Abstract
1. Bull-frog sympathetic neurones in primary culture were voltage clamped in the whole-cell configuration. The pipette solution contained ATP (5 mM). 2. A hyperpolarization-activated sodium-potassium current (H-current: IH) was separated from other membrane currents in a nominally calcium-free solution containing cobalt (2 mM), magnesium (4 mM), barium (2 mM), tetraethylammonium (20 mM), tetrodotoxin (3 microM), apamin (30 nM) and 4-aminopyridine (1 mM). IH was selectively blocked by caesium (10-300 microM). 3. The steady-state activation of IH occurred between -60 and -130 mV. The H-conductance was 4.1-6.6 nS at the half-activation voltage of -90 mV. With the concentrations of potassium and sodium ions in the superfusate at 20 and 70 mM, respectively, the reversal potential of IH was about -20 mV. IH was activated with a time constant of 2.8 s at -90 mV and 22 degrees C. The Q10 between 16 and 26 degrees C was 4.3. 4. A non-hydrolysable ATP analogue in the pipette solution did not support IH activation. Intracellular 'loading' of GTP-gamma-S (30-500 microM) led to a progressive activation of IH. 5. Forskolin (10 microM) increased the maximum conductance of IH by 70%. This was associated with a depolarizing shift in the half-activation voltage (5-10 mV) and in the voltage dependence of the activation/deactivation time constant of IH. 6. Essentially the same results as with forskolin were obtained by intracellular 'loading' with cyclic AMP (3-10 microM) or bath application of 8-bromo cyclic AMP (0.1-1 mM), dibutyryl cyclic AMP (1 mM) and 3-isobutyl-1-methylxanthine (0.1-1 mM). 7. The protein kinase inhibitor H-8 (1-10 microM) decreased the peak amplitude of IH. Phorbol 12-myristate 13-acetate (10 microM), a protein kinase C activator, was without effect. 8. It is concluded that a voltage-dependent cation current can be regulated by the basal activity of adenylate cyclase, presumably through protein kinase A, in vertebrate sympathetic neurones.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Adams P. R., Brown D. A., Constanti A. M-currents and other potassium currents in bullfrog sympathetic neurones. J Physiol. 1982 Sep;330:537–572. doi: 10.1113/jphysiol.1982.sp014357. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Adams P. R., Brown D. A., Constanti A. Pharmacological inhibition of the M-current. J Physiol. 1982 Nov;332:223–262. doi: 10.1113/jphysiol.1982.sp014411. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Akasu T. Adrenaline depolarization in paravertebral sympathetic neurones of bullfrogs. Pflugers Arch. 1988 Jan;411(1):80–87. doi: 10.1007/BF00581650. [DOI] [PubMed] [Google Scholar]
- Akasu T. Adrenaline inhibits muscarinic transmission in bullfrog sympathetic ganglia. Pflugers Arch. 1989 Apr;413(6):616–621. doi: 10.1007/BF00581811. [DOI] [PubMed] [Google Scholar]
- Akasu T., Hirai K., Koketsu K. Modulatory actions of ATP on membrane potentials of bullfrog sympathetic ganglion cells. Brain Res. 1983 Jan 10;258(2):313–317. doi: 10.1016/0006-8993(83)91157-5. [DOI] [PubMed] [Google Scholar]
- Barrett E. F., Barrett J. N., Crill W. E. Voltage-sensitive outward currents in cat motoneurones. J Physiol. 1980 Jul;304:251–276. doi: 10.1113/jphysiol.1980.sp013323. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Benham C. D., Bolton T. B., Denbigh J. S., Lang R. J. Inward rectification in freshly isolated single smooth muscle cells of the rabbit jejunum. J Physiol. 1987 Feb;383:461–476. doi: 10.1113/jphysiol.1987.sp016421. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brown D. M-currents: an update. Trends Neurosci. 1988 Jul;11(7):294–299. doi: 10.1016/0166-2236(88)90089-6. [DOI] [PubMed] [Google Scholar]
