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. 1968 Dec;110(3):401–406. doi: 10.1042/bj1100401

Effect of growth hormone on ribonucleic acid metabolism. The template activity of the chromatin and molecular species of ribonucleic acid synthesized after treatment with the hormone

S L Gupta 1, G P Talwar 1
PMCID: PMC1187364  PMID: 5701666

Abstract

Growth hormone stimulates the synthesis of RNA in hypophysectomized rat liver. The question whether the hormonal stimulation of RNA synthesis is due to the activation of repressed cistrons or to other factors was studied. Nuclear RNA from the livers of adult female hypophysectomized and growth-hormone-treated rats was examined for molecular homology by hybridization techniques: no new species of RNA were detected after hormone treatment. The template activity of the chromatin for RNA synthesis is also not increased by the action of growth hormone. Short- and long-pulse-labelling experiments demonstrate that the hormonal stimulation of RNA synthesis is most marked in experiments where the period of incorporation of radioactive precursors is limited to 1–2hr. It is concluded that the hormone influences essentially the rate of RNA synthesis in these tissues.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLFREY V. G., LITTAU V. C., MIRSKY A. E. On the role of of histones in regulation ribonucleic acid synthesis in the cell nucleus. Proc Natl Acad Sci U S A. 1963 Mar 15;49:414–421. doi: 10.1073/pnas.49.3.414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barker K. L., Warren J. C. Template capacity of uterine chromatin: control by estradiol. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1298–1302. doi: 10.1073/pnas.56.4.1298. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Birnboim H. C., Pène J. J., Darnell J. E. Studies on HeLa cell nuclear DNA-like RNA by RNA-DNA hybridization. Proc Natl Acad Sci U S A. 1967 Jul;58(1):320–327. doi: 10.1073/pnas.58.1.320. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Blobel G., Potter V. R. Nuclei from rat liver: isolation method that combines purity with high yield. Science. 1966 Dec 30;154(3757):1662–1665. doi: 10.1126/science.154.3757.1662. [DOI] [PubMed] [Google Scholar]
  5. CLEVER U., KARLSON P. [Induction of puff changes in the salivary gland chromosomes of Chironomus tentans by ecdysone]. Exp Cell Res. 1960 Sep;20:623–626. doi: 10.1016/0014-4827(60)90141-5. [DOI] [PubMed] [Google Scholar]
  6. Drews J., Brawerman G. Messenger RNA patterns in rat liver nuclei before and after treat-ment with growth hormone. Science. 1967 Jun 9;156(3780):1385–1386. doi: 10.1126/science.156.3780.1385. [DOI] [PubMed] [Google Scholar]
  7. Florini J. R., Breuer C. B. Amino acid incorporation into protein by cell-free systems from rat skeletal muscle. V. Effects of pituitary growth hormone on activity of ribosomes and ribonucleic acid polymerase in hypophysectomized rats. Biochemistry. 1966 Jun;5(6):1870–1876. doi: 10.1021/bi00870a013. [DOI] [PubMed] [Google Scholar]
  8. GROS F., GROS Françoise Role des acides amines dans la synthèse des acides nucléiques chez Escherichia coli. Exp Cell Res. 1958 Feb;14(1):104–131. doi: 10.1016/0014-4827(58)90218-0. [DOI] [PubMed] [Google Scholar]
  9. GROS F., HIATT H., GILBERT W., KURLAND C. G., RISEBROUGH R. W., WATSON J. D. Unstable ribonucleic acid revealed by pulse labelling of Escherichia coli. Nature. 1961 May 13;190:581–585. doi: 10.1038/190581a0. [DOI] [PubMed] [Google Scholar]
  10. HIATT H. H. A rapidly labeled RNA in rat liver nuclei. J Mol Biol. 1962 Aug;5:217–229. doi: 10.1016/s0022-2836(62)80085-0. [DOI] [PubMed] [Google Scholar]
