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. 1968 Aug;109(1):79–86. doi: 10.1042/bj1090079

The presence of glycopeptide material in cultured mouse mast-cell tumours

D B Thomas 1,*
PMCID: PMC1186755  PMID: 4970211

Abstract

1. Mouse mast-cell tumours P815 Y and HC were shown to contain glycoprotein material composed of glucosamine, galactosamine, sialic acid, galactose and mannose. 2. The major amino acids released after acid hydrolysis of Pronase-treated digests of the glycoprotein are aspartic acid, glutamic acid, serine, threonine, proline, glycine and alanine. The Pronase-digested material is not degraded in alkaline solution. 3. On incubation of mast cells with [35S]sulphate, heparin is the major radioactive product. However, [1-14C]glucosamine and d-[14C]glucose are incorporated largely into the glycoprotein. 4. The fate of [35S]sulphate-labelled and [1-14C]glucosamine-labelled material was studied. In each case high-molecular-weight radioactive material is released from the cells into the culture medium. The t½ of [35S]sulphate-labelled material in cells is 70hr. and that of [1-14C]-glucosamine-labelled material in cells is 40hr. 5. About 60% of the [35S]sulphate-labelled material is present in the mitochondrial and granular fraction. [1-14C]-Glucosamine-labelled material is present in both microsomal and mitochondrial and granular fractions, [14C]sialic acid being concentrated in the microsomal fraction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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