Skip to main content
NCBI home page
Canadian Journal of Veterinary Research logoLink to Canadian Journal of Veterinary Research
. 1998 Apr;62(2):152–155.

A monoclonal antibody-based immunoperoxidase monolayer (micro-isolation) assay for detection of type 1 and type 2 bovine viral diarrhea viruses.

D Deregt 1, S Prins 1
PMCID: PMC1189464  PMID: 9553717

Abstract

A monoclonal antibody (mAb)-based immunoperoxidase monolayer assay (IPMA) for detection of bovine viral diarrhea virus (BVDV) was developed and compared with an existing bovine polyclonal antibody (pAb)-based IPMA. A pool of 5 mAbs, 4 mAbs produced to a type 1 BVDV and 1 mAb produced to a type 2 BVDV, was utilized in the mAb-IPMA. The mAbs were chosen for inclusion in the pool because of their broad cross-reactivities with type 1 and/or type 2 BVDV, their apparent avidities for antigen, their reactivity to different BVDV proteins, and their lack of competition for binding sites or their binding to unusual BVDV isolates. The mAb-IPMA outperformed the pAb-IPMA in staining, ease of reading test results, and relative sensitivity with a panel of known BVDV positive and negative sera. The relative sensitivities of the mAb-IPMA and pAb-IPMA were 100% and 93.5%, respectively, for 62 positive samples including several that were known to contain type 2 BVDV. With retesting, the pAb-IPMA gave a similar level of sensitivity as that of the mAb-IPMA. Both tests gave a specificity of 100% for 40 negative serum samples obtained from a BVDV-free herd.

Full text

PDF
152

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Afshar A., Dulac G. C., Bouffard A. Application of peroxidase labelled antibody assays for detection of porcine IgG antibodies to hog cholera and bovine viral diarrhea viruses. J Virol Methods. 1989 Mar;23(3):253–261. doi: 10.1016/0166-0934(89)90158-4. [DOI] [PubMed] [Google Scholar]
  2. Crevat D., Vandenbergh D., Chappuis G., Lecomte C., Renard A. Five hours to identify immunotolerant cattle, persistently infected with bovine virus diarrhoea virus. Rev Sci Tech. 1993 Jun;12(2):483–492. doi: 10.20506/rst.12.2.707. [DOI] [PubMed] [Google Scholar]
  3. Deregt D., Bolin S. R., Heckert R. A., Loewen K. G. Monoclonal antibodies to bovine viral diarrhea virus: cross-reactivities to field isolates and hog cholera virus strains. Can J Vet Res. 1994 Jan;58(1):71–74. [PMC free article] [PubMed] [Google Scholar]
  4. Deregt D., Loewen K. G. Bovine viral diarrhea virus: biotypes and disease. Can Vet J. 1995 Jun;36(6):371–378. [PMC free article] [PubMed] [Google Scholar]
  5. Deregt D., Masri S. A., Cho H. J., Bielefeldt Ohmann H. Monoclonal antibodies to the p80/125 gp53 proteins of bovine viral diarrhea virus: their potential use as diagnostic reagents. Can J Vet Res. 1990 Jun;54(3):343–348. [PMC free article] [PubMed] [Google Scholar]
  6. Deregt D., Masri S. A., Cho H. J., Ohmann H. B. Bovine viral diarrhea virus proteins: relatedness of p175 with p80 and p125 and evidence of glycoprotein processing. Can J Microbiol. 1991 Nov;37(11):815–822. doi: 10.1139/m91-141. [DOI] [PubMed] [Google Scholar]
  7. GILLESPIE J. H., BAKER J. A., McENTEE K. A cytopathogenic strain of virus diarrhea virus. Cornell Vet. 1960 Jan;50:73–79. [PubMed] [Google Scholar]
  8. LEE K. M., GILLESPIE J. H. Propagation of virus diarrhea virus of cattle in tissue culture. Am J Vet Res. 1957 Oct;18(69):952–953. [PubMed] [Google Scholar]
  9. Pellerin C., van den Hurk J., Lecomte J., Tijssen P. Identification of a new group of bovine viral diarrhea virus strains associated with severe outbreaks and high mortalities. Virology. 1994 Sep;203(2):260–268. doi: 10.1006/viro.1994.1483. [DOI] [PubMed] [Google Scholar]
  10. Radostits O. M., Littlejohns I. R. New concepts in the pathogenesis, diagnosis and control of diseases caused by the bovine viral diarrhea virus. Can Vet J. 1988 Jun;29(6):513–528. [PMC free article] [PubMed] [Google Scholar]
  11. Ridpath J. F., Bolin S. R. Delayed onset postvaccinal mucosal disease as a result of genetic recombination between genotype 1 and genotype 2 BVDV. Virology. 1995 Sep 10;212(1):259–262. doi: 10.1006/viro.1995.1480. [DOI] [PubMed] [Google Scholar]
  12. Ridpath J. F., Bolin S. R., Dubovi E. J. Segregation of bovine viral diarrhea virus into genotypes. Virology. 1994 Nov 15;205(1):66–74. doi: 10.1006/viro.1994.1620. [DOI] [PubMed] [Google Scholar]
  13. Ridpath J. F., Bolin S. R., Katz J. Comparison of nucleic acid hybridization and nucleic acid amplification using conserved sequences from the 5' noncoding region for detection of bovine viral diarrhea virus. J Clin Microbiol. 1993 Apr;31(4):986–989. doi: 10.1128/jcm.31.4.986-989.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sullivan D. G., Akkina R. K. A nested polymerase chain reaction assay to differentiate pestiviruses. Virus Res. 1995 Oct;38(2-3):231–239. doi: 10.1016/0168-1702(95)00065-x. [DOI] [PubMed] [Google Scholar]

Articles from Canadian Journal of Veterinary Research are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES