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. 1997 Oct;61(4):275–279.

Immunization of pigs against Streptococcus suis serotype 2 infection using a live avirulent strain.

P Busque 1, R Higgins 1, F Caya 1, S Quessy 1
PMCID: PMC1189421  PMID: 9342451

Abstract

Streptococcus suis capsular type 2 is still an important cause of economic losses in the swine industry. At the present time, vaccination of pigs against this infection is generally carried out with autogenous bacterins and results are equivocal. In this study, the protective effect of a live avirulent S. suis type 2 strain (#1330) which had induced a good protection in mice, was evaluated in swine. The experiment was performed in triplicate using 4 week-old piglets. A total of 15 piglets were vaccinated 3 times, 15 others were vaccinated 2 times, and 15 piglets were injected 3 times with sterile Todd-Hewitt broth. Using an indirect ELISA, an increase in the IgG response to S. suis antigens was noted in 27 of the 30 vaccinated piglets. On day 21 post-vaccination, all animals were challenged intravenously with a virulent S. suis type 2 strain (#999). In the 2 vaccinated groups, 26 animals were fully protected. Only 1 out of the 15 piglets vaccinated 3 times developed mild clinical signs. In the group vaccinated twice, 3 piglets showed clinical signs and 1 of them died after the challenge. In the control group, 7 animals died out of the 11 with clinical signs of infection. In conclusion, a protective immunity was observed in swine when using strain 1330. However, more studies are needed to assess the use of a live S. suis strain in a vaccine for pigs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arends J. P., Zanen H. C. Meningitis caused by Streptococcus suis in humans. Rev Infect Dis. 1988 Jan-Feb;10(1):131–137. doi: 10.1093/clinids/10.1.131. [DOI] [PubMed] [Google Scholar]
  2. Beaudoin M., Higgins R., Harel J., Gottschalk M. Studies on a murine model for evaluation of virulence of Streptococcus suis capsular type 2 isolates. FEMS Microbiol Lett. 1992 Dec 1;78(2-3):111–116. doi: 10.1016/0378-1097(92)90011-c. [DOI] [PubMed] [Google Scholar]
  3. Blouin C., Higgins R., Gottschalk M., Simard J. Evaluation of the antibody response in pigs vaccinated against Streptococcus suis capsular type 2 using a double-antibody sandwich enzyme-linked immunosorbent assay. Can J Vet Res. 1994 Jan;58(1):49–54. [PMC free article] [PubMed] [Google Scholar]
  4. Elliott S. D., Clifton-Hadley F., Tai J. Streptococcal infection in young pigs. V. An immunogenic polysaccharide from Streptococcus suis type 2 with particular reference to vaccination against streptococcal meningitis in pigs. J Hyg (Lond) 1980 Oct;85(2):275–285. doi: 10.1017/s0022172400063312. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gottschalk M., Higgins R., Jacques M., Dubreuil D. Production and characterization of two Streptococcus suis capsular type 2 mutants. Vet Microbiol. 1992 Jan;30(1):59–71. doi: 10.1016/0378-1135(92)90094-a. [DOI] [PubMed] [Google Scholar]
  6. Higgins R., Gottschalk M. An update on Streptococcus suis identification. J Vet Diagn Invest. 1990 Jul;2(3):249–252. doi: 10.1177/104063879000200324. [DOI] [PubMed] [Google Scholar]
  7. Holt M. E., Enright M. R., Alexander T. J. Immunisation of pigs with killed cultures of Streptococcus suis type 2. Res Vet Sci. 1990 Jan;48(1):23–27. [PubMed] [Google Scholar]
  8. Holt M. E., Enright M. R., Alexander T. J. Immunisation of pigs with live cultures of Streptococcus suis type 2. Res Vet Sci. 1988 Nov;45(3):349–352. [PubMed] [Google Scholar]
  9. Holt M. E., Enright M. R., Alexander T. J. Protective effect of sera raised against different fractions of Streptococcus suis type 2. J Comp Pathol. 1990 Jul;103(1):85–94. doi: 10.1016/s0021-9975(08)80138-7. [DOI] [PubMed] [Google Scholar]
  10. Holt M. E., Enright M. R., Alexander T. J. Studies of the protective effect of different fractions of sera from pigs immune to Streptococcus suis type 2 infection. J Comp Pathol. 1989 May;100(4):435–442. doi: 10.1016/0021-9975(89)90009-1. [DOI] [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. Moxon E. R., Goldthorn J. F., Schwartz A. D. Haemophilus influenzae b infection in rats: effect of splenectomy on bloodstream and meningeal invasion after intravenous and intranasal inoculations. Infect Immun. 1980 Mar;27(3):872–875. doi: 10.1128/iai.27.3.872-875.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Moxon E. R., Ostrow P. T. Haemophilus influenzae meningitis in infant rats: role of bacteremia in pathogenesis of age-dependent inflammatory responses in cerebrospinal fluid. J Infect Dis. 1977 Feb;135(2):303–307. doi: 10.1093/infdis/135.2.303. [DOI] [PubMed] [Google Scholar]
  14. Quessy S., Dubreuil J. D., Caya M., Higgins R. Discrimination of virulent and avirulent Streptococcus suis capsular type 2 isolates from different geographical origins. Infect Immun. 1995 May;63(5):1975–1979. doi: 10.1128/iai.63.5.1975-1979.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Quessy S., Dubreuil J. D., Caya M., Létourneau R., Higgins R. Comparison of pig, rabbit and mouse IgG response to Streptococcus suis serotype 2 proteins and active immunization of mice against the infection. Can J Vet Res. 1994 Jul;58(3):220–223. [PMC free article] [PubMed] [Google Scholar]
  16. Quessy S., Dubreuil J. D., Higgins R. Immunization of mice against Streptococcus suis serotype 2 infections using a live avirulent strain. Can J Vet Res. 1994 Oct;58(4):299–301. [PMC free article] [PubMed] [Google Scholar]
  17. Quessy S., Dubreuil J. D., Jacques M., Malouin F., Higgins R. Increase of capsular material thickness following in vivo growth of virulent Streptococcus suis serotype 2 strains. FEMS Microbiol Lett. 1994 Jan 1;115(1):19–26. doi: 10.1111/j.1574-6968.1994.tb06608.x. [DOI] [PubMed] [Google Scholar]
  18. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Vecht U., Arends J. P., van der Molen E. J., van Leengoed L. A. Differences in virulence between two strains of Streptococcus suis type II after experimentally induced infection of newborn germ-free pigs. Am J Vet Res. 1989 Jul;50(7):1037–1043. [PubMed] [Google Scholar]
  20. Wessman G. E. Biology of the group E streptococci: a review. Vet Microbiol. 1986 Nov;12(4):297–328. doi: 10.1016/0378-1135(86)90081-7. [DOI] [PubMed] [Google Scholar]
  21. Williams A. E. Relationship between intracellular survival in macrophages and pathogenicity of Streptococcus suis type 2 isolates. Microb Pathog. 1990 Mar;8(3):189–196. doi: 10.1016/0882-4010(90)90046-s. [DOI] [PubMed] [Google Scholar]

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