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. 1968 Dec;110(4):677–682. doi: 10.1042/bj1100677

Purification of a diketo acid hydrolase from rat liver and its use for the enzymic determination of 3,5-dioxohexanoate (triacetate)

D J H Brock 1,*, D H Williamson 1,†
PMCID: PMC1187439  PMID: 5704814

Abstract

1. A diketo acid hydrolase was purified about 100-fold from rat liver and some of its properties were studied. 2. Comparison of its activity with 3,5-dioxohexanoate (triacetate) and fumarylacetoacetate at various stages during the purification suggests that it is identical with 4-fumarylacetoacetate fumarylhydrolase. 3. The acetoacetate formed on cleavage of 3,5-dioxohexanoate by the hydrolase can be determined either with 3-hydroxybutyrate dehydrogenase or with 3-oxo acid CoA-transferase and 3-hydroxyacyl-CoA dehydrogenase. These reactions form the basis of enzymic assays for 3,5-dioxohexanoate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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