Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1979 Feb 15;178(2):345–352. doi: 10.1042/bj1780345

Distinction between progestin- and glucocorticoid-binding sites in mammary glands. Apparent lack of cytoplasmic progesterone receptors in lactating mammary glands.

G Shyamala, W A McBlain
PMCID: PMC1186521  PMID: 571720

Abstract

The cytosol fraction of the lactating mammary glands of mice does not appear to contain detectable amounts of progesterone receptors. Mixing experiments indicate that the absence of receptors is not due to interference by other factors in the cytosol. However, in the cytosol of mammary glands, there is specific binding or progestins to certain low-affinity sites which have characteristics of specific glucocorticoid-binding sites.

Full text

PDF
345

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asselin J., Labrie F., Kelly P. A., Philibert D., Raynaud J. P. Specific progesterone receptors in dimethylbenzanthracene (DMBA)-induced mammary tumors. Steroids. 1976 Mar;27(3):395–404. doi: 10.1016/0039-128x(76)90059-3. [DOI] [PubMed] [Google Scholar]
  2. BRESCIANI F. EFFECT OF OVARIAN HORMONES ON DURATION OF DNA SYNTHESIS IN CELLS OF THE C3H MOUSE MAMMARY GLAND. Exp Cell Res. 1965 Apr;38:13–32. doi: 10.1016/0014-4827(65)90423-4. [DOI] [PubMed] [Google Scholar]
  3. Baulieu E. E., Raynaud J. P., Milgrom E. Measurement of steroid binding proteins. Acta Endocrinol Suppl (Copenh) 1970;147:104–121. doi: 10.1530/acta.0.065s104. [DOI] [PubMed] [Google Scholar]
  4. Davis J. W., Wikman-Coffelt J., Eddington C. L. The effect of progesterone on biosynthetic pathways in mammary tissue. Endocrinology. 1972 Oct;91(4):1011–1019. doi: 10.1210/endo-91-4-1011. [DOI] [PubMed] [Google Scholar]
  5. Denamur R., Delouis C. Effects of progesterone and prolactin of the secretory activity and the nucleic acid content of the mammary gland of pregnant rabbits. Acta Endocrinol (Copenh) 1972 Jul;70(3):603–618. doi: 10.1530/acta.0.0700603. [DOI] [PubMed] [Google Scholar]
  6. DiSorbo D., Rosen F., McPartland R. P., Milholland R. J. Glucocorticoid activity of various progesterone analogs: correlation between specific binding in thymus and liver and biologic activity. Ann N Y Acad Sci. 1977 Mar 11;286:355–368. doi: 10.1111/j.1749-6632.1977.tb29429.x. [DOI] [PubMed] [Google Scholar]
  7. Feil P. D., Glasser S. R., Toft D. O., O'Malley B. W. Progesterone binding in the mouse and rat uterus. Endocrinology. 1972 Sep;91(3):738–746. doi: 10.1210/endo-91-3-738. [DOI] [PubMed] [Google Scholar]
  8. Feil P. D., Miljković M., Bardin C. W. Medroxyprogesterone acetate: a steroid with potent progestational activity but low receptor affinity in the guinea pig uterus. Endocrinology. 1976 Jun;98(6):1508–1515. doi: 10.1210/endo-98-6-1508. [DOI] [PubMed] [Google Scholar]
  9. Gardner D. G., Wittliff J. L. Characterization of a distinct glucocorticoid-binding protein in the lactating mammary gland of the rat. Biochim Biophys Acta. 1973 Oct 5;320(3):617–627. doi: 10.1016/0304-4165(73)90141-4. [DOI] [PubMed] [Google Scholar]
  10. Gardner D. G., Wittliff J. L. Specific estrogen receptors in the lactating mammary gland of the rat. Biochemistry. 1973 Jul 31;12(16):3090–3096. doi: 10.1021/bi00740a023. [DOI] [PubMed] [Google Scholar]
  11. Horwitz K. B., McGuire W. L. Progesterone and progesterone receptors in experimental breast cancer. Cancer Res. 1977 Jun;37(6):1733–1738. [PubMed] [Google Scholar]
  12. Kuhn N. J. Progesterone withdrawal as the lactogenic trigger in the rat. J Endocrinol. 1969 May;44(1):39–54. doi: 10.1677/joe.0.0440039. [DOI] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. LYONS W. R., LI C. H., JOHNSON R. E. The hormonal control of mammary growth and lactation. Recent Prog Horm Res. 1958;14:219–254. [PubMed] [Google Scholar]
  15. Leavitt W. W., Toft D. O., Strott C. A., O'Malley B. W. A specific progesterone receptor in the hamster uterus: physiologic properties and regulation during the estrous cycle. Endocrinology. 1974 Apr;94(4):1041–1053. doi: 10.1210/endo-94-4-1041. [DOI] [PubMed] [Google Scholar]
