidin. I. Amino acid composition; sequence of the amino- and carboxyl-terminal cyanogen bromide peptides. J Biol Chem. 1970 Mar 10;245(5):907–916. [PubMed] [Google Scholar]
  7. Lapresle C., Doyen N. Isolation and properties of a fragment of human serum albumin demonstrating the absence of a methionine residue from some of the albumin molecules. Biochem J. 1975 Dec;151(3):637–643. doi: 10.1042/bj1510637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McMenamy R. H., Dintzis H. M., Watson F. Cyanogen bromide fragments of human serum albumin. J Biol Chem. 1971 Aug 10;246(15):4744–4750. [PubMed] [Google Scholar]
  9. Meloun B., Morávek L., Kostka V. Complete amino acid sequence of human serum albumin. FEBS Lett. 1975 Oct 15;58(1):134–137. doi: 10.1016/0014-5793(75)80242-0. [DOI] [PubMed] [Google Scholar]
  10. Narita K., Chitani K. The amino acid sequence of cytochrome C from Candida krusei. J Biochem. 1968 Feb;63(2):226–241. doi: 10.1093/oxfordjournals.jbchem.a128765. [DOI] [PubMed] [Google Scholar]
  11. Saunders D. J., Offord R. Semisynthetic analogues of insulin. The use of N-substituted derivatives of methionine as acid-stable protecting groups. Biochem J. 1977 Sep 1;165(3):479–486. doi: 10.1042/bj1650479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schroeder W. A., Shelton J. B., Shelton J. R. An examination of conditions for the cleavage of polypeptide chains with cyanogen bromide: application to catalase. Arch Biochem Biophys. 1969 Mar;130(1):551–556. doi: 10.1016/0003-9861(69)90069-1. [DOI] [PubMed] [Google Scholar]
  13. Uriel J. Méthode d'électrophorèse dans des gels d'acrylamide-agarose. Bull Soc Chim Biol (Paris) 1966;48(8):969–982. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES