Absence of CCND1 gene amplification in breast tumours of BRCA1 mutation carriers

Abstract

Background/Aims—It was recently reported that significantly fewer breast tumours of BRCA1 mutation carriers overexpressed cyclin D1 and HER2 protein than tumours of age matched breast cancer cases unselected for family history. This study aimed to examine the genetic basis of this reduction by determining the frequency of tumours within this cohort showing DNA amplification of these genes.

Methods—Paraffin wax embedded sections of breast tumours from BRCA1 mutation carriers and age, grade, histological type, and tumour size matched non-familial controls that had previously been stained for cyclin D1 and HER2 protein overexpression were analysed for CCND1 and HER2 gene amplification using fluorescence in situ hybridisation.

Results—CCND1 amplification was detected in none of the 30 tumours of the BRCA1 mutation carriers and in 19 of 74 tumours of the matched controls. Of those samples previously determined to overexpress the HER2 protein, HER2 amplification was detected in one of three tumours from BRCA1 mutation carriers and in 13 of 17 tumours of the age matched non-familial cases.

Conclusion—None of the tumours of BRCA1 mutation carriers showed CCND1 amplification and only one tumour showed HER2 amplification. In contrast, a large proportion of cyclin D1 and HER2 overexpression in tumours of non-familial breast cancer cases could be accounted for by amplification of these genes. These data suggest that breast tumorigenesis in BRCA1 mutation carriers occurs by a molecular mechanism distinct from that of age matched non-familial cases.

Breast tumours of BRCA1 mutation carriers display distinct pathological features compared with unselected non-familial cases.^1–8 However, many of these features, such as higher grade, high proliferation index, and high oestrogen and progesterone receptor negativity, are similar to those seen in breast tumours of early onset non-familial cases.^{9, 10} Several studies have also reported molecular markers that distinguish hereditary from non-familial breast cancers, but few studies have taken age of onset into consideration.¹¹ We found recently that a distinct molecular immunophenotype of tumours of BRCA1 mutation carriers was influenced by age at diagnosis.^11a Significantly fewer tumours of BRCA1 mutation carriers diagnosed before the age of 50 years expressed cyclin D1 (40%) or HER2 (10%) compared with tumours of age-matched, non-familial breast cancer controls (82% and 33%, respectively).^11a No significant difference in the frequency of staining of these markers was found in tumours of carriers and controls diagnosed at age 50 years or later.

Cyclin D1 is a cell cycle regulatory protein, and protein overexpression has been reported in 42–80% of primary breast tumours^11–14 and breast cancer cell lines.¹² The CCND1 (cyclin D1) gene maps to chromosome 11q13, and this region has been reported to be amplified in approximately 30% of primary breast tumours,^{13, 15} and may be associated with poor survival.^{14, 16} Although a significant reduction in the number of tumours of BRCA1 mutation carriers that overexpress cyclin D1 compared with those of non-familial controls has been reported,^{11, 11a} the frequency of CCND1 amplification in breast tumours of BRCA1 mutation carriers has not been assessed.

HER2 protein expression has been widely reported to occur at a significantly lower frequency in breast tumours of BRCA1 mutation carriers than in tumours of non-familial cases.^{11, 11a, 17, 18} HER2 is a tyrosine kinase receptor whose overexpression in breast tumours is associated with a poor prognosis.^{19, 20} HER2 protein overexpression is associated with HER2 gene amplification in over 70% of breast tumours of non-familial cases.^21–23 However, the frequency of HER2 amplification in breast tumours of BRCA1 mutation carriers is not known.

The aim of this study was to determine the frequency of amplification of the CCND1 and HER2 genes in a series of breast tumours of BRCA1 mutation carriers and age-matched non-familial breast cancer controls, in which the frequency of protein overexpression had been determined previously.

We found that none of the tumours of BRCA1 mutation carriers showed CCND1 amplification and only one of three tumours that had previously been shown to express HER2 protein showed amplification of the HER2 gene. These results suggest that mechanisms other than gene amplification are responsible for cyclin D1 and HER2 protein overexpression in breast tumours of BRCA1 mutation carriers, and reveal a molecular distinction between tumours of BRCA1 mutation carriers and age matched non-familial breast cancer controls.

