Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1969 May;112(5):571–578. doi: 10.1042/bj1120571

The reduction of cystine during its transport into rat jejunal everted segments and sacs

J C Crawhall 1,*, M G Davis 1
PMCID: PMC1187758  PMID: 5821723

Abstract

1. Everted segments and sacs of rat jejunum were incubated in buffer containing [35S]cystine. 2. Concentration gradients were achieved by both segments and sacs, and the effects of duration of incubation and of cystine concentration on the isotope distribution ratios were determined. 3. Kinetic constants were determined for the uptake of cystine by both segments and sacs, and the differences between the two systems are discussed. 4. Reduction to cysteine was virtually complete intracellularly and in the sac lumen. Extensive reduction in the medium occurred only when segments were incubated. 5. Anaerobiosis prevented a concentration gradient being obtained between the medium and the tissue, but had little effect on the extent of reduction to cysteine in the tissue and sac lumen. 6. It is concluded that cystine is transported by an active process into rat jejunum, where it is present almost entirely in the reduced form, and that efflux of cysteine occurs through the serosal surface.

Full text

PDF
571

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AKEDO H., CHRISTENSEN H. N. Nature of insulin action on amino acid uptake by the isolated diaphragm. J Biol Chem. 1962 Jan;237:118–122. [PubMed] [Google Scholar]
  2. Crawhall J. C., Segal S. The intracellular ratio of cysteine and cystine in various tissues. Biochem J. 1967 Nov;105(2):891–896. doi: 10.1042/bj1050891. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. MARSH H. B., ROSENBERG L. E., SEGAL S. Observations on the effect of bovine growth hormone on amino acid accumulation by rat kidney-cortex slices and intestinal segments. Endocrinology. 1962 Sep;71:516–519. doi: 10.1210/endo-71-3-516. [DOI] [PubMed] [Google Scholar]
  4. McLeod M. E., Tyor M. P. Transport of basic amino acids by hamster intestine. Am J Physiol. 1967 Jul;213(1):163–168. doi: 10.1152/ajplegacy.1967.213.1.163. [DOI] [PubMed] [Google Scholar]
  5. NEIL M. W. The absorption of cystine and cysteine from rat small intestine. Biochem J. 1959 Jan;71(1):118–124. doi: 10.1042/bj0710118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. ROBSON E. B., ROSE G. A. The effect of intravenous lysine on the renal clearances of cystine, arginine and ornithine in normal subjects, in patients with cystinuria and Fanconi syndrome and in their relatives. Clin Sci. 1957 Feb;16(1):75–93. [PubMed] [Google Scholar]
  7. ROSENBERG L. E., BLAIR A., SEGAL S. Transport of amino acids by slices of rat-kidney cortex. Biochim Biophys Acta. 1961 Dec 23;54:479–488. doi: 10.1016/0006-3002(61)90088-9. [DOI] [PubMed] [Google Scholar]
  8. ROSENBERG L. E., DOWNING S. J., SEGAL S. Competitive inhibition of dibasic amino acid transport in rat kidney. J Biol Chem. 1962 Jul;237:2265–2270. [PubMed] [Google Scholar]
  9. Rosenberg L. E., Crawhall J. C., Segal S. Intestinal transport of cystine and cysteine in man: evidence for separate mechanisms. J Clin Invest. 1967 Jan;46(1):30–34. doi: 10.1172/JCI105508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Segal S., Crawhall J. C. Characteristics of cystine and cysteine transport in rat kidney cortex slices. Proc Natl Acad Sci U S A. 1968 Jan;59(1):231–237. doi: 10.1073/pnas.59.1.231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Segal S., Lowenstein L. M., Wallace A. Comparison of the transport characteristics of L-lysine by rat intestine and kidney cortex. Gastroenterology. 1968 Sep;55(3):386–391. [PubMed] [Google Scholar]
  12. WILSON T. H., WISEMAN G. The use of sacs of everted small intestine for the study of the transference of substances from the mucosal to the serosal surface. J Physiol. 1954 Jan;123(1):116–125. doi: 10.1113/jphysiol.1954.sp005036. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES