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. 2001 Oct;65(4):229–232.

The importance of interleukin-8 as a neutrophil chemoattractant in the lungs of cattle with pneumonic pasteurellosis.

J L Caswell 1, D M Middleton 1, J R Gordon 1
PMCID: PMC1189684  PMID: 11768129

Abstract

Interleukin-8 (IL-8), an in vitro and in vivo neutrophil chemoattractant, is expressed at high levels in the lesions observed in bovine pneumonic pasteurellosis. Because of the role of neutrophils in the pathogenesis of pneumonic pasteurellosis, we investigated the relative importance of IL-8 as a neutrophil chemoattractant in this disease. Bronchoalveolar lavage (BAL) fluid was harvested from calves experimentally infected with bovine herpesvirus-1 and challenged with Mannheimia haemolytica. Neutrophil chemotactic activity was measured in pneumonic BAL fluid samples treated with a neutralizing monoclonal antibody to ovine IL-8, and compared to the activity in samples treated with an isotype-matched control antibody. Bronchoalveolar lavage fluid was analyzed at a dilution which induced a half-maximal response, and the concentrations of antibody were optimized in a preliminary experiment. Following incubation of replicate samples of diluted pneumonic bovine BAL fluid with 70 microg/mL of IL-8-neutralizing antibody or control antibody, the neutrophil chemotactic activities of the samples were determined using an in vitro microchemotaxis assay. Overall, pretreatment of BAL fluid samples with neutralizing anti-IL-8 antibody reduced neutrophil chemotactic activity by 15% to 60%, compared to pretreatment with control antibody. This effect was highly significant (P < 0.001), and was present in 5 of 5 samples. These data indicate that IL-8 is an important neutrophil chemoattractant in calves with pneumonic pasteurellosis, but that mediators with actions redundant to those of IL-8 must also be present in the lesions.

