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. 1968 Aug;109(1):127–135. doi: 10.1042/bj1090127

The fractionation of nuclei from mammalian cells by zonal centrifugation

I R Johnston 1, A P Mathias 1, F Pennington 1, D Ridge 1
PMCID: PMC1186761  PMID: 4876099

Abstract

1. Purified liver nuclei from adult rats separate into two main zones when centrifuged in the slow-speed zonal rotor. One zone contains diploid nuclei, the other tetraploid. 2. The effect of age on the pattern of rat liver ploidy was examined. Tetraploid nuclei are virtually absent from young animals. They increase in proportion steadily with age. Partial hepatectomy disturbs the pattern of ploidy. 3. The zonal centrifuge permits the separation of diploid, tetraploid, octaploid and hexadecaploid nuclei from mouse liver. 4. Rat liver nuclei are isopycnic with sucrose solutions of density 1·35 at 5°.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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