Abstract
Disposable pipette tips made of polymeric nylon tube with enzymes bound covalently to their inside surface and fixed to the stem of an automatic, adjustable-volume pipette holder together constitutes an immobilized enzyme pipette or 'Impette'. The present paper describes the application in research laboratories and clinics of this new development, with urease as an example in the determination of blood urea.
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Selected References
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- CHANEY A. L., MARBACH E. P. Modified reagents for determination of urea and ammonia. Clin Chem. 1962 Apr;8:130–132. [PubMed] [Google Scholar]
- Guilbault G. G., Lubrano G. J. An enzyme electrode for the amperometric determination of glucose. Anal Chim Acta. 1973 May;64(3):439–455. doi: 10.1016/S0003-2670(01)82476-4. [DOI] [PubMed] [Google Scholar]
- Meyerhoff M., Rechnitz G. A. An activated enzyme electrode for creatinine. Anal Chim Acta. 1976 Sep;85(2):277–285. doi: 10.1016/S0003-2670(01)84692-4. [DOI] [PubMed] [Google Scholar]
- Sundaram P. V., Hornby W. E. Preparation and properties of urease chemically attached to nylon tube. FEBS Lett. 1970 Oct;10(5):325–327. doi: 10.1016/0014-5793(70)80464-1. [DOI] [PubMed] [Google Scholar]
- Sundaram P. V., Igloi M. P., Wassermann R., Hinsch W. Immobilized-enzyme nylon-tube reactor for routine determination of uric acid in serum. Clin Chem. 1978 Oct;24(10):1813–1817. [PubMed] [Google Scholar]
- Sundaram P. V., Igloi M. P., Wassermann R., Hinsch W., Knoke K. J. Immobilized-enzyme nylon-tube reactors for routine determination of urea and citrulline in serum. Clin Chem. 1978 Feb;24(2):234–239. [PubMed] [Google Scholar]

