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. 1969 Apr;112(2):187–194. doi: 10.1042/bj1120187

Studies on the reduction of a human 19s immunoglobulin M

D Beale 1, A Feinstein 1
PMCID: PMC1187691  PMID: 4979347

Abstract

1. Reduction of a 19s immunoglobulin M with 3mm-mercaptoethanol or 0·05–0·5mm-dithiothreitol followed by alkylation gave sedimentation patterns indicating products compatible with structures consisting of one, two, three, four and five 7s sub-units. This supports the concept of a five-sub-unit structure for immunoglobulin M. 2. Reduction with 0·125mm-dithiothreitol or 20mm-cysteine produced 7s sub-units that could not be dissociated into chains in m-propionic acid. 3. By labelling (with iodo[2-14C]acetic acid) the thiol groups liberated during reduction with 0·125mm-dithiothreitol, it was possible to identify the tryptic peptides involved in the disulphide bridges that link the 7s sub-units together (inter-sub-unit bridges). 4. By further reducing and labelling (with iodo[2-14C]acetic acid) the 7s sub-units produced by 0·125mm-dithiothreitol, it was possible to identify tryptic peptides derived from intra-sub-unit bridges. 5. Sub-units produced by reduction with 20mm-cysteine proved to be unsuitable for distinguishing between inter-sub-unit bridges and intra-sub-unit bridges. 6. The possible arrangement of the interchain disulphide bridges was deduced.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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