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. 1979 May 1;179(2):327–331. doi: 10.1042/bj1790327

Localization and verification by synthesis of five antigenic sites of bovine serum albumin.

M Z Atassi, S Sakata, A L Kazim
PMCID: PMC1186630  PMID: 486084

Abstract

Recently we have shown that the major antigenic sites of bovine serum albumin exhibit functional equivalence progessively increasing with the time at which antibodies are obtained after the first immunization. Analysis of our recent immunochemical findings and the known covalent structure of bovine serum albumin have enabled us to predict the locations of five antigenic sites of bovine serum albumin. The predicted locations were synthesized, and immunochemical studies with late-course antisera showed them to constitute antigenic sites of native bovine serum albumin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atassi M. Z. Antigenic structure of myoglobin: the complete immunochemical anatomy of a protein and conclusions relating to antigenic structures of proteins. Immunochemistry. 1975 May;12(5):423–438. doi: 10.1016/0019-2791(75)90010-5. [DOI] [PubMed] [Google Scholar]
  2. Atassi M. Z., Habeeb A. F., Lee C. L. Immunochemistry of serum albumin--II. Isolation and characterization of a fragment from the first third of bovine serum albumin carrying almost all the antigenic reactivity of the protein. Immunochemistry. 1976 Jun;13(6):547–555. doi: 10.1016/0019-2791(76)90332-3. [DOI] [PubMed] [Google Scholar]
  3. Atassi M. Z. Immunochemistry of sperm-whale myoglobins prepared with various modified porphyrins and metalloporphyrins. Biochem J. 1967 Apr;103(1):29–35. doi: 10.1042/bj1030029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Atassi M. Z., Lee C. L. Boundary refinement of the lysozyme antigenic site around the disulphide bond 6-127 (site 1) by 'surface-simulation' synthesis. Biochem J. 1978 May 1;171(2):419–427. doi: 10.1042/bj1710419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Atassi M. Z., Lee C. L., Pai R. C. Enzymic and immunochemical properties of lysozyme. XVI. A novel synthetic approach to an antigenic reactive site by direct linkage of the relevant conformationally adjacent residues constituting the site. Biochim Biophys Acta. 1976 Apr 14;427(2):745–751. doi: 10.1016/0005-2795(76)90219-1. [DOI] [PubMed] [Google Scholar]
  6. Atassi M. Z., Saplin B. J. Immunochemistry of sperm whale myoglobin. I. The specific interaction of some tryptic peptides and of peptides containing all the reactive regions of the antigen. Biochemistry. 1968 Feb;7(2):688–698. doi: 10.1021/bi00842a026. [DOI] [PubMed] [Google Scholar]
  7. Atassi M. Z., Smith J. A. A proposal for the nomenclature of antigenic sites in peptides and proteins. Immunochemistry. 1978 Aug;15(8):609–610. doi: 10.1016/0161-5890(78)90016-0. [DOI] [PubMed] [Google Scholar]
  8. Atassi M. Z., Thomas A. V. Immunochemistry of sperm whale myoglobin. IV. The role of the arginine residues in the conformation and differentiation of their roles in the antigenic reactivity. Biochemistry. 1969 Aug;8(8):3385–3394. doi: 10.1021/bi00836a037. [DOI] [PubMed] [Google Scholar]
  9. Habeeb A. F., Atassi M. Z. A fragment comprising the last third of bovine serum albumin which accounts for almost all the antigenic reactivity of the native protein. J Biol Chem. 1976 Aug 10;251(15):4616–4621. [PubMed] [Google Scholar]
  10. Habeeb A. F., Atassi M. Z. Immunochemistry of serum albumin--IV. Immunochemical cross-reaction of fragments from the first third and the last third of bovine serum albumin. Immunochemistry. 1977 Jun;14(6):449–457. doi: 10.1016/0019-2791(77)90171-9. [DOI] [PubMed] [Google Scholar]
  11. Kazim A. L., Atassi M. Z. Prediction and conformation by synthesis of two antigenic sites in human haemoglobin by extrapolation from the known antigenic structure of sperm-whale myoglobin. Biochem J. 1977 Oct 1;167(1):275–278. doi: 10.1042/bj1670275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Koketsu J., Atassi M. Z. Immunochemistry of sperm-whale myoglobin. 18. Accurate delineation of the single reactive region in sequence 120-153 by study of synthetic peptides. Biochim Biophys Acta. 1973 Dec 6;328(2):289–302. [PubMed] [Google Scholar]
  13. Koketsu J., Atassi M. Z. Immunochemistry of sperm-whale myoglobin. XVI. Accurate delineation of the single region in sequence 1-55 by immunochemical studies of synthetic peptides. Some conclusions concerning antigenic structures of proteins. Immunochemistry. 1974 Jan;11(1):1–8. doi: 10.1016/0019-2791(74)90335-8. [DOI] [PubMed] [Google Scholar]
  14. LAPRESLE C. Etude de la dégradation de la sérum-albumine humaine par un extrait de rate de lapin. I. Conditions optimum d'activité et mode d'action des protéases de la rate du lapin. Bull Soc Chim Biol (Paris) 1955;37(9-10):969–975. [PubMed] [Google Scholar]
  15. Lee C. L., Atassi M. Z. Enzymic and immunochemical properties of lysozyme. Accurate definition of the antigenic site around the disulphide bridge 30-115 (site 3) by 'surface-simulation' synthesis. Biochem J. 1977 Dec 1;167(3):571–581. doi: 10.1042/bj1670571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lee C. L., Pai R. C., Atassi M. Z. Enzymic and immunochemical properties of lysozyme--XV. Delineation of the reactive site around the two central disulfides by immunochemical studies of novel synthetic peptides that contain diglycyl bridges instead of disulfides. Immunochemistry. 1976 Aug;13(8):681–687. doi: 10.1016/0019-2791(76)90209-3. [DOI] [PubMed] [Google Scholar]
  17. Matzku S., Zöller M. Iodination of immunoadsorbed antibodies: chloramine T vs lactoperoxidase. Immunochemistry. 1977 May;14(5):367–371. doi: 10.1016/0019-2791(77)90238-5. [DOI] [PubMed] [Google Scholar]

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