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Canadian Journal of Veterinary Research logoLink to Canadian Journal of Veterinary Research
. 1998 Jul;62(3):199–204.

Blood vessel density in canine osteosarcoma.

B L Coomber 1, J Denton 1, A Sylvestre 1, S Kruth 1
PMCID: PMC1189476  PMID: 9684049

Abstract

Canine osteosarcoma is a prevalent bone neoplasm which has similarities to the human disease. We used a retrospective study to investigate the possibility that tumor vascularity may provide useful prognostic information, indicative of the role of this parameter in progression of this cancer. We quantified microvessel density in 52 histological specimens of primary tumor, immunostained for von Willebrand's Factor to identify vascular endothelium. For the 20 cases not euthanized at presentation or lost to follow-up, we found significantly higher tumor microvascular densities in animals presenting with detectable pulmonary metastases (5 of 20), and significantly lower densities in animals without metastatic disease at presentation, but later surviving to develop pulmonary metastases (7 of 20; P < 0.05). Animals with no evidence of pulmonary metastases at time of death (8 of 20) had intermediate vascular densities in their tumors. The results of this preliminary study suggest that vascularity of the primary tumor may be an indication of tumor progression. Future studies with a larger number of cases should establish whether vascular density can be a useful prognostic parameter for canine osteosarcoma.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bochner B. H., Cote R. J., Weidner N., Groshen S., Chen S. C., Skinner D. G., Nichols P. W. Angiogenesis in bladder cancer: relationship between microvessel density and tumor prognosis. J Natl Cancer Inst. 1995 Nov 1;87(21):1603–1612. doi: 10.1093/jnci/87.21.1603. [DOI] [PubMed] [Google Scholar]
  2. Flanders J. A. Surgical therapy of the thyroid. Vet Clin North Am Small Anim Pract. 1994 May;24(3):607–621. doi: 10.1016/s0195-5616(94)50062-7. [DOI] [PubMed] [Google Scholar]
  3. Folkman J. Angiogenesis in cancer, vascular, rheumatoid and other disease. Nat Med. 1995 Jan;1(1):27–31. doi: 10.1038/nm0195-27. [DOI] [PubMed] [Google Scholar]
  4. Fox S. B., Turner G. D., Leek R. D., Whitehouse R. M., Gatter K. C., Harris A. L. The prognostic value of quantitative angiogenesis in breast cancer and role of adhesion molecule expression in tumor endothelium. Breast Cancer Res Treat. 1995;36(2):219–226. doi: 10.1007/BF00666042. [DOI] [PubMed] [Google Scholar]
  5. Hahn K. A., Bravo L., Adams W. H., Frazier D. L. Naturally occurring tumors in dogs as comparative models for cancer therapy research. In Vivo. 1994 Jan-Feb;8(1):133–143. [PubMed] [Google Scholar]
  6. Hanahan D., Folkman J. Patterns and emerging mechanisms of the angiogenic switch during tumorigenesis. Cell. 1996 Aug 9;86(3):353–364. doi: 10.1016/s0092-8674(00)80108-7. [DOI] [PubMed] [Google Scholar]
  7. Hayes D. F. Angiogenesis and breast cancer. Hematol Oncol Clin North Am. 1994 Feb;8(1):51–71. [PubMed] [Google Scholar]
  8. Heyman S. J., Diefenderfer D. L., Goldschmidt M. H., Newton C. D. Canine axial skeletal osteosarcoma. A retrospective study of 116 cases (1986 to 1989). Vet Surg. 1992 Jul-Aug;21(4):304–310. doi: 10.1111/j.1532-950x.1992.tb00069.x. [DOI] [PubMed] [Google Scholar]
