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. 1988 Nov;405:39–55. doi: 10.1113/jphysiol.1988.sp017320

Binding and biological actions of prostaglandin E2 and I2 in cells isolated from rabbit gastric mucosa.

D B Barr 1, J A Duncan 1, J A Kiernan 1, B D Soper 1, B L Tepperman 1
PMCID: PMC1190963  PMID: 2475608

Abstract

1. We have investigated the binding of the tritiated forms of prostaglandin E2 (PGE2) and a stable analogue of prostacyclin (Iloprost) to isolated cells of rabbit oxyntic mucosa. 2. The highest degree of specific [3H]PGE2 binding occurred in a cellular fraction enriched in parietal cells. [3H]Iloprost binding occurred predominantly in cells identified as mucous cells. 3. PGE2 binding to the parietal cell fraction was associated with its ability to inhibit histamine-stimulated aminopyrine accumulation by these cells. Iloprost binding did not correlate with a biological action on the parietal cells. 4. PGE2 and Iloprost reduced Trypan Blue staining in cells exposed to 10% (w/v) ethanol. Iloprost (10(-8) to 10(-6) M) reduced Trypan Blue staining in cells identified as mucous and parietal cells. PGE2 (10(-8) M) significantly reduced Trypan Blue staining in parietal cell-enriched fractions. 5. Cyclic AMP stimulation in response to either prostanoid occurred most potently on non-parietal cell fractions. However PGE2 or Iloprost binding affinities did not correlate with cyclic AMP formation. 6. These data provide evidence for true PGE2 receptors on oxyntic mucosal cells. The receptors appear to mediate inhibition of acid secretion. Iloprost binds to sites which might mediate cellular protection.