  16. MUNFORD R. E. CHANGES IN THE MAMMARY GLANDS OF RATS AND MICE DURING PREGNANCY, LACTATION AND INVOLUTION. 2. LEVELS OF DEOXYRIBONUCLEIC ACID, AND ALKALINE AND ACID PHOSPHATASES. J Endocrinol. 1963 Dec;28:17–34. doi: 10.1677/joe.0.0280017. [DOI] [PubMed] [Google Scholar]
  17. Matusik R. J., Rosen J. M. Prolactin induction of casein mRNA in organ culture. A model system for studying peptide hormone regulation of gene expression. J Biol Chem. 1978 Apr 10;253(7):2343–2347. [PubMed] [Google Scholar]
  18. McGuire J. L., Bariso C. D. Isolation and preliminary characterization of a progestogen specific binding macromolecule from the 273,000 g supernatant of rat and rabbit uteri. Endocrinology. 1972 Feb;90(2):496–506. doi: 10.1210/endo-90-2-496. [DOI] [PubMed] [Google Scholar]
  19. Milgrom E., Atger M., Baulieu E. E. Progesterone in uterus and plasma. IV. Progesterone receptor(s) in guinea pig uterus cytosol. Steroids. 1970 Dec;16(6):741–754. doi: 10.1016/s0039-128x(70)80152-0. [DOI] [PubMed] [Google Scholar]
  20. Milgrom E., Thi L., Atger M., Baulieu E. E. Mechanisms regulating the concentration and the conformation of progesterone receptor(s) in the uterus. J Biol Chem. 1973 Sep 25;248(18):6366–6374. [PubMed] [Google Scholar]
  21. NANDI S. Endocrine control of mammarygland development and function in the C3H/ He Crgl mouse. J Natl Cancer Inst. 1958 Dec;21(6):1039–1063. [PubMed] [Google Scholar]
  22. Philibert D., Ojasoo T., Raynaud J. Properties of the cytoplasmic progestin-binding protein in the rabbit uterus. Endocrinology. 1977 Dec;101(6):1850–1861. doi: 10.1210/endo-101-6-1850. [DOI] [PubMed] [Google Scholar]
  23. Philibert D., Raynaud J. P. Progesterone binding in the immature mouse and rat uterus. Steroids. 1973 Jul;22(1):89–98. doi: 10.1016/0039-128x(73)90073-1. [DOI] [PubMed] [Google Scholar]
  24. Philibert D., Raynaud J. P. Progesterone binding in the immature rabbit and guinea pig uterus. Endocrinology. 1974 Mar;94(3):627–632. doi: 10.1210/endo-94-3-627. [DOI] [PubMed] [Google Scholar]
  25. Puca G. A., Bresciani F. Receptor molecule for oestrogens from rat uterus. Nature. 1968 Jun 8;218(5145):967–969. doi: 10.1038/218967a0. [DOI] [PubMed] [Google Scholar]
  26. Rao B. R., Wiest W. G., Allen W. M. Progesterone "receptor" in rabbit uterus. I. Characterization and estradiol-17beta augmentation. Endocrinology. 1973 Apr;92(4):1229–1240. doi: 10.1210/endo-92-4-1229. [DOI] [PubMed] [Google Scholar]
  27. Rosen J. M., O'Neal D. L., McHugh J. E., Comstock J. P. Progesterone-mediated inhibition of casein mRNA and polysomal casein synthesis in the rat mammary gland during pregnancy. Biochemistry. 1978 Jan 24;17(2):290–297. doi: 10.1021/bi00595a016. [DOI] [PubMed] [Google Scholar]
  28. Saffran J., Loeser B. K., Bohnett S. A., Gray M. A., Faber L. E. The binding of 5 alpha-pregnane-3,20-dione by cytosol and nuclear preparations of guinea pig uterus. Endocrinology. 1978 Apr;102(4):1088–1098. doi: 10.1210/endo-102-4-1088. [DOI] [PubMed] [Google Scholar]
  29. Shyamala G. Specific cytoplasmic glucocorticoid hormone receptors in lactating mammary glands. Biochemistry. 1973 Jul 31;12(16):3085–3090. doi: 10.1021/bi00740a022. [DOI] [PubMed] [Google Scholar]
  30. Shyanala G., Nandi S. Interactions of 6,7-3H-17 -estradiol with the mouse lactating mammary tissue in vivo and in vitro. Endocrinology. 1972 Oct;91(4):861–867. doi: 10.1210/endo-91-4-861. [DOI] [PubMed] [Google Scholar]
  31. Toft D., O'Malley B. W. Target tissue receptors for progesterone: the influence of estrogen treatment. Endocrinology. 1972 Apr;90(4):1041–1045. doi: 10.1210/endo-90-4-1041. [DOI] [PubMed] [Google Scholar]
  32. Toft D., Shyamala G., Gorski J. A receptor molecule for estrogens: studies using a cell-free system. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1740–1743. doi: 10.1073/pnas.57.6.1740. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES