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. 1968 Dec;110(3):385–393. doi: 10.1042/bj1100385

Studies on the glycopeptides isolated from the urinary protein in heavy-chain disease

J R Clamp 1, G Dawson 1, E C Franklin 1
PMCID: PMC1187362  PMID: 5701662

Abstract

The urinary protein excreted in heavy-chain disease was separated by ion-exchange chromatography into two broad fractions designated Cra-1 and Cra-2. For a dimeric molecular weight of approx. 51000, Cra-1 contained 3–4 residues of 6-deoxy-l-galactose (l-fucose), 10 of d-mannose, 5–6 of d-galactose, 12 of 2-acetamido-2-deoxy-d-glucose (N-acetyl-d-glucosamine) and 4–5 of N-acetylneuraminic acid (sialic acid), whereas the corresponding values for Cra-2 were 2, 10, 7, 12 and 7. Cra-2 contained in addition 1 residue of 2-acetamido-2-deoxy-d-galactose (N-acetyl-d-galactosamine). Cra-1 contained an average of four oligosaccharide units, two of which contained 1 residue of 6-deoxy-l-galactose, 3 of d-mannose, 1 of d-galactose and 3 of 2-acetamido-2-deoxy-d-glucose, whereas the other two units contained the same proportions of 6-deoxy-l-galactose, d-mannose and 2-acetamido-2-deoxy-d-glucose but 2 residues of d-galactose and 2 of N-acetylneuraminic acid. Cra-2 also contained an average of four oligosaccharide units, but the range of glycopeptides was much wider, containing 0–1 residue of 6-deoxy-l-galactose, 2–3 of d-mannose, 2–3 of d-galactose, 2–3 of 2-acetamido-2-deoxy-d-glucose and 1–3 of N-acetylneuraminic acid. Possible reasons for this heterogeneity are discussed. Glycopeptides were also isolated from Cra-2 that contained 1 residue of d-mannose, 2 of d-galactose, 1 of 2-acetamido-2-deoxy-d-galactose and 0–3 of N-acetylneuraminic acid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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