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. 1989 Oct;417:555–565. doi: 10.1113/jphysiol.1989.sp017818

Cross-bridge movement in rat cardiac muscle as a function of calcium concentration.

I Matsubara 1, D W Maughan 1, Y Saeki 1, N Yagi 1
PMCID: PMC1189283  PMID: 2621610

Abstract

1. By applying the X-ray diffraction method to chemically skinned papillary muscles of the rat, the transfer of myosin heads from the thick to the thin filaments was studied as a function of Ca2+ concentration. 2. No significant transfer of the heads occurred when the Ca2+ concentration was below the threshold of contraction (pCa 6.2). 3. During the maximum isometric contraction at pCa 4.4, 80% of the myosin heads were transferred to the thin filament. 4. When the muscle was activated isometrically at low Ca2+ concentrations (pCa 6.2-5.8), where the average tension was less than 20% of the maximum, a disproportionately large number of myosin heads were transferred to the thin filament. 5. It was concluded that a significant fraction of the heads transferred at the low Ca2+ concentrations does not produce tension.

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Selected References

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