  9. Holmgren L., O'Reilly M. S., Folkman J. Dormancy of micrometastases: balanced proliferation and apoptosis in the presence of angiogenesis suppression. Nat Med. 1995 Feb;1(2):149–153. doi: 10.1038/nm0295-149. [DOI] [PubMed] [Google Scholar]
  10. Ito T., Kitamura H., Nakamura N., Kameda Y., Kanisawa M. A comparative study of vascular proliferation in brain metastasis of lung carcinomas. Virchows Arch A Pathol Anat Histopathol. 1993;423(1):13–17. doi: 10.1007/BF01606426. [DOI] [PubMed] [Google Scholar]
  11. Kirpensteijn J. Current veterinary therapy in canine osteosarcoma. Vet Q. 1994 Apr;16 (Suppl 1):24S–25S. doi: 10.1080/01652176.1994.9694469. [DOI] [PubMed] [Google Scholar]
  12. Kurzman I. D., MacEwen E. G., Rosenthal R. C., Fox L. E., Keller E. T., Helfand S. C., Vail D. M., Dubielzig R. R., Madewell B. R., Rodriguez C. O., Jr Adjuvant therapy for osteosarcoma in dogs: results of randomized clinical trials using combined liposome-encapsulated muramyl tripeptide and cisplatin. Clin Cancer Res. 1995 Dec;1(12):1595–1601. [PubMed] [Google Scholar]
  13. Lu C., Tanigawa N. Spontaneous apoptosis is inversely related to intratumoral microvessel density in gastric carcinoma. Cancer Res. 1997 Jan 15;57(2):221–224. [PubMed] [Google Scholar]
  14. MacEwen E. G. Spontaneous tumors in dogs and cats: models for the study of cancer biology and treatment. Cancer Metastasis Rev. 1990 Sep;9(2):125–136. doi: 10.1007/BF00046339. [DOI] [PubMed] [Google Scholar]
  15. Macchiarini P., Fontanini G., Hardin M. J., Squartini F., Angeletti C. A. Relation of neovascularisation to metastasis of non-small-cell lung cancer. Lancet. 1992 Jul 18;340(8812):145–146. doi: 10.1016/0140-6736(92)93217-b. [DOI] [PubMed] [Google Scholar]
  16. Maeda K., Chung Y. S., Takatsuka S., Ogawa Y., Onoda N., Sawada T., Kato Y., Nitta A., Arimoto Y., Kondo Y. Tumour angiogenesis and tumour cell proliferation as prognostic indicators in gastric carcinoma. Br J Cancer. 1995 Aug;72(2):319–323. doi: 10.1038/bjc.1995.331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. O'Brien M. G., Straw R. C., Withrow S. J., Powers B. E., Jameson V. J., Lafferty M., Ogilvie G. K., LaRue S. M. Resection of pulmonary metastases in canine osteosarcoma: 36 cases (1983-1992). Vet Surg. 1993 Mar-Apr;22(2):105–109. doi: 10.1111/j.1532-950x.1993.tb01682.x. [DOI] [PubMed] [Google Scholar]
  18. O'Reilly M. S., Holmgren L., Shing Y., Chen C., Rosenthal R. A., Moses M., Lane W. S., Cao Y., Sage E. H., Folkman J. Angiostatin: a novel angiogenesis inhibitor that mediates the suppression of metastases by a Lewis lung carcinoma. Cell. 1994 Oct 21;79(2):315–328. doi: 10.1016/0092-8674(94)90200-3. [DOI] [PubMed] [Google Scholar]
  19. Ogawa Y., Chung Y. S., Nakata B., Takatsuka S., Maeda K., Sawada T., Kato Y., Yoshikawa K., Sakurai M., Sowa M. Microvessel quantitation in invasive breast cancer by staining for factor VIII-related antigen. Br J Cancer. 1995 Jun;71(6):1297–1301. doi: 10.1038/bjc.1995.251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ogilvie G. K., Obradovich J. E., Elmslie R. E., Vail D. M., Moore A. S., Straw R. C., Dickinson K., Cooper M. F., Withrow S. J. Efficacy of mitoxantrone against various neoplasms in dogs. J Am Vet Med Assoc. 1991 May 1;198(9):1618–1621. [PubMed] [Google Scholar]
