Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1969 Jan;111(1):91–95. doi: 10.1042/bj1110091

Enzymic degradation of heparin A sulphamidase and a sulphoesterase from Flavobacterium heparinum

Carl P Dietrich 1
PMCID: PMC1187498  PMID: 5775690

Abstract

A sulphamidase and a sulphoesterase were isolated from adapted cells of Flavobacterium heparinum. These enzymes were partially purified from the `heparinases' present in the bacterial extracts and characterized. The sulphamidase has a high specificity for glucosamine N-sulphate and glucosamine 2,6-disulphate. The activity decreases sharply with increasing molecular weight of the substrates tested. The sulphamidase and the sulphoesterase activities were distinguished from each other by their different sensitivities to concentration of phosphate ion and to temperature. The importance of these enzymes in the study of the structure of heparin is discussed.

Full text

PDF
91

Images in this article

92-1PLATE 1
on p.92-1
92-2PLATE 2
on p.92-2
92-3PLATE 3
on p.92-3

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dietrich C. P. Novel heparin degradation products. Isolation and characterization of novel disaccharides and oligosaccharides produced from heparin by bacterial degradation. Biochem J. 1968 Jul;108(4):647–654. doi: 10.1042/bj1080647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. KORN E. D., PAYZA A. N. The degradation of heparin by bacterial enzymes. II. Acetone powder extracts. J Biol Chem. 1956 Dec;223(2):859–864. [PubMed] [Google Scholar]
  3. KORN E. D. The degradation of heparin by bacterial enzymes. III. A comparison of the degradation of heparin, hyaluronic acid, and chondroitin sulfate. J Biol Chem. 1957 Jun;226(2):841–844. [PubMed] [Google Scholar]
  4. LINKER A., SAMPSON P. The enzymic degradation of heparitin sulfate. Biochim Biophys Acta. 1960 Sep 23;43:366–368. doi: 10.1016/0006-3002(60)90460-1. [DOI] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Linker A., Hovingh P. The enzymatic degradation of heparin and heparitin sulfate. I. The fractionation of a crude heparinase from flavobacteria. J Biol Chem. 1965 Oct;240(10):3724–3728. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES