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. 1979 Feb 1;177(2):765–768. doi: 10.1042/bj1770765

Mapping the membrane proteins of Newcastle-disease virus with a photoreactive glycolipid probe.

J S Bramhall, M A Shiflett, B J Wisnieski
PMCID: PMC1186431  PMID: 435268

Abstract

The envelope proteins of Newcastle-disease virus were preferentially labelled when a suspension of virus particles that contained the photoreactive probe 12-(4-azido-2-nitro-phenoxy)stearoyl[1-14C]glucosamine was irradiated. One of the proteins labelled was not readily accessible to surface labelling with 125I.

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Selected References

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  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Greenberg G. R., Chakrabarti P., Khorana H. G. Incorporation of fatty acids containing photosensitive groups into phospholipids of Escherichia coli. Proc Natl Acad Sci U S A. 1976 Jan;73(1):86–90. doi: 10.1073/pnas.73.1.86. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Iwata K. K., Manweiler C. A., Bramhall J., Wisnieski B. J. Photoreactive probes for high resolution mapping of membrane proteins. Prog Clin Biol Res. 1978;22:579–589. [PubMed] [Google Scholar]
  4. Lapidot Y., Rappoport S., Wolman Y. Use of esters of N-hydroxysuccinimide in the synthesis of N-acylamino acids. J Lipid Res. 1967 Mar;8(2):142–145. [PubMed] [Google Scholar]
  5. Lenard J. Virus envelopes and plasma membranes. Annu Rev Biophys Bioeng. 1978;7:139–165. doi: 10.1146/annurev.bb.07.060178.001035. [DOI] [PubMed] [Google Scholar]
  6. Marchalonis J. J. An enzymic method for the trace iodination of immunoglobulins and other proteins. Biochem J. 1969 Jun;113(2):299–305. doi: 10.1042/bj1130299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rifkin D. B., Compans R. W., Reich E. A specific labeling procedure for proteins on the outer surface of membranes. J Biol Chem. 1972 Oct 25;247(20):6432–6437. [PubMed] [Google Scholar]
  8. Stoffel W., Därr W., Salm K. P. Chemical proof of lipid-protein interactions by crosslinking photosensitive lipids to apoproteins. Intermolecular cross-linkage between high-density apolipoprotein A-I and lecithins and sphingomyelins. Hoppe Seylers Z Physiol Chem. 1977 Apr;358(4):453–462. doi: 10.1515/bchm2.1977.358.1.453. [DOI] [PubMed] [Google Scholar]
  9. Wisnieski B. J., Iwata K. K. Electron spin resonance evidence for vertical asymmetry in animal cell membranes. Biochemistry. 1977 Apr 5;16(7):1321–1326. doi: 10.1021/bi00626a013. [DOI] [PubMed] [Google Scholar]

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