  21. Schwarz P. D., Willer R. L. Urinary bladder neoplasia in the dog and cat. Probl Vet Med. 1989 Jan-Mar;1(1):128–140. [PubMed] [Google Scholar]
  22. Takeda T., Makita T., Nakamura N., Kimizuka G. Morphologic aspects and morphogenesis of blood cysts on canine cardiac valves. Vet Pathol. 1991 Jan;28(1):16–21. doi: 10.1177/030098589102800103. [DOI] [PubMed] [Google Scholar]
  23. Tanigawa N., Amaya H., Matsumura M., Shimomatsuya T., Horiuchi T., Muraoka R., Iki M. Extent of tumor vascularization correlates with prognosis and hematogenous metastasis in gastric carcinomas. Cancer Res. 1996 Jun 1;56(11):2671–2676. [PubMed] [Google Scholar]
  24. Vacca A., Ribatti D., Roncali L., Lospalluti M., Serio G., Carrel S., Dammacco F. Melanocyte tumor progression is associated with changes in angiogenesis and expression of the 67-kilodalton laminin receptor. Cancer. 1993 Jul 15;72(2):455–461. doi: 10.1002/1097-0142(19930715)72:2<455::aid-cncr2820720222>3.0.co;2-8. [DOI] [PubMed] [Google Scholar]
  25. Wang Z. G., Du W., Li G. D., Pu L. Q., Sharefkin J. B. Rapid cellular luminal coverage of Dacron inferior vena cava prostheses in dogs by immediate seeding of autogenous endothelial cells derived from omental tissue: results of a preliminary trial. J Vasc Surg. 1990 Aug;12(2):168–179. [PubMed] [Google Scholar]
  26. Weidner N., Carroll P. R., Flax J., Blumenfeld W., Folkman J. Tumor angiogenesis correlates with metastasis in invasive prostate carcinoma. Am J Pathol. 1993 Aug;143(2):401–409. [PMC free article] [PubMed] [Google Scholar]
  27. Weidner N. Current pathologic methods for measuring intratumoral microvessel density within breast carcinoma and other solid tumors. Breast Cancer Res Treat. 1995;36(2):169–180. doi: 10.1007/BF00666038. [DOI] [PubMed] [Google Scholar]
  28. Weidner N., Folkman J., Pozza F., Bevilacqua P., Allred E. N., Moore D. H., Meli S., Gasparini G. Tumor angiogenesis: a new significant and independent prognostic indicator in early-stage breast carcinoma. J Natl Cancer Inst. 1992 Dec 16;84(24):1875–1887. doi: 10.1093/jnci/84.24.1875. [DOI] [PubMed] [Google Scholar]
  29. Weidner N. Intratumor microvessel density as a prognostic factor in cancer. Am J Pathol. 1995 Jul;147(1):9–19. [PMC free article] [PubMed] [Google Scholar]
  30. Weidner N., Semple J. P., Welch W. R., Folkman J. Tumor angiogenesis and metastasis--correlation in invasive breast carcinoma. N Engl J Med. 1991 Jan 3;324(1):1–8. doi: 10.1056/NEJM199101033240101. [DOI] [PubMed] [Google Scholar]
  31. Withrow S. J., Powers B. E., Straw R. C., Wilkins R. M. Comparative aspects of osteosarcoma. Dog versus man. Clin Orthop Relat Res. 1991 Sep;(270):159–168. [PubMed] [Google Scholar]
  32. Withrow S. J., Thrall D. E., Straw R. C., Powers B. E., Wrigley R. H., Larue S. M., Page R. L., Richardson D. C., Bissonette K. W., Betts C. W. Intra-arterial cisplatin with or without radiation in limb-sparing for canine osteosarcoma. Cancer. 1993 Apr 15;71(8):2484–2490. doi: 10.1002/1097-0142(19930415)71:8<2484::aid-cncr2820710810>3.0.co;2-d. [DOI] [PubMed] [Google Scholar]
  33. von Beust B. R., Suter M. M., Summers B. A. Factor VIII-related antigen in canine endothelial neoplasms: an immunohistochemical study. Vet Pathol. 1988 Jul;25(4):251–255. doi: 10.1177/030098588802500401. [DOI] [PubMed] [Google Scholar]

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