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. 1969 Mar;112(1):35–39. doi: 10.1042/bj1120035

Hormonal steroids in the tissue of induced rat mammary tumours

G Briziarelli 1, A Lusignani 1, C Pedrali 1
PMCID: PMC1187639  PMID: 5774503

Abstract

The possible presence of steroids in the tissue of induced hormone-dependent rat mammary tumours was investigated. The method used involves a preliminary extraction of tumours followed by chemical separation and thin-layer chromatography. The identified compounds were cholesterol, androst-4-ene-3,17-dione, 5β-androst-1-ene-3,17-dione, androsta-1,4-diene-3,17-dione and oestrone. This is the first report of the presence of these steroids in the tissue of an experimental tumour of a non-endocrine organ. In particular 5β-androst-1-ene-3,17-dione has not previously been identified from natural sources.

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Selected References

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  1. BONGIOVANNI A. M., EBERLEIN W. R., THOMAS P. Z. Use of an organic base in the Zimmermann reaction. J Clin Endocrinol Metab. 1957 Feb;17(2):331–332. doi: 10.1210/jcem-17-2-331. [DOI] [PubMed] [Google Scholar]
  2. BREUER H. [Comparative studies on aromatic neutral steroids in man]. Acta Endocrinol (Copenh) 1962 May;40:111–122. [PubMed] [Google Scholar]
  3. Dixon R., Jones S., Pennington G. W. The in vivo transformation of cortisol to 1 beta-hydroxytetrahydrocortisone. Steroids. 1968 May;11(5):693–697. doi: 10.1016/s0039-128x(68)80015-7. [DOI] [PubMed] [Google Scholar]
  4. GUAL C., MORATO T., HAYANO M., GUT M., DORFMAN R. I. Biosynthesis of estrogens. Endocrinology. 1962 Dec;71:920–925. doi: 10.1210/endo-71-6-920. [DOI] [PubMed] [Google Scholar]
  5. HUGGINS C., BRIZIARELLI G., SUTTON H., Jr Rapid induction of mammary carcinoma in the rat and the influence of hormones on the tumors. J Exp Med. 1959 Jan 1;109(1):25–42. doi: 10.1084/jem.109.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hobbs C. B., Miller A. L. Review of endocrine syndromes associated with tumours of non-endocrine origin. J Clin Pathol. 1966 Mar;19(2):119–127. doi: 10.1136/jcp.19.2.119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. KING R. J., GORDON J., HELFENSTEIN J. E. THE METABOLISM OF TESTOSTERONE BY TISSUE FROM NORMAL AND NEOPLASTIC RAT BREAST. J Endocrinol. 1964 May;29:103–110. doi: 10.1677/joe.0.0290103. [DOI] [PubMed] [Google Scholar]
  8. King J. B., Panattoni M., Gordon J., Baker R. The metabolism of steroids by tissue from normal and neoplastic rat breast. J Endocrinol. 1965 Aug;33(1):127–132. doi: 10.1677/joe.0.0330127. [DOI] [PubMed] [Google Scholar]
  9. MILLER W. R., TURNER C. W., FUKUSHIMA D. K., SALAMON I. I. The identification of C19 steroids in bovine feces. J Biol Chem. 1956 May;220(1):221–225. [PubMed] [Google Scholar]
  10. MORATO T., HAYANO M., DORFMAN R. I., AXELROD L. R. The intermediate steps in the biosynthesis of estrogens from androgens. Biochem Biophys Res Commun. 1961 Dec 20;6:334–338. doi: 10.1016/0006-291x(61)90140-1. [DOI] [PubMed] [Google Scholar]
  11. Schneider J. J., Bhacca N. S. 1-beta-Hydroxylation of 3-alpha,20-beta,21-tetrahydroxy 5-beta-pregnan-11-one and of other 5-beta-steroids in a man and by surviving liver slices of the guinea pig. J Biol Chem. 1966 Nov 25;241(22):5313–5324. [PubMed] [Google Scholar]
  12. VISCHER E., WETTSTEIN A. Mikrobiologische Reaktionen; Seitenketten-Abbau und Dehydrierung bei Steroiden. Experientia. 1953 Oct 15;9(10):371–372. doi: 10.1007/BF02167636. [DOI] [PubMed] [Google Scholar]

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