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. 1969 Mar;112(1):51–59. doi: 10.1042/bj1120051

Intestinal disaccharidase activities in the chick

R C Siddons 1
PMCID: PMC1187641  PMID: 5774506

Abstract

1. Disaccharidase activities of the small and large intestines of the chick were studied. 2. Homogenates of the small intestine readily hydrolysed maltose, sucrose and palatinose (6-O-α-d-glucopyranosyl-d-fructose), hydrolysed lactose slowly and did not hydrolyse trehalose and cellobiose. 3. Within the small intestine the disaccharidases were located mainly in the intestinal wall; the activity in the contents accounted for less than 5% of the total activity. 4. The disaccharidases were non-uniformly distributed along the small intestine, the activities being greatest in the middle section. 5. The disaccharidase activities increased with age between 1 and 43 days. 6. Homogenates of the large intestine and contents readily hydrolysed maltose, sucrose, palatinose and lactose and hydrolysed cellobiose and trehalose slowly. 7. The large-intestinal disaccharidases were located mainly in the contents. 8. Similar Km and pH optimum values were found for the maltase, sucrase and palatinase activities of the large and small intestines. 9. The lactase activity of the large intestine was markedly affected by diet and had different Km and pH values from the small intestinal lactase. 10. Low activities of intestinal disaccharidase were found in 12-day-old embryos and marked increases in the intestinal disaccharidases of the developing embryo occurred 2–3 days before hatching.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALVAREZ A., SAS J. beta-Galactosidase changes in the developing intestinal tract of the rat. Nature. 1961 May 27;190:826–827. doi: 10.1038/190826b0. [DOI] [PubMed] [Google Scholar]
  2. ANDERSON C. M., MESSER M., TOWNLEY R. R., FREEMAN M. Intestinal sucrase and isomaltase deficiency in two siblings. Pediatrics. 1963 Jun;31:1003–1010. [PubMed] [Google Scholar]
  3. AURICCHIO S., RUBINO A., TOSI R., SEMENZA G., LANDOLT M., KISTLER H., PRADER A. DISACCHARIDASE ACTIVITIES IN HUMAN INTESTINAL MUCOSA. Enzymol Biol Clin (Basel) 1963;74:193–208. doi: 10.1159/000458059. [DOI] [PubMed] [Google Scholar]
  4. Akrabawi S. S., Kratzer F. H. Effects of arginine or serine on the requirement for glycine by the chick. J Nutr. 1968 May;95(1):41–48. doi: 10.1093/jn/95.1.41. [DOI] [PubMed] [Google Scholar]
  5. Asp N. G., Dahlqvist A. Rat small-intestinal beta-galactosidases. Separation by ion-exchange chromatography and gel filtration. Biochem J. 1968 Feb;106(4):841–845. doi: 10.1042/bj1060841. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. BLAIR D. G., TUBA J. Rat intestinal sucrase. I. Intestinal distribution and reaction kinetics. Can J Biochem Physiol. 1963 Apr;41:905–916. [PubMed] [Google Scholar]
  7. BLAIR D. G., YAKIMETS W., TUBA J. Rat intestinal sucrase. II. The effects of rat age and sex and of diet on sucrase activity. Can J Biochem Physiol. 1963 Apr;41:917–929. [PubMed] [Google Scholar]
  8. COHN M. Contributions of studies on the beta-galactosidase of Escherichia coli to our understanding of enzyme synthesis. Bacteriol Rev. 1957 Sep;21(3):140–168. doi: 10.1128/br.21.3.140-168.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. DAHLQVIST A. METHOD FOR ASSAY OF INTESTINAL DISACCHARIDASES. Anal Biochem. 1964 Jan;7:18–25. doi: 10.1016/0003-2697(64)90115-0. [DOI] [PubMed] [Google Scholar]