- Castagna M., Takai Y., Kaibuchi K., Sano K., Kikkawa U., Nishizuka Y. Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters. J Biol Chem. 1982 Jul 10;257(13):7847–7851. [PubMed] [Google Scholar]
- Crepel F., Penit-Soria J. Inward rectification and low threshold calcium conductance in rat cerebellar Purkinje cells. An in vitro study. J Physiol. 1986 Mar;372:1–23. doi: 10.1113/jphysiol.1986.sp015993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DiFrancesco D. A new interpretation of the pace-maker current in calf Purkinje fibres. J Physiol. 1981 May;314:359–376. doi: 10.1113/jphysiol.1981.sp013713. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DiFrancesco D. A study of the ionic nature of the pace-maker current in calf Purkinje fibres. J Physiol. 1981 May;314:377–393. doi: 10.1113/jphysiol.1981.sp013714. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DiFrancesco D. Characterization of the pace-maker current kinetics in calf Purkinje fibres. J Physiol. 1984 Mar;348:341–367. doi: 10.1113/jphysiol.1984.sp015114. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DiFrancesco D., Ferroni A., Mazzanti M., Tromba C. Properties of the hyperpolarizing-activated current (if) in cells isolated from the rabbit sino-atrial node. J Physiol. 1986 Aug;377:61–88. doi: 10.1113/jphysiol.1986.sp016177. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DiFrancesco D., Tromba C. Acetylcholine inhibits activation of the cardiac hyperpolarizing-activated current, if. Pflugers Arch. 1987 Sep;410(1-2):139–142. doi: 10.1007/BF00581906. [DOI] [PubMed] [Google Scholar]
- DiFrancesco D., Tromba C. Inhibition of the hyperpolarization-activated current (if) induced by acetylcholine in rabbit sino-atrial node myocytes. J Physiol. 1988 Nov;405:477–491. doi: 10.1113/jphysiol.1988.sp017343. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DiFrancesco D., Tromba C. Muscarinic control of the hyperpolarization-activated current (if) in rabbit sino-atrial node myocytes. J Physiol. 1988 Nov;405:493–510. doi: 10.1113/jphysiol.1988.sp017344. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dodd J., Horn J. P. A reclassification of B and C neurones in the ninth and tenth paravertebral sympathetic ganglia of the bullfrog. J Physiol. 1983 Jan;334:255–269. doi: 10.1113/jphysiol.1983.sp014493. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dutar P., Nicoll R. A. Stimulation of phosphatidylinositol (PI) turnover may mediate the muscarinic suppression of the M-current in hippocampal pyramidal cells. Neurosci Lett. 1988 Feb 15;85(1):89–94. doi: 10.1016/0304-3940(88)90434-x. [DOI] [PubMed] [Google Scholar]
- Fox A. P., Nowycky M. C., Tsien R. W. Kinetic and pharmacological properties distinguishing three types of calcium currents in chick sensory neurones. J Physiol. 1987 Dec;394:149–172. doi: 10.1113/jphysiol.1987.sp016864. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Griffith W. H. Membrane properties of cell types within guinea pig basal forebrain nuclei in vitro. J Neurophysiol. 1988 May;59(5):1590–1612. doi: 10.1152/jn.1988.59.5.1590. [DOI] [PubMed] [Google Scholar]
- Halliwell J. V., Adams P. R. Voltage-clamp analysis of muscarinic excitation in hippocampal neurons. Brain Res. 1982 Oct 28;250(1):71–92. doi: 10.1016/0006-8993(82)90954-4. [DOI] [PubMed] [Google Scholar]
- Hidaka H., Inagaki M., Kawamoto S., Sasaki Y. Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide dependent protein kinase and protein kinase C. Biochemistry. 1984 Oct 9;23(21):5036–5041. doi: 10.1021/bi00316a032. [DOI] [PubMed] [Google Scholar]