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Selected References

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  1. Ahuja S. K., Murphy P. M. The CXC chemokines growth-regulated oncogene (GRO) alpha, GRObeta, GROgamma, neutrophil-activating peptide-2, and epithelial cell-derived neutrophil-activating peptide-78 are potent agonists for the type B, but not the type A, human interleukin-8 receptor. J Biol Chem. 1996 Aug 23;271(34):20545–20550. doi: 10.1074/jbc.271.34.20545. [DOI] [PubMed] [Google Scholar]
  2. Allmann-Iselin I., Car B. D., Zwahlen R. D., Mueller-Schüpbach R., Wyder-Walther M., Steckholzer U., Walz A. Bovine ENA, a new monocyte-macrophage derived cytokine of the interleukin-8 family. Structure, function, and expression in acute pulmonary inflammation. Am J Pathol. 1994 Dec;145(6):1382–1389. [PMC free article] [PubMed] [Google Scholar]
  3. Beaubien B. C., Collins P. D., Jose P. J., Totty N. F., Hsuan J., Waterfield M. D., Williams T. J. A novel neutrophil chemoattractant generated during an inflammatory reaction in the rabbit peritoneal cavity in vivo. Purification, partial amino acid sequence and structural relationship to interleukin 8. Biochem J. 1990 Nov 1;271(3):797–801. doi: 10.1042/bj2710797. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bochsler P. N., Neilsen N. R., Slauson D. O. Transendothelial migration of neonatal and adult bovine neutrophils in vitro. J Leukoc Biol. 1994 Jan;55(1):43–49. doi: 10.1002/jlb.55.1.43. [DOI] [PubMed] [Google Scholar]
  5. Breider M. A., Walker R. D., Hopkins F. M., Schultz T. W., Bowersock T. L. Pulmonary lesions induced by Pasteurella haemolytica in neutrophil sufficient and neutrophil deficient calves. Can J Vet Res. 1988 Apr;52(2):205–209. [PMC free article] [PubMed] [Google Scholar]
  6. Broaddus V. C., Boylan A. M., Hoeffel J. M., Kim K. J., Sadick M., Chuntharapai A., Hébert C. A. Neutralization of IL-8 inhibits neutrophil influx in a rabbit model of endotoxin-induced pleurisy. J Immunol. 1994 Mar 15;152(6):2960–2967. [PubMed] [Google Scholar]
  7. Broaddus V. C., Hébert C. A., Vitangcol R. V., Hoeffel J. M., Bernstein M. S., Boylan A. M. Interleukin-8 is a major neutrophil chemotactic factor in pleural liquid of patients with empyema. Am Rev Respir Dis. 1992 Oct;146(4):825–830. doi: 10.1164/ajrccm/146.4.825. [DOI] [PubMed] [Google Scholar]
  8. Cassatella M. A. The production of cytokines by polymorphonuclear neutrophils. Immunol Today. 1995 Jan;16(1):21–26. doi: 10.1016/0167-5699(95)80066-2. [DOI] [PubMed] [Google Scholar]
  9. Caswell J. L., Middleton D. M., Gordon J. R. Production and functional characterization of recombinant bovine interleukin-8 as a specific neutrophil activator and chemoattractant. Vet Immunol Immunopathol. 1999 Mar 1;67(4):327–340. doi: 10.1016/s0165-2427(99)00007-0. [DOI] [PubMed] [Google Scholar]
  10. Caswell J. L., Middleton D. M., Sorden S. D., Gordon J. R. Expression of the neutrophil chemoattractant interleukin-8 in the lesions of bovine pneumonic pasteurellosis. Vet Pathol. 1998 Mar;35(2):124–131. doi: 10.1177/030098589803500206. [DOI] [PubMed] [Google Scholar]
  11. Forsell J. H., Kateley J. R., Smith C. W. Bovine neutrophils treated with chemotactic agents: morphologic changes. Am J Vet Res. 1985 Sep;46(9):1971–1974. [PubMed] [Google Scholar]
  12. Godson D. L., Campos M., Attah-Poku S. K., Redmond M. J., Cordeiro D. M., Sethi M. S., Harland R. J., Babiuk L. A. Serum haptoglobin as an indicator of the acute phase response in bovine respiratory disease. Vet Immunol Immunopathol. 1996 Jun 1;51(3-4):277–292. doi: 10.1016/0165-2427(95)05520-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gray G. D., Knight K. A., Nelson R. D., Herron M. J. Chemotactic requirements of bovine leukocytes. Am J Vet Res. 1982 May;43(5):757–759. [PubMed] [Google Scholar]
  14. Hassfurther R. L., Canning P. C., Geib R. W. Isolation and characterization of an interleukin-8-like peptide in the bovine species. Vet Immunol Immunopathol. 1994 Aug;42(2):117–126. doi: 10.1016/0165-2427(94)90001-9. [DOI] [PubMed] [Google Scholar]
  15. Hauser C. J., Fekete Z., Goodman E. R., Kleinstein E., Livingston D. H., Deitch E. A. CXCR2 stimulation primes CXCR1 [Ca2+]i responses to IL-8 in human neutrophils. Shock. 1999 Dec;12(6):428–437. doi: 10.1097/00024382-199912000-00003. [DOI] [PubMed] [Google Scholar]
  16. Lindley I., Aschauer H., Seifert J. M., Lam C., Brunowsky W., Kownatzki E., Thelen M., Peveri P., Dewald B., von Tscharner V. Synthesis and expression in Escherichia coli of the gene encoding monocyte-derived neutrophil-activating factor: biological equivalence between natural and recombinant neutrophil-activating factor. Proc Natl Acad Sci U S A. 1988 Dec;85(23):9199–9203. doi: 10.1073/pnas.85.23.9199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Malazdrewich C., Ames T. R., Abrahamsen M. S., Maheswaran S. K. Pulmonary expression of tumor necrosis factor alpha, interleukin-1 beta, and interleukin-8 in the acute phase of bovine pneumonic pasteurellosis. Vet Pathol. 2001 May;38(3):297–310. doi: 10.1354/vp.38-3-297. [DOI] [PubMed] [Google Scholar]
  18. Morsey M. A., Van-Kessel A. G., Mori Y., Popowych Y., Godson D., Campos M., Babiuk L. A. Cytokine profiles following interaction between bovine alveolar macrophages and Pasteurella haemolytica. Microb Pathog. 1999 Jun;26(6):325–331. doi: 10.1006/mpat.1999.0274. [DOI] [PubMed] [Google Scholar]
  19. Persson K., Larsson I., Hallén Sandgren C. Effects of certain inflammatory mediators on bovine neutrophil migration in vivo and in vitro. Vet Immunol Immunopathol. 1993 Jul;37(2):99–112. doi: 10.1016/0165-2427(93)90058-c. [DOI] [PubMed] [Google Scholar]
  20. Rogivue C., Car B. D., Allmann-Iselin I., Zwahlen R. D., Walz A. Bovine melanoma growth stimulatory activity: a new monocyte-macrophage-derived cytokine of the IL-8 family. Partial structure, function, and expression in acute pulmonary inflammation. Lab Invest. 1995 Jun;72(6):689–695. [PubMed] [Google Scholar]
  21. Seow H. F., Yoshimura T., Wood P. R., Colditz I. G. Cloning, sequencing, expression and inflammatory activity in skin of ovine interleukin-8. Immunol Cell Biol. 1994 Oct;72(5):398–405. doi: 10.1038/icb.1994.59. [DOI] [PubMed] [Google Scholar]
  22. Slocombe R. F., Malark J., Ingersoll R., Derksen F. J., Robinson N. E. Importance of neutrophils in the pathogenesis of acute pneumonic pasteurellosis in calves. Am J Vet Res. 1985 Nov;46(11):2253–2258. [PubMed] [Google Scholar]
  23. Weiss D. J., Bauer M. C., Whiteley L. O., Maheswaran S. K., Ames T. R. Changes in blood and bronchoalveolar lavage fluid components in calves with experimentally induced pneumonic pasteurellosis. Am J Vet Res. 1991 Feb;52(2):337–344. [PubMed] [Google Scholar]
  24. Wilkinson P. C. Micropore filter methods for leukocyte chemotaxis. Methods Enzymol. 1988;162:38–50. doi: 10.1016/0076-6879(88)62061-1. [DOI] [PubMed] [Google Scholar]
  25. Yokoi K., Mukaida N., Harada A., Watanabe Y., Matsushima K. Prevention of endotoxemia-induced acute respiratory distress syndrome-like lung injury in rabbits by a monoclonal antibody to IL-8. Lab Invest. 1997 Mar;76(3):375–384. [PubMed] [Google Scholar]

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