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. 1968 Dec;110(3):407–412. doi: 10.1042/bj1100407

The distribution of the components of mixed-function oxidase between the rough and the smooth endoplasmic reticulum of liver cells

J L Holtzman 1, T E Gram 1, P L Gigon 1, J R Gillette 1
PMCID: PMC1187365  PMID: 5705490

Abstract

Mixed-function oxidase activity, when measured by the N-demethylation of ethylmorphine or the hydroxylation of aniline, is significantly higher in the smooth hepatic endoplasmic reticulum than in the rough. In the rabbit the smooth membrane/rough membrane activity ratios are significantly greater than 1 whether the activities are expressed per g. of liver (ratio 5), per mg. of protein (ratio 3–5), per μg. of phospholipid phosphorus (ratio 2), per unit of cytochrome P-450 (ratio 1·7) or per unit of NADPH–cytochrome c reductase activity (ratio 2). On the other hand, if the activities are normalized to the NADPH–cytochrome P-450 reductase, there is no significant difference between the rough and smooth membranes. These results suggest that, in the rabbit, the rate-limiting step is the reduction of cytochrome P-450. In contrast, in the rat the difference in activities can be explained by differences in the concentration of cytochrome P-450.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. COOPER D. Y., LEVIN S., NARASIMHULU S., ROSENTHAL O. PHOTOCHEMICAL ACTION SPECTRUM OF THE TERMINAL OXIDASE OF MIXED FUNCTION OXIDASE SYSTEMS. Science. 1965 Jan 22;147(3656):400–402. doi: 10.1126/science.147.3656.400. [DOI] [PubMed] [Google Scholar]
  3. Dallner G., Siekevitz P., Palade G. E. Phospholipids in hepatic microsomal membranes during development. Biochem Biophys Res Commun. 1965 Jul 12;20(2):142–148. doi: 10.1016/0006-291x(65)90337-2. [DOI] [PubMed] [Google Scholar]
  4. Dallner G., Siekevitz P., Palade G. E. Synthesis of microsomal membranes and their enzymic constituents in developing rat liver. Biochem Biophys Res Commun. 1965 Jul 12;20(2):135–141. doi: 10.1016/0006-291x(65)90336-0. [DOI] [PubMed] [Google Scholar]
  5. ESTABROOK R. W., COOPER D. Y., ROSENTHAL O. THE LIGHT REVERSIBLE CARBON MONOXIDE INHIBITION OF THE STEROID C21-HYDROXYLASE SYSTEM OF THE ADRENAL CORTEX. Biochem Z. 1963;338:741–755. [PubMed] [Google Scholar]
  6. FOUTS J. R. The metabolism of drugs by subfractions of hepatic microsomes. Biochem Biophys Res Commun. 1961 Dec 20;6:373–378. doi: 10.1016/0006-291x(61)90148-6. [DOI] [PubMed] [Google Scholar]
  7. Gigon P. L., Gram T. E., Gillette J. R. Effect of drug substrates on the reduction of hepatic microsomal cytochrome P-450 by NADPH. Biochem Biophys Res Commun. 1968 May 23;31(4):558–562. doi: 10.1016/0006-291x(68)90514-7. [DOI] [PubMed] [Google Scholar]
  8. Gram T. E., Rogers L. A., Fouts J. R. Further studies on the metabolism of drugs by subfractions of hepatic microsomes. J Pharmacol Exp Ther. 1967 Mar;155(3):479–493. [PubMed] [Google Scholar]
  9. Miyake Y., Langraf W., Mason H. S. An electron spin resonance study of liver and adrenal mixed function oxidases near the temperature of liquid helium. J Biol Chem. 1967 Feb 10;242(3):393–397. [PubMed] [Google Scholar]
  10. NASH T. The colorimetric estimation of formaldehyde by means of the Hantzsch reaction. Biochem J. 1953 Oct;55(3):416–421. doi: 10.1042/bj0550416. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. PHILLIPS A. H., LANGDON R. G. Hepatic triphosphopyridine nucleotide-cytochrome c reductase: isolation, characterization, and kinetic studies. J Biol Chem. 1962 Aug;237:2652–2660. [PubMed] [Google Scholar]
  12. REMMER H., MERKER H. J. EFFECT OF DRUGS ON THE FORMATION OF SMOOTH ENDOPLASMIC RETICULUM AND DRUG-METABOLIZING ENZYMES. Ann N Y Acad Sci. 1965 Mar 12;123:79–97. doi: 10.1111/j.1749-6632.1965.tb12247.x. [DOI] [PubMed] [Google Scholar]
  13. Remmer H., Schenkman J., Estabrook R. W., Sasame H., Gillette J., Narasimhulu S., Cooper D. Y., Rosenthal O. Drug interaction with hepatic microsomal cytochrome. Mol Pharmacol. 1966 Mar;2(2):187–190. [PubMed] [Google Scholar]
  14. Schenkman J. B., Frey I., Remmer H., Estabrook R. W. Sex differences in drug metabolism by rat liver microsomes. Mol Pharmacol. 1967 Nov;3(6):516–525. [PubMed] [Google Scholar]

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