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. 1997 Jan;61(1):62–65.

Serodiagnosis of swine pleuropneumonia due to Actinobacillus pleuropneumoniae serotypes 7 and 4 using long-chain lipopolysaccharides.

M Gottschalk 1, E Altman 1, S Lacouture 1, F De Lasalle 1, J D Dubreuil 1
PMCID: PMC1189372  PMID: 9008804

Abstract

A saline boiled extract (SBE), capsular polysaccharides (CPS) and long-chain lipopolysaccharides (LC-LPS) of Actinobacillus pleuropneumoniae serotype 7 have been evaluated in ELISA for the serodiagnosis of swine pleuropneumonia caused by this serotype. Mean optical densities (ODs) obtained with the 3 antigens using sera from negative herds as well as from animals experimentally and naturally exposed to A. pleuropneumoniae serotypes 7 or 4 were not statistically different. The positive ELISA reaction with anti-serotype 4 sera was unexpected with the CPS, which are supposed to be serotype-specific; LPS traces present in the CPS appeared to be responsible for this reaction. In addition, sera from animals exposed to A. pleuropneumoniae serotypes 5 or 10 presented cross-reactions with the SBE and the CPS, but not with the LC-LPS. Cross-reactions were mainly due to rough LPS, as shown by immunoblotting. The LC-LPS is easily obtainable and can be used for the detection of antibodies in animals infected with A. pleuropneumoniae serotypes 7 and 4.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beynon L. M., Perry M. B., Richards J. C. Structure of the capsular polysaccharide from Actinobacillus pleuropneumoniae serotype 7. Carbohydr Res. 1991 Jan 15;209:211–223. doi: 10.1016/0008-6215(91)80158-j. [DOI] [PubMed] [Google Scholar]
  2. Bossé J. T., Friendship R., Rosendal S., Fenwick B. W. Development and evaluation of a mixed-antigen ELISA for serodiagnosis of Actinobacillus pleuropneumoniae serotypes 1, 5, and 7 infections in commercial swine herds. J Vet Diagn Invest. 1993 Jul;5(3):359–362. doi: 10.1177/104063879300500309. [DOI] [PubMed] [Google Scholar]
  3. Bossé J. T., Johnson R. P., Rosendal S. Capsular polysaccharide antigens for detection of serotype-specific antibodies to Actinobacillus pleuropneumoniae. Can J Vet Res. 1990 Jun;54(3):320–325. [PMC free article] [PubMed] [Google Scholar]
  4. Bossé J. T., Johnson R. P., Rosendal S. Serodiagnosis of pleuropneumonia using enzyme-linked immunosorbent assay with capsular polysaccharide antigens of Actinobacillus pleuropneumoniae serotypes 1, 2, 5 and 7. Can J Vet Res. 1990 Oct;54(4):427–431. [PMC free article] [PubMed] [Google Scholar]
  5. Godfrey K. Statistics in practice. Comparing the means of several groups. N Engl J Med. 1985 Dec 5;313(23):1450–1456. doi: 10.1056/NEJM198512053132305. [DOI] [PubMed] [Google Scholar]
  6. Gottschalk M., Altman E., Charland N., De Lasalle F., Dubreuil J. D. Evaluation of a saline boiled extract, capsular polysaccharides and long-chain lipopolysaccharides of Actinobacillus pleuropneumoniae serotype 1 as antigens for the serodiagnosis of swine pleuropneumonia. Vet Microbiol. 1994 Nov;42(2-3):91–104. doi: 10.1016/0378-1135(94)90009-4. [DOI] [PubMed] [Google Scholar]
  7. Gottschalk M., De Lasalle F., Radacovici S., Dubreuil J. D. Evaluation of long chain lipopolysaccharides (LC-LPS) of Actinobacillus pleuropneumoniae serotype 5 for the serodiagnosis of swine pleuropneumonia. Vet Microbiol. 1994 Feb;38(4):315–327. doi: 10.1016/0378-1135(94)90150-3. [DOI] [PubMed] [Google Scholar]
  8. Inzana T. J., Mathison B. Serotype specificity and immunogenicity of the capsular polymer of Haemophilus pleuropneumoniae serotype 5. Infect Immun. 1987 Jul;55(7):1580–1587. doi: 10.1128/iai.55.7.1580-1587.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Mittal K. R., Higgins R., Larivière S., Nadeau M. Serological characterization of Actinobacillus pleuropneumoniae strains isolated from pigs in Quebec. Vet Microbiol. 1992 Sep;32(2):135–148. doi: 10.1016/0378-1135(92)90101-x. [DOI] [PubMed] [Google Scholar]
  11. Nielsen R., Plambeck T., Foged N. T. Blocking enzyme-linked immunosorbent assay for detection of antibodies to Actinobacillus pleuropneumoniae serotype 2. J Clin Microbiol. 1991 Apr;29(4):794–797. doi: 10.1128/jcm.29.4.794-797.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Radacovici S., Lallier R., Larivière S., Dubreuil J. D. Biochemical characterization of an antigenic saline extract of Actinobacillus pleuropneumoniae serotype 5 and identification of a serotype-specific antigen for ELISA serodiagnosis. Vet Microbiol. 1992 Mar;30(4):369–385. doi: 10.1016/0378-1135(92)90023-m. [DOI] [PubMed] [Google Scholar]
  13. Sidibé M., Messier S., Larivière S., Gottschalk M., Mittal K. R. Detection of Actinobacillus pleuropneumoniae in the porcine upper respiratory tract as a complement to serological tests. Can J Vet Res. 1993 Jul;57(3):204–208. [PMC free article] [PubMed] [Google Scholar]
  14. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Trottier Y. L., Wright P. F., Larivière S. Optimization and standardization of an enzyme-linked immunosorbent assay protocol for serodiagnosis of Actinobacillus pleuropneumoniae serotype 5. J Clin Microbiol. 1992 Jan;30(1):46–53. doi: 10.1128/jcm.30.1.46-53.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Willson P. J., Schipper C., Morgan E. D. The Use of an Enzyme-linked Immunosorbent Assay for Diagnosis of Actinobacillus pleuropneumoniae Infection in Pigs. Can Vet J. 1988 Jul;29(7):583–585. [PMC free article] [PubMed] [Google Scholar]

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