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. 1979 Feb 15;178(2):449–454. doi: 10.1042/bj1780449

The effect of colchicine on human blood platelets under conditions of short-term incubation.

A Ribbi-Jaffe, R Apitz-Castro
PMCID: PMC1186534  PMID: 444222

Abstract

The effects of colchicine on ADP-induced aggregation and on the phosphorylation of tubulin-like protein from human blood platelets were studied. Colchicine at 2mM concentration completely inhibits ADP-induced aggregation after 8min incubation. Under the same inhibitory conditions, phosphorylation of tubulin-like materials in intact platelets was also impaired whereas the endogenous kinase activity of tubulin, isolated through polymerization--depolymerization cycles, was not affected. It was also shown that, under conditions of maximal inhibition of both aggregation and tubulin phosphorylation, colchicine does not penetrate into the cells. The results obtained suggest that the effect of colchicine on platelet aggregation might be mainly, although not exclusively, due to a non-specific effect of the alkaloid on the plasma membrane, rather than to a direct action of the drug on the microtubular protein subunits.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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