Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1979 Mar 1;177(3):989–991. doi: 10.1042/bj1770989

Cholesterol exchange as a function of cholesterol/phospholipid mole ratios.

M J Poznansky, S Czekanski
PMCID: PMC1186468  PMID: 444215

Abstract

The activation energy (Ea) for cholesterol exchange between dioleoyl phosphatidylcholine vesicles and erythrocyte 'ghosts' is measured as a function of molar percentage of cholesterol in both donor and acceptor membranes. A sharp increase in Ea occurs (from 39.9kJ/mol to 84kJ/mol) when the molar percentage of cholesterol decreases from 30 to 20%.

Full text

PDF
989

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cubero Robles E., van den Berg D. Synthesis of lecithins by acylation of O-(sn-glycero-3-phosphoryl) choline with fatty acid anhydrides. Biochim Biophys Acta. 1969 Dec 17;187(4):520–526. doi: 10.1016/0005-2760(69)90049-6. [DOI] [PubMed] [Google Scholar]
  2. DODGE J. T., MITCHELL C., HANAHAN D. J. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch Biochem Biophys. 1963 Jan;100:119–130. doi: 10.1016/0003-9861(63)90042-0. [DOI] [PubMed] [Google Scholar]
  3. De Kruyff B., Van Dijck P. W., Demel R. A., Schuijff A., Brants F., Van Deenen L. L. Non-random distribution of cholesterol in phosphatidylcholine bilayers. Biochim Biophys Acta. 1974 Jul 12;356(1):1–7. doi: 10.1016/0005-2736(74)90288-0. [DOI] [PubMed] [Google Scholar]
  4. Demel R. A., De Kruyff B. The function of sterols in membranes. Biochim Biophys Acta. 1976 Oct 26;457(2):109–132. doi: 10.1016/0304-4157(76)90008-3. [DOI] [PubMed] [Google Scholar]
  5. Engelman D. M., Rothman J. E. The planar organization of lecithin-cholesterol bilayers. J Biol Chem. 1972 Jun 10;247(11):3694–3697. [PubMed] [Google Scholar]
  6. Hinz H. J., Sturtevant J. M. Calorimetric investigation of the influence of cholesterol on the transition properties of bilayers formed from synthetic L- -lecithins in aqueous suspension. J Biol Chem. 1972 Jun 10;247(11):3697–3700. [PubMed] [Google Scholar]
  7. Huang C. Studies on phosphatidylcholine vesicles. Formation and physical characteristics. Biochemistry. 1969 Jan;8(1):344–352. doi: 10.1021/bi00829a048. [DOI] [PubMed] [Google Scholar]
  8. Kleemann W., McConnell H. M. Interactions of proteins and cholesterol with lipids in bilayer membranes. Biochim Biophys Acta. 1976 Jan 21;419(2):206–222. doi: 10.1016/0005-2736(76)90347-3. [DOI] [PubMed] [Google Scholar]
  9. Ladbrooke B. D., Williams R. M., Chapman D. Studies on lecithin-cholesterol-water interactions by differential scanning calorimetry and X-ray diffraction. Biochim Biophys Acta. 1968 Apr 29;150(3):333–340. doi: 10.1016/0005-2736(68)90132-6. [DOI] [PubMed] [Google Scholar]
  10. Mabrey S., Mateo P. L., Sturtevant J. M. High-sensitivity scanning calorimetric study of mixtures of cholesterol with dimyristoyl- and dipalmitoylphosphatidylcholines. Biochemistry. 1978 Jun 13;17(12):2464–2468. doi: 10.1021/bi00605a034. [DOI] [PubMed] [Google Scholar]
  11. Oldfield E., Chapman D. Dynamics of lipids in membranes: Heterogeneity and the role of cholesterol. FEBS Lett. 1972 Jul 1;23(3):285–297. doi: 10.1016/0014-5793(72)80300-4. [DOI] [PubMed] [Google Scholar]
  12. Poznansky M. J., Lange Y. Transbilayer movement of cholesterol in phospholipid vesicles under equilibrium and non-equilibrium conditions. Biochim Biophys Acta. 1978 Jan 19;506(2):256–264. doi: 10.1016/0005-2736(78)90396-6. [DOI] [PubMed] [Google Scholar]
  13. Rothman J. E., Engelman D. M. Molecular mechanism for the interaction of phospholipid with cholesterol. Nat New Biol. 1972 May 10;237(71):42–44. doi: 10.1038/newbio237042a0. [DOI] [PubMed] [Google Scholar]
  14. Shimshick E. J., McConnell H. M. Lateral phase separations in binary mixtures of cholesterol and phospholipids. Biochem Biophys Res Commun. 1973 Jul 17;53(2):446–451. doi: 10.1016/0006-291x(73)90682-7. [DOI] [PubMed] [Google Scholar]
  15. Van Dijck P. W., De Kruijff B., Van Deenen L. L., De Gier J., Demel R. A. The preference of cholesterol for phosphatidylcholine in mixed phosphatidylcholine-phosphatidylethanolamine bilayers. Biochim Biophys Acta. 1976 Dec 2;455(2):576–587. doi: 10.1016/0005-2736(76)90326-6. [DOI] [PubMed] [Google Scholar]
  16. de Kruijff B. 13C NMR studies on [4-13C] cholesterol incorporated in sonicated phosphatidylcholine vesicles. Biochim Biophys Acta. 1978 Jan 19;506(2):173–182. doi: 10.1016/0005-2736(78)90388-7. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES