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. 1969 May;112(4):521–525. doi: 10.1042/bj1120521

Composition of Pseudomonas aeruginosa slime

M R W Brown 1, J H Scott Foster 1, J R Clamp 1
PMCID: PMC1187742  PMID: 4240755

Abstract

1. The slime produced by eight strains of Pseudomonas aeruginosa on a number of different media was demonstrated to be qualitatively the same. Small quantitative differences may be occasioned by differences in the extraction procedure, the growth medium or the strain of organism used. 2. The slime was shown to be predominantly polysaccharide with some nucleic acid material and a small amount of protein. 3. The hydrolysed polysaccharide fraction consists mainly of glucose with smaller amounts of mannose. This accounts for some 50–60% of the total slime. In addition, there is some 5% of hyaluronic acid. The nucleic acid material represents approx. 20% of the total weight, and is composed of both RNA and DNA. 4. Minor components are protein, rhamnose and glucosamine, the protein being less than 5% of the total. 5. Hyaluronic acid is produced in greater quantities from nutrient broth than from chemically defined media, and is more firmly attached to the cells than the other components.

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Selected References

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  1. BITTER T., MUIR H. M. A modified uronic acid carbazole reaction. Anal Biochem. 1962 Oct;4:330–334. doi: 10.1016/0003-2697(62)90095-7. [DOI] [PubMed] [Google Scholar]
  2. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CARSON K. J., EAGON R. G. CELLULAR DISINTEGRATION WITH CONCOMITANT RELEASE OF SLIME AND PRODUCTION OF EXTRACELLULAR MANNAN BY PSEUDOMONAS AERUGINOSA: TWO SEPARATE PHENOMENA. Can J Microbiol. 1964 Jun;10:467–472. doi: 10.1139/m64-056. [DOI] [PubMed] [Google Scholar]
  4. CLAMP J. R., PUTNAM F. W. THE CARBOHYDRATE PROSTHETIC GROUP OF HUMAN GAMMA-GLOBULIN. J Biol Chem. 1964 Oct;239:3233–3240. [PubMed] [Google Scholar]
  5. CLAUSEN J., ANDERSEN V. ACID MUCOPOLYSACCHARIDES IN HUMAN LEUCOCYTES. Clin Chim Acta. 1963 Jul;8:505–512. doi: 10.1016/0009-8981(63)90096-2. [DOI] [PubMed] [Google Scholar]
  6. CLAUSEN J., HANSEN A. Acid mucopolysaccharides of human brain: identification by means of infra-red analysis. J Neurochem. 1963 Mar;10:165–168. doi: 10.1111/j.1471-4159.1963.tb09478.x. [DOI] [PubMed] [Google Scholar]
  7. Carlson D. M., Matthews L. W. Polyuronic acids produced by Pseudomonas aeruginosa. Biochemistry. 1966 Sep;5(9):2817–2822. doi: 10.1021/bi00873a006. [DOI] [PubMed] [Google Scholar]
  8. Clamp J. R., Dawson G., Hough L. The simultaneous estimation of 6-deoxy-L-galactose (L-fucose), D-mannose, D-galactose, 2-acetamido-2-deoxy-D-glucose (N-acetyl-D-glucosamine) and N-acetylneuraminic acid (sialic acid) in glycopeptides and glycoproteins. Biochim Biophys Acta. 1967 Nov 28;148(2):342–349. doi: 10.1016/0304-4165(67)90129-8. [DOI] [PubMed] [Google Scholar]
  9. Clarke K., Gray G. W., Reaveley D. A. The cell walls of Pseudomonas aeruginosa. General composition. Biochem J. 1967 Nov;105(2):749–754. doi: 10.1042/bj1050749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Di Ferrante N. M. The measurement of urinary mucopolysaccharides. Anal Biochem. 1967 Oct;21(1):98–106. doi: 10.1016/0003-2697(67)90087-5. [DOI] [PubMed] [Google Scholar]
  11. Edwards J. R., Hayashi J. A. Structure of a rhamnolipid from Pseudomonas aeruginosa. Arch Biochem Biophys. 1965 Aug;111(2):415–421. doi: 10.1016/0003-9861(65)90204-3. [DOI] [PubMed] [Google Scholar]
  12. Gatt R., Berman E. R. A rapid procedure for the estimation of amino sugars on a micro scale. Anal Biochem. 1966 Apr;15(1):167–171. doi: 10.1016/0003-2697(66)90262-4. [DOI] [PubMed] [Google Scholar]
  13. HAYNES W. C. Pseudomonas aeruginosa--its characterization and identification. J Gen Microbiol. 1951 Nov;5(5 Suppl):939–950. doi: 10.1099/00221287-5-5-939. [DOI] [PubMed] [Google Scholar]
  14. KERBY G. P. The excretion of glucuronic acid and of acid mucopolysaccharides in normal human urine. J Clin Invest. 1954 Aug;33(8):1168–1174. doi: 10.1172/JCI102989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Klyhn K. M., Gorrill R. H. Studies on the virulence of hospital strains of Pseudomonas aeruginosa. J Gen Microbiol. 1967 May;47(2):227–235. doi: 10.1099/00221287-47-2-227. [DOI] [PubMed] [Google Scholar]
  16. LEITNER J. G., KERBY G. P. Staining of acid mucopolysaccharides after chromatography on filter paper. Stain Technol. 1954 Sep;29(5):257–259. doi: 10.3109/10520295409115479. [DOI] [PubMed] [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  18. ORR S. F. Infra-red spectroscopic studies of some polysaccharides. Biochim Biophys Acta. 1954 Jun;14(2):173–181. doi: 10.1016/0006-3002(54)90156-0. [DOI] [PubMed] [Google Scholar]
  19. WARREN G. H., GRAY J. Studies on the properties of a polysaccharide constituent produced by Pseudomonas aeruginosa. J Bacteriol. 1955 Aug;70(2):152–157. doi: 10.1128/jb.70.2.152-157.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. WARREN G. H., GRAY J. The depolymerization of bacterial polysaccharides by hyalyronidase preparations. J Bacteriol. 1954 Feb;67(2):167–170. doi: 10.1128/jb.67.2.167-170.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. WILKINSON J. F. The extracellualr polysaccharides of bacteria. Bacteriol Rev. 1958 Mar;22(1):46–73. doi: 10.1128/br.22.1.46-73.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]

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