Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1968 Dec;110(3):449–456. doi: 10.1042/bj1100449

Studies on flavonoid metabolism. Metabolism of (+)-catechin in the guinea pig

N P Das 1, L A Griffiths 1
PMCID: PMC1187371  PMID: 5755325

Abstract

1. Administration of (+)-catechin to the guinea pig gives rise to a number of phenolic acids and lactones, which have been identified by chromatographic and spectrophotometric methods. The major phenolic acid metabolite is m-hydroxybenzoic acid and the major lactone metabolite is δ-(3-hydroxyphenyl)-γ-valerolactone. 2. The phenolic acid and lactone metabolites are excreted in both free and conjugated forms, including their glucuronides and to a lesser degree their ethereal sulphates. 3. Administration of certain of the metabolites isolated has permitted certain sequential relationships of these intermediates to be established. 4. Degradation of (+)-catechin in the guinea pig is effected at least in part by the gut microflora and is suppressed by aureomycin plus phthaloyl-sulphathiazole.

Full text

PDF
449

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOOTH A. N., EMERSON O. H., JONES F. T., DEEDS F. Urinary metabolites of caffeic and chlorogenic acids. J Biol Chem. 1957 Nov;229(1):51–59. [PubMed] [Google Scholar]
  2. DODGSON K. S., SPENCER B. Studies on sulphatases. 4. Arylsulphatase and beta-glucuronidase concentrates from limpets. Biochem J. 1953 Sep;55(2):315–320. doi: 10.1042/bj0550315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Das N. P., Griffiths L. A. Studies on flavonoid metabolism. Biosynthesis of (+)-[14C]catechin by the plant Uncaria gambir Roxb. Biochem J. 1967 Oct;105(1):73–77. doi: 10.1042/bj1050073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Das N. P., Griffiths L. A. Studies on flavonoid metabolism. Metabolism of flavone in the guinea pig. Biochem J. 1966 Feb;98(2):488–492. doi: 10.1042/bj0980488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. ELLIOTT T. H., PARKE D. V., WILLIAMS R. T. Studies in detoxication. 79. The metabolism of cyclo [14C] hexane and its derivatives. Biochem J. 1959 Jun;72(2):193–200. doi: 10.1042/bj0720193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Griffiths L. A. Studies on flavonoid metabolism. Identification of the metabolities of (+)-catechin in rat urine. Biochem J. 1964 Jul;92(1):173–179. doi: 10.1042/bj0920173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hanson S. W., Mills G. T., Williams R. T. A study of the determination of glucuronic acid by the naphthoresorcinol reaction, with the photoelectric absorptiometer. Biochem J. 1944;38(3):274–279. doi: 10.1042/bj0380274. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KAMIL I. A., SMITH J. N., WILLIAMS R. T. Studies in detoxication. 41. A study of the optical rotations of the amides and triacetyl methyl esters of some biosynthetic substituted phenylglucuronides. Biochem J. 1951 Dec;50(2):235–240. doi: 10.1042/bj0500235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. NAKAGAWA Y., SHETLAR M. R., WENDER S. H. SPECTRAL IDENTIFICATION STUDIES OF PHENOLIC ACIDS USING ALUMINUM CHLORIDE. Anal Biochem. 1964 Mar;7:374–378. doi: 10.1016/0003-2697(64)90146-0. [DOI] [PubMed] [Google Scholar]
  10. NAKAGAWA Y., SHETLAR M. R., WENDER S. H. URINARY PRODUCTS FROM QUERCETIN IN NEOMYCIN-TREATED RATS. Biochim Biophys Acta. 1965 Feb 15;97:233–241. doi: 10.1016/0304-4165(65)90087-5. [DOI] [PubMed] [Google Scholar]
  11. Patton A. R., Foreman E. M. Glycine Reagent for Paper Chromatograms. Science. 1949 Apr 1;109(2831):339–339. doi: 10.1126/science.109.2831.339. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES