Abstract
1. Polymers of orosomucoid were produced in two molecular shapes, filamentous (`chain') and spherical (`ball'), by heating the sodium salt of the monomer in either water or high concentrations of sodium chloride. An `intermediate' state containing both shapes in various proportions was found in preparations obtained by polymerizing orosomucoid in intermediate concentrations of sodium chloride. 2. The filamentous form of polymer was found to inhibit strongly the haemagglutination of some (`sensitive') strains but not of other (`insensitive') strains of influenza virus; the `intermediate' form feebly inhibited haemagglutination by `sensitive' strains. 3. The filamentous form agglutinated both `sensitive' and `insensitive' strains of virus; the other forms of polymer did not. It also inhibited multiplication of both `sensitive' and `insensitive' strains when inoculated into embryonated and de-embryonated eggs. 4. The `intermediate' and spherical forms of the polymer had no effect on the virus multiplication. 5. Polymers of orosomucoid from which neuraminic acid had been split off had no detectable effect on influenza viruses.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BARKER S. A., WHITEHEAD P. H. SOME IMMUNOCHEMICAL STUDIES ON OROSOMUCOID. Clin Chim Acta. 1963 Nov;8:848–854. doi: 10.1016/0009-8981(63)90006-8. [DOI] [PubMed] [Google Scholar]
- CHOPPIN P. W., STOECKENIUS W. INTERACTIONS OF ETHER-DISRUPTED INFLUENZA A2 VIRUS WITH ERYTHROCYTES, INHIBITORS, AND ANTIBODIES. Virology. 1964 Apr;22:482–492. doi: 10.1016/0042-6822(64)90069-8. [DOI] [PubMed] [Google Scholar]
- Clark W. M., Lubs H. A. The Colorimetric Determination of Hydrogen Ion Concentration and its Applications in Bacteriology : I. J Bacteriol. 1917 Jan;2(1):1–34. doi: 10.1128/jb.2.1.1-34.1917. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Easton J. A., Hardwicke J., Whitehead P. H. The estimation of two alpha(1) glycoproteins (orosomucoid and another alpha(1) acid glycoprotein) in health and disease. J Clin Pathol. 1962 Nov;15(6):585–590. doi: 10.1136/jcp.15.6.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOYLE L., HORNE R. W., WATERSON A. P. The structure and composition of the myxoviruses. II. Components released from the influenza virus particle by ether. Virology. 1961 Apr;13:448–459. doi: 10.1016/0042-6822(61)90276-8. [DOI] [PubMed] [Google Scholar]
- Hoyle L., Hana L. The chemical reactions of influenza virus proteins. J Pathol Bacteriol. 1966 Oct;92(2):447–460. doi: 10.1002/path.1700920224. [DOI] [PubMed] [Google Scholar]
- Ikenaka T., Bammerlin H., Kaufmann H., Schmid K. The amino-terminal peptide of alpha-1-acid glycoprotein. J Biol Chem. 1966 Dec 10;241(23):5560–5563. [PubMed] [Google Scholar]
- Lobodzińska M., Morawiecki A., Zielińska J., Skurska Z. The biologic action of orosomucoid and its polymer on the Asiatic influenza virus. Arch Immunol Ther Exp (Warsz) 1966;14(4):454–463. [PubMed] [Google Scholar]
- NOLL H., AOYAGI T., ORLANDO J. The structural relationship of sialidase to the influenza virus surface. Virology. 1962 Sep;18:154–157. doi: 10.1016/0042-6822(62)90193-9. [DOI] [PubMed] [Google Scholar]
- Spragg S. P., Halsall H. B., Flewett T. H., Barclay G. R. The thermal polymerization of orosomucoid. Biochem J. 1969 Feb;111(3):345–352. doi: 10.1042/bj1110345. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Whitehead P. H., Flewett T. H., Foster J. R., Sammons H. G. Inhibition of influenza virus haemagglutination by polymerized orosomucoid. Nature. 1965 Nov 27;208(5013):915–916. doi: 10.1038/208915a0. [DOI] [PubMed] [Google Scholar]