Materials and methods

PATIENTS

Patients with hereditary breast cancer were recruited through the familial cancer registry of the Cleveland Clinic Foundation. BRCA1 mutation carrier status was determined in affected probands with a cancer family history, and confirmed in tumour tissue of affected relatives.^{24, 25} Written informed consent was obtained from all subjects. The study protocol was approved by the institutional review board of the Cleveland Clinic Foundation.

Tumours from 30 BRCA1 mutation carriers were included in our study. The study population comprised 18 patients with BRCA1 185 del AG, two with BRCA1 5385 ins C, one with BRCA1 5382 ins C, one with BRCA1 3875 del 4, four with BRCA1 C1806T TER, one with BRCA1 C3508G TER, one with BRCA1 2530 del AG, and two with BRCA1 IVS 15–1 G→A mutations.

Controls were patients with breast cancer identified through the tumour registry of the Cleveland Clinic Foundation who were unselected for cancer family history. Controls were matched to each hereditary case based on age at diagnosis (± 5 years), histological type, tumour grade, and tumour size. A total of 74 matched patients with breast cancer unselected for family history were identified and included in our study. Case to control matching was as follows: 16 cases were matched to three controls; 12 cases were matched to two controls; and two cases were matched to one control each. The two BRCA1 mutation carriers who were matched to one control only had early onset medullary cancer and few candidate control matches could be identified in the tumour registry. Clinical features of cases and controls have been described previously.^11a Briefly, mean (SD) age of disease onset was 47.2 (17.9) years for the cases and 46.9 (16.7) years for the controls. The distribution frequency of tumour grade among the BRCA1 cases and controls, respectively, was as follows: 51.6% and 49.4% grade 3; 29.0% and 30.9% grade 2; and 19.3% and 19.8% grade 1. Most of the tumours (74.2% of the cases; 81.5% of the controls) were intraductal or lobular (16.1% of the cases; 13.6% of the controls). Mean (SD) tumour size was 2.49 (1.93) cm for the cases and 2.40 (1.53) cm for the controls.

FLUORESCENCE IN SITU HYBRIDISATION (FISH)

CCND1

FISH was performed on 4 μm thick paraffin wax embedded tissue sections of breast tumour tissue as described previously.²³ After dewaxing, cell conditioning using target retrieval solution (Dako, Carpinteria, California, USA), and proteinase K treatment, each section was hybridised with 10 μl of a dual labelled probe containing a spectrum green label for the centromeric region of chromosome 11 (CEP 11) and a spectrum orange label for the CCND1 locus (Vysis, Downers Grove, Illinois, USA) and coverslipped. The sections were incubated at 90°C for six minutes then overnight at 37°C in a humidified chamber. Post hybridisation washes were performed using 2× saline sodium citrate (SSC) at room temperature for five minutes, 2× SSC for three minutes at 72°C, and phosphate buffered saline (PBS)/0.1% Tween 20 (pH 7.4) for five minutes and the sections were counterstained with 15 μl of DAPI (0.1 μl/ml) in antifade solution (Ventana, Tucson, Arizona, USA). Signals were visualised using an Olympus BX40 fluorescence microscope (Olympus America, Melville, New York, USA) with three band pass filters. Two representative fields were identified in the area preselected for probe application and gene copy numbers were counted in 40 nuclei, 20 in each of the two representative fields. Tumours with a higher number of CCND1 signals compared with CEP11 signals were classified as demonstrating CCND1 amplification (fig 1A,B ▶).

Figure 1.

Fluorescence confocal microscopy photomicrographs. Infiltrating ductal carcinoma of breast demonstrating (A) normal CCND1 gene copy (red) and chromosome 11 copy (green), (B) abundant CCND1 gene copy, (C) normal HER2 gene copy (green), (D) abundant HER2 gene copy. Original magnification, ×126.

