Skip to main content
NCBI home page
Canadian Journal of Veterinary Research logoLink to Canadian Journal of Veterinary Research
. 2001 Jan;65(1):45–49.

Preliminary studies of a canine 13C-aminopyrine demethylation blood test.

E M Moeller 1, J M Steiner 1, D A Williams 1, P D Klein 1
PMCID: PMC1189641  PMID: 11227194

Abstract

The objectives of this study were to determine whether a 13C-aminopyrine demethylation blood test is technically feasible in clinically healthy dogs, whether oral administration of 13C-aminopyrine causes a detectable increase in percent dose/min (PCD) of 13C administered as 13C-aminopyrine and recovered in gas extracted from blood, and whether gas extraction efficiency has an impact on PCD. A dose of 2 mg/kg body weight of 13C-aminopyrine dissolved in deionized water was administered orally to 6 clinically healthy dogs. Blood samples were taken from each dog 0, 30, 60, and 120 min after administration of the 13C-aminopyrine. Carbon dioxide was extracted from blood samples by addition of acid and analyzed by fractional mass spectrometry. None of the 6 dogs showed any side effects after 13C-aminopyrine administration. All 6 dogs showed a measurable increase of the PCD in gas samples extracted from blood samples at 30 min, 60 min, and 120 min after 13C-aminopyrine administration. Coefficients of variation between the triplicate samples were statistically significantly higher for the %CO2, a measure of extraction efficiency, than for PCD values (P < 0.0001). The 13C-aminopyrine demethylation blood test described here is technically feasible. Oral administration of 13C-aminopyrine did not lead to gross side effects in the 6 dogs. Clinically healthy dogs show a measurable increase of PCD in gas extracted from blood samples after oral administration of 13C-aminopyrine. Efficiency of CO2 extraction from blood samples does not have an impact on PCD determined from these blood samples. This test may prove useful to evaluate hepatic function in dogs.

Full text

PDF
45

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker A. L., Krager P. S., Kotake A. N., Schoeller D. A. The aminopyrine breath test does not correlate with histologic disease severity in patients with cholestasis. Hepatology. 1987 May-Jun;7(3):464–467. doi: 10.1002/hep.1840070309. [DOI] [PubMed] [Google Scholar]
  2. Beyeler C., Reichen J., Thomann S. R., Lauterburg B. H., Gerber N. J. Quantitative liver function in patients with rheumatoid arthritis treated with low-dose methotrexate: a longitudinal study. Br J Rheumatol. 1997 Mar;36(3):338–344. doi: 10.1093/rheumatology/36.3.338. [DOI] [PubMed] [Google Scholar]
  3. Bircher J. Quantitative assessment of deranged hepatic function: a missed opportunity? Semin Liver Dis. 1983 Nov;3(4):275–284. doi: 10.1055/s-2008-1040780. [DOI] [PubMed] [Google Scholar]
  4. Boothe D. M., Cullen J. M., Calvin J. A., Jenkins W. L., Brown S. A., Green R. A., Corrier D. E. Antipyrine and caffeine dispositions in clinically normal dogs and dogs with progressive liver disease. Am J Vet Res. 1994 Feb;55(2):254–261. [PubMed] [Google Scholar]
  5. Center S. A., Bunch S. E., Baldwin B. H., Hornbuckle W. E., Tennant B. C. Comparison of sulfobromophthalein and indocyanine green clearances in the dog. Am J Vet Res. 1983 Apr;44(4):722–726. [PubMed] [Google Scholar]
  6. Chey W. D., Murthy U., Toskes P., Carpenter S., Laine L. The 13C-urea blood test accurately detects active Helicobacter pylori infection: a United States, multicenter trial. Am J Gastroenterol. 1999 Jun;94(6):1522–1524. doi: 10.1111/j.1572-0241.1999.1137_r.x. [DOI] [PubMed] [Google Scholar]
  7. Cornetta A. M., Simpson K. W., Strauss-Ayali D., McDonough P. L., Gleed R. D. Use of a [13C]urea breath test for detection of gastric infection with Helicobacter spp in dogs. Am J Vet Res. 1998 Nov;59(11):1364–1369. [PubMed] [Google Scholar]
  8. Cutler A. F., Toskes P. Comparison of [13C]urea blood test to [13C]urea breath test for the diagnosis of Helicobacter pylori. Am J Gastroenterol. 1999 Apr;94(4):959–961. doi: 10.1111/j.1572-0241.1999.994_q.x. [DOI] [PubMed] [Google Scholar]
  9. Gikalov I., Bircher J. Dose dependence of the 14C-aminopyrine breath test. Intrasubject comparison of tracer and pharmacological doses. Eur J Clin Pharmacol. 1977 Nov 14;12(3):229–233. doi: 10.1007/BF00609866. [DOI] [PubMed] [Google Scholar]
  10. Henry D. A., Kitchingman G., Langman M. J. [14C]Aminopyrine breath analysis and conventional biochemical tests as predictors of survival in cirrhosis. Dig Dis Sci. 1985 Sep;30(9):813–818. doi: 10.1007/BF01309509. [DOI] [PubMed] [Google Scholar]
  11. Hoffmann W. E., Renegar W. E., Dorner J. L. Serum half-life of intravenously injected intestinal and hepatic alkaline phosphatase isoenzymes in the cat. Am J Vet Res. 1977 Oct;38(10):1637–1639. [PubMed] [Google Scholar]
  12. Hofmann A. F., Lauterburg B. H. Breath test with isotopes of carbon: progress and potential. J Lab Clin Med. 1977 Sep;90(3):405–411. [PubMed] [Google Scholar]
  13. Kessler H. A., Bick J. A., Pottage J. C., Jr, Benson C. A. AIDS: Part I. Dis Mon. 1992 Sep;38(9):633–690. doi: 10.1016/0011-5029(92)90029-o. [DOI] [PubMed] [Google Scholar]
  14. Miotti T., Bircher J., Preisig R. The 30-minute aminopyrine breath test: optimization of sampling times after intravenous administration of 14C-aminopyrine. Digestion. 1988;39(4):241–250. doi: 10.1159/000199632. [DOI] [PubMed] [Google Scholar]
  15. Schneider J. F., Baker A. L., Haines N. W., Hatfield G., Boyer J. L. Aminopyrine N-demethylation: a prognostic test of liver function in patients with alcoholic liver disease. Gastroenterology. 1980 Dec;79(6):1145–1150. [PubMed] [Google Scholar]
  16. Schoeller D. A., Kotake A. N., Lambert G. H., Krager P. S., Baker A. L. Comparison of the phenacetin and aminopyrine breath tests: effect of liver disease, inducers and cobaltous chloride. Hepatology. 1985 Mar-Apr;5(2):276–281. doi: 10.1002/hep.1840050221. [DOI] [PubMed] [Google Scholar]
  17. Villeneuve J. P., Infante-Rivard C., Ampelas M., Pomier-Layrargues G., Huet P. M., Marleau D. Prognostic value of the aminopyrine breath test in cirrhotic patients. Hepatology. 1986 Sep-Oct;6(5):928–931. doi: 10.1002/hep.1840060520. [DOI] [PubMed] [Google Scholar]

Articles from Canadian Journal of Veterinary Research are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES