Abstract
1. The binding of sodium dodecyl sulphate to proteins by equilibrium dialysis was investigated. 2. Most of the proteins studied bound 90–100% of their weight of sodium dodecyl sulphate. 3. The glycoproteins studied bound 70–100% of their weight of sodium dodecyl sulphate, calculated in terms of the polypeptide moiety of the molecule. 4. Proteins not containing S·S groups bound about 140% of their weight of sodium dodecyl sulphate. 5. Reduction of four proteins containing S·S groups caused a rise in sodium dodecyl sulphate binding to 140% of the weight of protein. 6. The apparent micellar molecular weights of the protein–sodium dodecyl sulphate complexes were measured by the dye-solubilization method; they were all found to have approximately the same micellar molecular weight (34000–41000) irrespective of the molecular weight of the protein to which they were attached.
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Selected References
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- Creeth J. M., Knight C. G. The macromolecular properties of blood-group substances. Sedimentation-velocity and viscosity measurements. Biochem J. 1967 Dec;105(3):1135–1145. doi: 10.1042/bj1051135. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EDELHOCH H., LIPPOLDT R. E. The properties of thyroglobulin. II. The effects of sodium dodecyl sulfate. J Biol Chem. 1960 May;235:1335–1340. [PubMed] [Google Scholar]
- HABEEB A. F., CASSIDY H. G., SINGER S. J. Molecular structural effects produced in proteins by reaction with succinic anhydride. Biochim Biophys Acta. 1958 Sep;29(3):587–593. doi: 10.1016/0006-3002(58)90016-7. [DOI] [PubMed] [Google Scholar]
- PUSZTAI A., MORGAN W. T. STUDIES IN IMMUNOCHEMISTRY. 22. THE AMINO ACID COMPOSITION OF THE HUMAN BLOOD-GROUP A, B, H AND LE-A SPECIFIC SUBSTANCES. Biochem J. 1963 Sep;88:546–555. doi: 10.1042/bj0880546. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reynolds J. A., Herbert S., Polet H., Steinhardt J. The binding of divers detergent anions to bovine serum albumin. Biochemistry. 1967 Mar;6(3):937–947. doi: 10.1021/bi00855a038. [DOI] [PubMed] [Google Scholar]