  10. DAHLQVIST A. The location of carbohydrases in the digestive tract of the pig. Biochem J. 1961 Feb;78:282–288. doi: 10.1042/bj0780282. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. DIXON M. The determination of enzyme inhibitor constants. Biochem J. 1953 Aug;55(1):170–171. doi: 10.1042/bj0550170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. DOELL R. G., KRETCHMER N. Studies of small intestine during development. I. Distribution and activity of beta-galactosidase. Biochim Biophys Acta. 1962 Aug 13;62:353–362. doi: 10.1016/0006-3002(62)90097-5. [DOI] [PubMed] [Google Scholar]
  13. HERZENBERG L. A., HERZENBERG L. A. Adaptation to lactose. Nutr Rev. 1959 Mar;17(3):65–67. doi: 10.1111/j.1753-4887.1959.tb06395.x. [DOI] [PubMed] [Google Scholar]
  14. Jos J., Frezal J., Rey J., Lamy M. Histochemical localization of intestinal disaccharidases: application to peroral biopsy specimens. Nature. 1967 Feb 4;213(5075):516–518. doi: 10.1038/213516a0. [DOI] [PubMed] [Google Scholar]
  15. KOLDOVSKY O., CHYTIL F. POSTNATAL DEVELOPMENT OF BETA-GALACTOSIDASE ACTIVITY IN THE SMALL INTESTINE OF THE RAT. EFFECT OF ADRENALECTOMY AND DIET. Biochem J. 1965 Jan;94:266–270. doi: 10.1042/bj0940266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Koldovský O., Noack R., Schenk G., Jirsová V., Heringová A., Braná H. Activity of beta-galactosidase in homogenates and isolated microvilli fraction of jejunal mucosa from suckling rats. Biochem J. 1965 Aug;96(2):492–494. doi: 10.1042/bj0960492. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. LAWS B. M., MOORE J. H. SOME OBSERVATIONS ON THE PANCREATIC AMYLASE AND INTESTINAL MALTASE OF THE CHICK. Can J Biochem Physiol. 1963 Oct;41:2107–2121. [PubMed] [Google Scholar]
  18. LEDERBERG J. The beta-d-galactosidase of Escherichia coli, strain K-12. J Bacteriol. 1950 Oct;60(4):381–392. doi: 10.1128/jb.60.4.381-392.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  20. PRADER A., AURICCHIO S. DEFECTS OF INTESTINAL DISACCHARIDE ABSORPTION. Annu Rev Med. 1965;16:345–358. doi: 10.1146/annurev.me.16.020165.002021. [DOI] [PubMed] [Google Scholar]
  21. Pilson M. E., Kelly A. L. Composition of the Milk from Zalophus californianus, the California Sea Lion. Science. 1962 Jan 12;135(3498):104–105. doi: 10.1126/science.135.3498.104. [DOI] [PubMed] [Google Scholar]
  22. Plimmer R. H., Rosedale J. L. Distribution of Enzymes in the Alimentary Canal of the Chicken. Biochem J. 1922;16(1):23–26. doi: 10.1042/bj0160023. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. RUBINO A., ZIMBALATTI F., AURICCHIO S. INTESTINAL DISACCHARIDASE ACTIVITIES IN ADULT AND SUCKLING RATS. Biochim Biophys Acta. 1964 Nov 22;92:305–311. doi: 10.1016/0926-6569(64)90187-7. [DOI] [PubMed] [Google Scholar]
  24. Siddons R. C. Carbohydrase activities in the bovine digestive tract. Biochem J. 1968 Aug;108(5):839–844. doi: 10.1042/bj1080839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. WALLENFELS K., FISCHER J. [Research on lactose-splitting enzymes. X. Lactase of calf intestines]. Hoppe Seylers Z Physiol Chem. 1960 Dec 2;321:223–245. doi: 10.1515/bchm2.1960.321.1.223. [DOI] [PubMed] [Google Scholar]
  26. Welsh J. D., Walker A. Intestinal disaccharidase and alkaline phosphatase activity in the dog. Proc Soc Exp Biol Med. 1965 Nov;120(2):525–527. doi: 10.3181/00379727-120-30580. [DOI] [PubMed] [Google Scholar]

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