HER2

HER2 amplification was detected using an indirect digoxigenin labelled antibody protocol.^25a After dewaxing, cell conditioning using target retrieval solution (Dako) and proteinase K treatment, 10 μl of digoxigenin labelled HER2 probe (Zymed, South San Francisco, California, USA) was applied to all sections, which were then coverslipped. The sections were incubated as described above. Post hybridisation washes were performed using 2× SSC for five minutes at room temperature, 0.5× SSC for five minutes at 72°C, and PBS/0.1% Tween 20 (pH 7.4) at room temperature for five minutes. Fluorescein isothiocyanate (FITC) labelled antidigoxigenin antibody (1/50; Roche, Indianapolis, Indiana, USA) prepared in PBS (pH 7.4) containing 0.5% bovine serum albumin was applied to each slide and incubated for 30 minutes in a humidified chamber at room temperature. The sections were then washed three times in PBS/0.1% Tween 20 for five minutes each and counterstained with DAPI as described above. Two representative fields were identified in the area preselected for probe application and the gene copy number was counted for a total of 40 nuclei, 20 in each of the two representative fields. HER2 gene copy amplification was classified as present if the gene copy number was ≥5 (fig 1C,D ▶).

STATISTICAL METHODS

Fisher's exact test was used for case–control studies involving variable numbers of controls to compare the BRCA1 mutation carrier cases and non-familial breast cancer controls with respect to CCND1 and HER2 amplification frequencies.²⁶

Results

We found previously that significantly fewer breast tumours of BRCA1 mutation carriers (16 of 30) than tumours of age matched non-familial breast cancer controls (67 of 78) overexpressed cyclin D1 protein.^11a In our present study, we evaluated the molecular basis of cyclin D1 overexpression in these tumours. We found that none of 30 tumours from BRCA1 mutation carriers showed CCND1 gene amplification, whereas 19 of 74 tumours from non-familial breast cancer controls showed CCND1 gene amplification (table 1 ▶; p = 0.001). In all cases where CCND1 gene amplification occurred, tumours stained positive for cyclin D1 protein overexpression.

Table 1.

Comparison of gene amplification between patients with BRCA1 mutations and matched patients with breast cancer unselected for family history

Marker	BRCA1 mutation cases	Non-familial controls	p Values
CCND1	0/30	19/74	0.001
HER2	1/3	13/17	0.20

HER2 amplification data were captured only for samples that were previously identified as overexpressing HER2 protein.

p Values were determined using the two tailed Fisher's exact test.

We also found previously that the frequency of tumours overexpressing cyclin D1 protein in this series was influenced by age at diagnosis in both BRCA1 mutation carriers and matched non-familial breast cancer controls. A difference in the frequency of tumours staining positive for cyclin D1 between BRCA1 mutation carriers and matched non-familial breast cancer controls was observed only in tumours of patients diagnosed before the age of 50 years.^11a In our present study, a significant difference in the frequency of tumours with CCND1 amplification was seen only between BRCA1 mutation carriers and matched controls diagnosed under the age of 50 years (table 2 ▶; p = 0.002). No significant difference in the frequency of CCND1 amplification was seen in those patients diagnosed at 50 years of age or older (table 2 ▶; p = 1.0). These observations are attributed to age related differences in CCND1 amplification frequencies in tumours of non-familial breast cancer controls. We found that a significantly higher frequency of tumours of non-familial breast cancer controls diagnosed before the age of 50 years demonstrated CCND1 amplification compared with tumours of controls diagnosed at age 50 years or older (table 3 ▶; p = 0.01). These data imply that CCND1 amplification occurs infrequently in breast tumours of patients diagnosed at 50 years of age or older, irrespective of BRCA1 mutation status.

Table 2.

Comparison of gene amplification in tumours from patients with BRCA1 mutations and patients with breast cancer unselected for family history stratified by age

Marker	Age (years)	BRCA1 mutation cases	Non-familial controls	p Values
CCND1	<50	0/20	17/48	0.002
	≥50	0/10	2/26	1.0
HER2	<50	0/2	13/17	0.09
	≥50	1/1	0/0	ND

HER2 amplification data were captured only for samples that were previously identified as overexpressing HER2 protein.

p Values were determined using the two tailed Fisher's exact test.

ND, not determined.

Table 3.

Gene amplification in tumours from patients with BRCA1 mutations and patients with breast cancer unselected for family history

Age (years)	CCND1	HER2
BRCA1 mutation cases <50	0/20	0/2
≥50	0/10	1/1
p Values	ND	0.33
Non-familial controls <50	17/48	13/17
≥50	2/26	0/0
p Values	0.01	ND

HER2 amplification data were captured only for samples that were previously identified as overexpressing HER2 protein.

p Values were determined using the two tailed Fisher's exact test.