  11. Houssais J. F., Attardi G. High molecular weight nonribosomal-type nuclear RNA and cytoplasmic messenger RNA in HeLa cells. Proc Natl Acad Sci U S A. 1966 Aug;56(2):616–623. doi: 10.1073/pnas.56.2.616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jefferson L. S., Korner A. A direct effect of growth hormone on the incorporation of precursors into proteins and nucleic acids of perfused rat liver. Biochem J. 1967 Sep;104(3):826–832. doi: 10.1042/bj1040826. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. KNOBIL E., HOTCHKISS J. GROWTH HORMONE. Annu Rev Physiol. 1964;26:47–74. doi: 10.1146/annurev.ph.26.030164.000403. [DOI] [PubMed] [Google Scholar]
  14. KORNER A. The effect of hyophysectomy and growth-hormone treatment of the rat on the incorporation of amino acids into isolated liver ribosomes. Biochem J. 1961 Nov;81:292–297. doi: 10.1042/bj0810292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Korner A. Regulation of the rate of synthesis of messenger ribonucleic acid by growth hormone. Biochem J. 1964 Sep;92(3):449–456. doi: 10.1042/bj0920449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Marushige K., Bonner J. Template properties of liver chromatin. J Mol Biol. 1966 Jan;15(1):160–174. doi: 10.1016/s0022-2836(66)80218-8. [DOI] [PubMed] [Google Scholar]
  17. Means A. R., Hamilton T. H. Early estrogen action: concomitant stimulations within two minutes of nuclear RNA synthesis and uptake of RNA precursor by the uterus. Proc Natl Acad Sci U S A. 1966 Nov;56(5):1594–1598. doi: 10.1073/pnas.56.5.1594. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. RALPH R. K., BELLAMY A. R. ISOLATION AND PURIFICATION OF UNDEGRADED RIBONUCLEIC ACIDS. Biochim Biophys Acta. 1964 May 18;87:9–16. doi: 10.1016/0926-6550(64)90041-6. [DOI] [PubMed] [Google Scholar]
  19. Scherrer K., Marcaud L., Zajdela F., London I. M., Gros F. Patterns of RNA metabolism in a differentiated cell: a rapidly labeled, unstable 60S RNA with messenger properties in duck erythroblasts. Proc Natl Acad Sci U S A. 1966 Nov;56(5):1571–1578. doi: 10.1073/pnas.56.5.1571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Shearer R. W., McCarthy B. J. Evidence for ribonucleic acid molecules restricted to the cell nucleus. Biochemistry. 1967 Jan;6(1):283–289. doi: 10.1021/bi00853a044. [DOI] [PubMed] [Google Scholar]
  21. Shih A. Y., Eisenstadt J., Lengyel P. On the relation between ribonucleic acid synthesis and peptide chain initiation in E. coli. Proc Natl Acad Sci U S A. 1966 Nov;56(5):1599–1605. doi: 10.1073/pnas.56.5.1599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. TALWAR G. P., PANDA N. C., SARIN G. S., TOLANI A. J. Effect of growth hormone on ribonucleic acid metabolism. l. Incorporation of radioactive phosphate into ribonucleic acid fractions of rat liver. Biochem J. 1962 Jan;82:173–175. doi: 10.1042/bj0820173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. TALWAR G. P., SEGAL S. J. PREVENTION OF HORMONE ACTION BY LOCAL APPLICATION OF ACTINOMYCIN D. Proc Natl Acad Sci U S A. 1963 Aug;50:226–230. doi: 10.1073/pnas.50.2.226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Tawler G. P., Gupta S. L., Gros F. Effect of growth hormone on ribonucleic acid metabolism. 3. Nature and characteristics of nuclear subfractions stimulated by hormone treatment. Biochem J. 1964 Jun;91(3):565–572. doi: 10.1042/bj0910565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. UI H., MUELLER G. C. THE ROLE OF RNA SYNTHESIS IN EARLY ESTROGEN ACTION. Proc Natl Acad Sci U S A. 1963 Aug;50:256–260. doi: 10.1073/pnas.50.2.256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Widnell C. C., Tata J. R. Additive effects of thyroid hormone, growth hormone and testosterone on deoxyribonucleic acid-dependent ribonucleic acid polymerase in rat-liver nuclei. Biochem J. 1966 Feb;98(2):621–629. doi: 10.1042/bj0980621. [DOI] [PMC free article] [PubMed] [Google Scholar]

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