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. 1979 Mar 1;177(3):847–852. doi: 10.1042/bj1770847

Hydroxylysine-linked glycosides of human complement subcomponent C1q and various collagens.

H Shinkai, K Yonemasu
PMCID: PMC1186449  PMID: 444207

Abstract

1. Human C1q, a subcomponent of the first component of complement, contains 67 disaccharides (glucosylgalactose) and 2.4 monosaccharides (galactose) linked to hydroxylysine in one molecule. It was found that 82.6% of the hydroxylsine residues were glycosylated. The suggestion of the possible existence of glucosylgalactosylhydroxylysine reported previously [Yonemasu, Stroud, Niedermeir & Butler (1971) Biochem. Biophys. Res. Commun. 43, 1388--1394] was confirmed. 2. The hydroxylysine-glycosides are not detected in the C-terminal, non-collagen-like, globular regions, but only in the collagen-like regions in the subcomponent C1q molecule. 3. Alpha 1(I) and alpha 2 in pig skin, alpha 1(II) in bovine cartilage and alpha 1(III) in bovine skin collagens contain 2.0, 2.2, 13.2 and 2.0 residues of hydroxylysine-glycosides per molecule, respectively. The percentage of hydroxylysine residues glycosylated in each of these chains is relatively low (on average 38%). 4. Neither the high percentage of hydroxylysine residues glycosylated nor the high values for the ratios of disaccharides to monosaccharides in the subcomponent C1q resembles that in alpha 1(I), alpha 2, alpha 1(II) and alpha 1(III). 5. Similarities between the extent of glycosylation of hydroxylysine residues in collagen-like regions in the subcomponent C1q molecule and that of the collagenous constituents of human glomerular basement membranes, aortic intima, skin A- and B-chains and of bovine anterior lens capsule are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Calcott M. A., Müller-Eberhard H. J. C1q protein of human complement. Biochemistry. 1972 Aug 29;11(18):3443–3450. doi: 10.1021/bi00768a018. [DOI] [PubMed] [Google Scholar]
  2. Chung E., Miller E. J. Collagen polymorphism: characterization of molecules with the chain composition (alpha 1 (3)03 in human tissues. Science. 1974 Mar;183(130):1200–1201. doi: 10.1126/science.183.4130.1200. [DOI] [PubMed] [Google Scholar]
  3. Chung E., Rhodes K., Miller E. J. Isolation of three collagenous components of probable basement membrane origin from several tissues. Biochem Biophys Res Commun. 1976 Aug 23;71(4):1167–1174. doi: 10.1016/0006-291x(76)90776-2. [DOI] [PubMed] [Google Scholar]
  4. Dixit S. N. Isolation and characterization of two collagenous components from anterior lens capsule. FEBS Lett. 1978 Jan 1;85(1):153–157. doi: 10.1016/0014-5793(78)81269-1. [DOI] [PubMed] [Google Scholar]
  5. Fujii T., Kühn K. Isolation and characterization of pepsin-treated type III collagen from calf skin. Hoppe Seylers Z Physiol Chem. 1975 Nov;356(11):1793–1801. doi: 10.1515/bchm2.1975.356.2.1793. [DOI] [PubMed] [Google Scholar]
  6. Kefalides N. A. Basement membranes: current concepts of structure and synthesis. Dermatologica. 1975;150(1):4–15. doi: 10.1159/000251384. [DOI] [PubMed] [Google Scholar]
  7. Matsubayashi S., Shinkai H., Sano S. Characterization of the synthesized collagen by mouse L-929 (L-5) cells. Arch Dermatol Res. 1977 Oct 27;260(1):7–15. doi: 10.1007/BF00558009. [DOI] [PubMed] [Google Scholar]
  8. Miller E. J. Structural studies on cartilage collagen employing limited cleavage and solubilization with pepsin. Biochemistry. 1972 Dec 19;11(26):4903–4909. doi: 10.1021/bi00776a005. [DOI] [PubMed] [Google Scholar]
  9. Mizuochi T., Yonemasu K., Yamashita K., Kobata A. The asparagine-linked sugar chains of subcomponent C1q of the first component of human complement. J Biol Chem. 1978 Oct 25;253(20):7404–7409. [PubMed] [Google Scholar]
  10. Porter W. H. Application of nitrous acid deamination of hexosamines to the simultaneous GLC determination of neutral and amino sugars in glycoproteins. Anal Biochem. 1975 Jan;63(1):27–43. doi: 10.1016/0003-2697(75)90186-4. [DOI] [PubMed] [Google Scholar]
  11. Reid K. B. A collagen-like amino acid sequence in a polypeptide chain of human C1q (a subcomponent of the first component of complement). Biochem J. 1974 Jul;141(1):189–203. doi: 10.1042/bj1410189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Reid K. B. Amino acid sequence of the N-terminal forty-two amino acid residues of the C chain of subcomponent C1q of the first component of human complement. Biochem J. 1977 Feb 1;161(2):247–251. doi: 10.1042/bj1610247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Reid K. B. Isolation, by partial pepsin digestion, of the three collagen-like regions present in subcomponent Clq of the first component of human complement. Biochem J. 1976 Apr 1;155(1):5–17. doi: 10.1042/bj1550005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Reid K. B., Lowe D. M., Porter R. R. Isolation and characterization of C1q, a subcomponent of the first component of complement, from human and rabbit sera. Biochem J. 1972 Dec;130(3):749–763. doi: 10.1042/bj1300749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Reid K. B., Porter R. R. Subunit composition and structure of subcomponent C1q of the first component of human complement. Biochem J. 1976 Apr 1;155(1):19–23. doi: 10.1042/bj1550019. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Reid K. B., Thompson E. O. Amino acid sequence of the N-terminal 108 amino acid residues of the B chain of subcomponent C1q of the first component of human complement. Biochem J. 1978 Sep 1;173(3):863–868. doi: 10.1042/bj1730863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Spiro R. G. The structure of the disaccharide unit of the renal glomerular basement membrane. J Biol Chem. 1967 Oct 25;242(20):4813–4823. [PubMed] [Google Scholar]
  18. Strawich E., Nimni M. E. Properties of a collagen molecule containing three identical components extracted from bovine articular cartilage. Biochemistry. 1971 Oct 12;10(21):3905–3911. doi: 10.1021/bi00797a017. [DOI] [PubMed] [Google Scholar]
  19. Yonemasu K., Stroud R. M. Clq: rapid purification method for preparation of monospecific antisera and for biochemical studies. J Immunol. 1971 Feb;106(2):304–313. [PubMed] [Google Scholar]
  20. Yonemasu K., Stroud R. M., Niedermeier W., Butler W. T. Chemical studies of Clq; a modulator of immunoglobulin biology. Biochem Biophys Res Commun. 1971 Jun 18;43(6):1388–1394. doi: 10.1016/s0006-291x(71)80028-1. [DOI] [PubMed] [Google Scholar]
  21. Yonemasu K., Stroud R. M. Structural studies on human Clq: non-covalent and covalent subunits. Immunochemistry. 1972 May;9(5):545–554. doi: 10.1016/0019-2791(72)90064-x. [DOI] [PubMed] [Google Scholar]

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