ND, not determined.

We found previously that three of 31 tumours of BRCA1 mutation carriers and 19 of 81 tumours of matched non-familial breast cancer controls overexpressed HER2 protein.^11a In our present study, we analysed all three HER2 positive BRCA1 mutation positive tumours, and 17 of the HER2 positive tumours of the controls (insufficient tumour tissue was available for two controls) for HER2 gene amplification. One of three tumours of the BRCA1 mutation carriers showed HER2 gene amplification, whereas 13 of 17 (table 1 ▶; p = 0.20) of the tumours of the matched non-familial breast cancer controls that had previously been shown to overexpress HER2 protein showed HER2 gene amplification. All of the HER2 amplification positive tumours of the controls were from patients diagnosed under the age of 50 years (table 3 ▶). The single BRCA1 mutation associated tumour that demonstrated HER2 amplification was from a patient who was diagnosed after the age of 50 years. Neither of the two tumours from the BRCA1 mutation carriers diagnosed before the age of 50 years showed HER2 gene amplification.

Discussion

We and others have reported previously that significantly fewer breast tumours of BRCA1 mutation carriers than tumours of patients with non-familial breast cancer show cyclin D1 and HER2 protein overexpression.^{11, 27} (SAJ Vaziri et al, unpublished). However, the genetic basis of this reduction is unknown. In our study, we report that this is caused, in part, by the absence of CCND1 gene amplification and a substantial reduction in the frequency of HER2 gene amplification in tumours of BRCA1 mutation carriers compared with those of matched breast cancer case controls.

We found that none of the tumours of BRCA1 mutation carriers showed CCND1 gene amplification, whereas approximately a quarter of the tumours of the non-familial breast cancer patients showed CCND1 amplification. This frequency in the control breast cancer subjects is similar to that reported elsewhere.^{13, 28} These data imply that CCND1 amplification is not a mechanism by which cyclin D1 protein overexpression occurs in tumours of BRCA1 mutation carriers. Several genes have been reported to upregulate the transcription of CCND1, including MYC,²⁹ HER2,³⁰ and the genes encoding β-catenin³¹ and the hormone estrogen.³² In our studies, β-catenin was expressed at a significantly higher frequency in tumours of BRCA1 mutation carriers than matched breast cancer controls.^11a

We also determined the frequency of HER2 amplification in those tumours that overexpressed HER2 protein in the original study. We found that HER2 amplification occurred in only three quarters of breast tumours of non-familial breast cancer cases, in agreement with published studies.^{22, 33} However, HER2 amplification occurred in only one third of breast tumours of BRCA1 mutation carriers that overexpressed HER2 protein. Unlike cyclin D1 overexpression, published data suggest that most cases of HER2 protein overexpression in tumours of non-familial breast cancer cases can be attributed to amplification of the HER2 gene.^{34, 35} Because HER2 transcriptional upregulation has been shown to occur only infrequently in non-familial breast tumours,^{25a, 35} these data suggest that the overall reduction in the frequency of HER2 overexpression may be a direct result of the reduction in the frequency of HER2 gene amplification.

Do these results imply that gene amplification is a rare mechanism in tumours of BRCA1 mutation carriers? This seems unlikely because comparative genome hybridisation approaches suggest several distinct genomic loci harbour genes that are amplified in tumours of both BRCA1 and BRCA2 mutation carriers.³⁶ In addition, a recent study reported that the MYB oncogene was frequently amplified in tumours of BRCA1 mutation carriers.³⁷ It is probable that our data reflect the presence of alternative molecular pathways of tumour development in tumours of BRCA1 mutation carriers compared with non-familial cases.

In summary, our data suggest that the reduced frequency of protein overexpression of cyclin D1 and HER2 in tumours of BRCA1 mutation carriers may be accounted for, at least in part, by the absence of amplification of the CCND1 and HER2 genes in these tumours. These data support the likelihood of distinct pathways of tumorigenesis in tumours of BRCA1 mutation carriers compared with tumours of patients with non-familial breast cancer.