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Selected References

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  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  2. COOK H. C. Periodic acid-Schiff-toluidine blue-aurantia: a stain for the gland cells of the stomach. Stain Technol. 1962 Sep;37:317–319. doi: 10.3109/10520296209114493. [DOI] [PubMed] [Google Scholar]
  3. DeLean A., Munson P. J., Rodbard D. Simultaneous analysis of families of sigmoidal curves: application to bioassay, radioligand assay, and physiological dose-response curves. Am J Physiol. 1978 Aug;235(2):E97–102. doi: 10.1152/ajpendo.1978.235.2.E97. [DOI] [PubMed] [Google Scholar]
  4. McPherson G. A. A practical computer-based approach to the analysis of radioligand binding experiments. Comput Programs Biomed. 1983 Aug-Oct;17(1-2):107–113. doi: 10.1016/0010-468x(83)90031-4. [DOI] [PubMed] [Google Scholar]
  5. Miller T. A. Protective effects of prostaglandins against gastric mucosal damage: current knowledge and proposed mechanisms. Am J Physiol. 1983 Nov;245(5 Pt 1):G601–G623. doi: 10.1152/ajpgi.1983.245.5.G601. [DOI] [PubMed] [Google Scholar]
  6. Munson P. J., Rodbard D. Ligand: a versatile computerized approach for characterization of ligand-binding systems. Anal Biochem. 1980 Sep 1;107(1):220–239. doi: 10.1016/0003-2697(80)90515-1. [DOI] [PubMed] [Google Scholar]
  7. Müller-Lissner S. A., Fimmel C., Sonnenberg A., Peskar B., Fischer J. A., Blum A. L. The effect of prostaglandins on isolated rat gastric cells. Scand J Gastroenterol Suppl. 1981;67:229–232. [PubMed] [Google Scholar]
  8. Sheppard M. S., Spence J. W., Kraicer J. Release of growth hormone from purified somatotrophs: role of adenosine 3',5'-monophosphate and guanosine 3',5'-monophosphate. Endocrinology. 1979 Jul;105(1):261–268. doi: 10.1210/endo-105-1-261. [DOI] [PubMed] [Google Scholar]
  9. Skoglund M. L., Nies A. S., Gerber J. G. Inhibition of acid secretion in isolated canine parietal cells by prostaglandins. J Pharmacol Exp Ther. 1982 Feb;220(2):371–374. [PubMed] [Google Scholar]
  10. Soll A. H. Specific inhibition by prostaglandins E2 and I2 of histamine-stimulated [14C]aminopyrine accumulation and cyclic adenosine monophosphate generation by isolated canine parietal cells. J Clin Invest. 1980 May;65(5):1222–1229. doi: 10.1172/JCI109777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Soll A. H. The actions of secretagogues on oxygen uptake by isolated mammalian parietal cells. J Clin Invest. 1978 Feb;61(2):370–380. doi: 10.1172/JCI108947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Soll A. H., Whittle B. J. Interaction between prostaglandins and cyclic AMP in the gastric mucosa. Prostaglandins. 1981;21 (Suppl):39–45. doi: 10.1016/0090-6980(81)90115-5. [DOI] [PubMed] [Google Scholar]
  13. Soll A. H., Wollin A. Histamine and cyclic AMP in isolated canine parietal cells. Am J Physiol. 1979 Nov;237(5):E444–E450. doi: 10.1152/ajpendo.1979.237.5.E444. [DOI] [PubMed] [Google Scholar]
  14. Tepperman B. L., Soper B. D., Emery S. K. Specific binding of a stable prostacyclin analogue (Iloprost) to rat oxyntic mucosa. Prostaglandins. 1984 Oct;28(4):477–484. doi: 10.1016/0090-6980(84)90236-3. [DOI] [PubMed] [Google Scholar]
  15. Tepperman B. L., Soper B. D. Prostaglandin E2-binding sites and cAMP production in porcine fundic mucosa. Am J Physiol. 1981 Oct;241(4):G313–G320. doi: 10.1152/ajpgi.1981.241.4.G313. [DOI] [PubMed] [Google Scholar]
  16. Tepperman B. L., Soper B. D. Subcellular distribution of [3H]-prostaglandin E2 binding sites in porcine gastric mucosa. Prostaglandins. 1983 Mar;25(3):425–441. doi: 10.1016/0090-6980(83)90046-1. [DOI] [PubMed] [Google Scholar]
  17. Terano A., Mach T., Stachura J., Tarnawski A., Ivey K. J. Effect of 16,16 dimethyl prostaglandin E2 on aspirin induced damage to rat gastric epithelial cells in tissue culture. Gut. 1984 Jan;25(1):19–25. doi: 10.1136/gut.25.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Terano A., Ota S., Mach T., Hiraishi H., Stachura J., Tarnawski A., Ivey K. J. Prostaglandin protects against taurocholate-induced damage to rat gastric mucosal cell culture. Gastroenterology. 1987 Mar;92(3):669–677. doi: 10.1016/0016-5085(87)90016-3. [DOI] [PubMed] [Google Scholar]
  19. Tsai B. S., Kessler L. K., Schoenhard G., Collins P. W., Bauer R. F. E-type prostaglandin binding sites in isolated canine parietal cells: elucidation with (3H) misoprostol free acid. Z Gastroenterol. 1987 Apr;25(4):201–206. [PubMed] [Google Scholar]
  20. Vischer P., Casals-Stenzel J. Pharmacological properties of Ciloprost, a stable prostacyclin analogue, on the gastrointestinal tract. Prostaglandins Leukot Med. 1982 Nov;9(5):517–529. doi: 10.1016/0262-1746(82)90033-6. [DOI] [PubMed] [Google Scholar]
  21. Wollin A. Carbonic anhydrase activity and aminopyrine uptake in isolated gastric mucosal cells. Am J Physiol. 1984 Sep;247(3 Pt 1):G213–G219. doi: 10.1152/ajpgi.1984.247.3.G213. [DOI] [PubMed] [Google Scholar]
  22. Wollin A., Soll A. H., Samloff I. M. Actions of histamine, secretin, and PGE2 on cyclic AMP production by isolated canine fundic mucosal cells. Am J Physiol. 1979 Nov;237(5):E437–E443. doi: 10.1152/ajpendo.1979.237.5.E437. [DOI] [PubMed] [Google Scholar]
  23. Woo S. K., Roszkowski P., Waterbury L. D., Garay G. L. Gastric mucosal binding studies with enprostil: a potent anti-ulcer prostaglandin. Prostaglandins. 1986 Aug;32(2):243–257. doi: 10.1016/0090-6980(86)90129-2. [DOI] [PubMed] [Google Scholar]

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