- Jones S. W. Sodium currents in dissociated bull-frog sympathetic neurones. J Physiol. 1987 Aug;389:605–627. doi: 10.1113/jphysiol.1987.sp016674. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kuffler S. W., Sejnowski T. J. Peptidergic and muscarinic excitation at amphibian sympathetic synapses. J Physiol. 1983 Aug;341:257–278. doi: 10.1113/jphysiol.1983.sp014805. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mayer M. L. Selective block of inward but not outward rectification in rat sensory neurones infected with herpes simplex virus. J Physiol. 1986 Jun;375:327–338. doi: 10.1113/jphysiol.1986.sp016119. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mayer M. L., Westbrook G. L. A voltage-clamp analysis of inward (anomalous) rectification in mouse spinal sensory ganglion neurones. J Physiol. 1983 Jul;340:19–45. doi: 10.1113/jphysiol.1983.sp014747. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pennefather P., Lancaster B., Adams P. R., Nicoll R. A. Two distinct Ca-dependent K currents in bullfrog sympathetic ganglion cells. Proc Natl Acad Sci U S A. 1985 May;82(9):3040–3044. doi: 10.1073/pnas.82.9.3040. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pfaffinger P. J., Leibowitz M. D., Subers E. M., Nathanson N. M., Almers W., Hille B. Agonists that suppress M-current elicit phosphoinositide turnover and Ca2+ transients, but these events do not explain M-current suppression. Neuron. 1988 Aug;1(6):477–484. doi: 10.1016/0896-6273(88)90178-x. [DOI] [PubMed] [Google Scholar]
- Pfaffinger P. Muscarine and t-LHRH suppress M-current by activating an IAP-insensitive G-protein. J Neurosci. 1988 Sep;8(9):3343–3353. doi: 10.1523/JNEUROSCI.08-09-03343.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sims S. M., Singer J. J., Walsh J. V., Jr Antagonistic adrenergic-muscarinic regulation of M current in smooth muscle cells. Science. 1988 Jan 8;239(4836):190–193. doi: 10.1126/science.2827305. [DOI] [PubMed] [Google Scholar]
- Tanaka K., Kuba K. The Ca2+-sensitive K+-currents underlying the slow afterhyperpolarization of bullfrog sympathetic neurones. Pflugers Arch. 1987 Oct;410(3):234–242. doi: 10.1007/BF00580271. [DOI] [PubMed] [Google Scholar]
- Tokimasa T. Calcium-dependent hyperpolarizations in bullfrog sympathetic neurons. Neuroscience. 1984 Jul;12(3):929–937. doi: 10.1016/0306-4522(84)90180-5. [DOI] [PubMed] [Google Scholar]
- Tokimasa T. Intracellular Ca2+-ions inactivate K+-current in bullfrog sympathetic neurons. Brain Res. 1985 Jul 1;337(2):386–391. doi: 10.1016/0006-8993(85)90081-2. [DOI] [PubMed] [Google Scholar]
- Tokimasa T. Spontaneous muscarinic suppression of the Ca-activated K-current in bullfrog sympathetic neurons. Brain Res. 1985 Sep 30;344(1):134–141. doi: 10.1016/0006-8993(85)91197-7. [DOI] [PubMed] [Google Scholar]
- Tsien R. W., Giles W., Greengard P. Cyclic AMP mediates the effects of adrenaline on cardiac purkinje fibres. Nat New Biol. 1972 Dec 6;240(101):181–183. doi: 10.1038/newbio240181a0. [DOI] [PubMed] [Google Scholar]
- Tsuji S., Minota S., Kuba K. Regulation of two ion channels by a common muscarinic receptor-transduction system in a vertebrate neuron. Neurosci Lett. 1987 Oct 16;81(1-2):139–145. doi: 10.1016/0304-3940(87)90354-5. [DOI] [PubMed] [Google Scholar]
- Williams J. T., Colmers W. F., Pan Z. Z. Voltage- and ligand-activated inwardly rectifying currents in dorsal raphe neurons in vitro. J Neurosci. 1988 Sep;8(9):3499–3506. doi: 10.1523/JNEUROSCI.08-09-03499.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Williams J. T., North R. A., Tokimasa T. Inward rectification of resting and opiate-activated potassium currents in rat locus coeruleus neurons. J Neurosci. 1988 Nov;8(11):4299–4306. doi: 10.1523/JNEUROSCI.08-11-04299.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yanagihara K., Irisawa H. Inward current activated during hyperpolarization in the rabbit sinoatrial node cell. Pflugers Arch. 1980 May;385(1):11–19. doi: 10.1007/BF00583909. [DOI] [PubMed] [Google Scholar]