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. 1968 Nov;110(1):19–25. doi: 10.1042/bj1100019

Canine submandibular-gland hyaluronidase. Purification and properties

Y H Tan 1, J M Bowness 1
PMCID: PMC1187104  PMID: 5722688

Abstract

1. Methods for the purification of dog submandibular-gland hyaluronidase from sedimentable and non-sedimentable portions of a homogenate and from the whole homogenate are presented. The method consists of three main steps: removal of mucin by acid precipitation or gel filtration on Sephadex G-200, ammonium sulphate precipitation and CM-cellulose chromatography. By this method specific activities of up to 1·28 and 0·78μmoles of N-acetylglucosamine/min./mg. of protein were obtained for the purified freeze-dried non-sedimentable hyaluronidase and for the sedimentable hyaluronidase respectively. 2. A comparison of some of the properties of the non-sedimentable and the sedimentable hyaluronidase preparation indicated that there was little difference between the two and that they both resembled lysosomal hyaluronidase from rat liver.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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