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. 1988 Nov;405:219–232. doi: 10.1113/jphysiol.1988.sp017330

Membrane properties of primary sensory neurones of the cat after peripheral reinnervation.

C Belmonte 1, R Gallego 1, A Morales 1
PMCID: PMC1190973  PMID: 3255791

Abstract

1. The properties of sensory neurones in the petrosal ganglion of the cat were examined in vitro with intracellular electrodes 2-30 days after cutting the carotid or the glossopharyngeal nerve or 30-120 days after crushing the nerves and allowing time for peripheral reinnervation. Both glossopharyngeal and carotid neurones with conduction velocities faster than 2 m/s and humps on the falling phase of their action potentials were studied. 2. Two days after axotomy, the action potential of glossopharyngeal cells showed increased maximum rate of depolarization and decreased after-hyperpolarization (AHP) amplitude. These cells also showed a decrease in time-dependent inward rectification and conduction velocity. No significant changes were observed in carotid neurones at this stage. 3. Four days after axotomy, these changes were still present in glossopharyngeal cells and the duration of the AHP had also decreased. Among carotid neurones there was an increase in spike duration and a decrease in time-dependent rectification and in the amplitude and duration of the AHP. 4. Eight days after axotomy glossopharyngeal cells additionally showed an increased spike duration and decreased rheobase, while carotid neurones had also decreased in conduction velocity. 5. All the changes described were still present 16 days after cutting the nerves. In addition, the input resistance increased in both types of cells. 6. The effects of axotomy persisted 30 days after cutting the nerve, except for the AHP amplitude in both types of cells and the spike duration in glossopharyngeal neurones which had recovered to near control values. 7. Thirty days after crushing the carotid nerve, some carotid neurones showed chemosensory activity, indicating that reinnervation of the carotid body had taken place. In these cells, the properties altered by axotomy had recovered except for the conduction velocity. 8. Four months after crushing the glossopharyngeal nerve, activity evoked by mechanical and chemical stimulation of the tongue could be recorded from the nerve, indicating that sensory reinnervation had taken place. The electrical properties of the glossopharyngeal cells were normal except for conduction velocity which was still slower than the control value. 9. It is concluded that the electrical membrane properties altered by axotomy revert to normal after peripheral sensory regeneration.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aldskogius H., Arvidsson J., Grant G. The reaction of primary sensory neurons to peripheral nerve injury with particular emphasis on transganglionic changes. Brain Res. 1985 Sep;357(1):27–46. doi: 10.1016/0165-0173(85)90006-2. [DOI] [PubMed] [Google Scholar]
  2. Belmonte C., Gallego R. Membrane properties of cat sensory neurones with chemoreceptor and baroreceptor endings. J Physiol. 1983 Sep;342:603–614. doi: 10.1113/jphysiol.1983.sp014871. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Czéh G., Kudo N., Kuno M. Membrane properties and conduction velocity in sensory neurones following central or peripheral axotomy. J Physiol. 1977 Aug;270(1):165–180. doi: 10.1113/jphysiol.1977.sp011944. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Devor M., Govrin-Lippmann R. Selective regeneration of sensory fibers following nerve crush injury. Exp Neurol. 1979 Aug;65(2):243–254. doi: 10.1016/0014-4886(79)90094-3. [DOI] [PubMed] [Google Scholar]
  5. Dziegielewska K. M., Evans C. A., Saunders N. R. Rapid effect of nerve injury upon axonal transport of phospholipids. J Physiol. 1980 Jul;304:83–98. doi: 10.1113/jphysiol.1980.sp013311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. ECCLES J. C., ECCLES R. M., SHEALY C. N. An investigation into the effect of degenerating primary afferent fibers on the monosynpatic innervation of motoneurons. J Neurophysiol. 1962 Jul;25:544–558. doi: 10.1152/jn.1962.25.4.544. [DOI] [PubMed] [Google Scholar]
  7. ECCLES J. C., KRNJEVIC K., MILEDI R. Delayed effects of peripheral severance of afferent nerve fibres on the efficacy of their central synapses. J Physiol. 1959 Jan 28;145(1):204–220. doi: 10.1113/jphysiol.1959.sp006136. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gallego R., Ivorra I., Morales A. Effects of central or peripheral axotomy on membrane properties of sensory neurones in the petrosal ganglion of the cat. J Physiol. 1987 Oct;391:39–56. doi: 10.1113/jphysiol.1987.sp016724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gallego R., Kuno M., Núez R., Snider W. D. Disuse enhances synaptic efficacy in spinal mononeurones. J Physiol. 1979 Jun;291:191–205. doi: 10.1113/jphysiol.1979.sp012807. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gallego R., Kuno M., Núez R., Snider W. D. Enhancement of synaptic function in cat motoneurones during peripheral sensory regeneration. J Physiol. 1980 Sep;306:205–218. doi: 10.1113/jphysiol.1980.sp013392. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Goldring J. M., Kuno M., Núez R., Snider W. D. Reaction of synapses on motoneurones to section and restoration of peripheral sensory connexions in the cat. J Physiol. 1980 Dec;309:185–198. doi: 10.1113/jphysiol.1980.sp013503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gordon T., Kelly M. E., Sanders E. J., Shapiro J., Smith P. A. The effects of axotomy on bullfrog sympathetic neurones. J Physiol. 1987 Nov;392:213–229. doi: 10.1113/jphysiol.1987.sp016777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Horch K. W., Lisney S. J. On the number and nature of regenerating myelinated axons after lesions of cutaneous nerves in the cat. J Physiol. 1981;313:275–286. doi: 10.1113/jphysiol.1981.sp013664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kelly M. E., Gordon T., Shapiro J., Smith P. A. Axotomy affects calcium-sensitive potassium conductance in sympathetic neurones. Neurosci Lett. 1986 Jun 18;67(2):163–168. doi: 10.1016/0304-3940(86)90391-5. [DOI] [PubMed] [Google Scholar]
  15. Kuno M., Miyata Y., Muñoz-Martinez E. J. Differential reaction of fast and slow alpha-motoneurones to axotomy. J Physiol. 1974 Aug;240(3):725–739. doi: 10.1113/jphysiol.1974.sp010631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kuno M., Miyata Y., Muñoz-Martinez E. J. Properties of fast and slow alpha motoneurones following motor reinnervation. J Physiol. 1974 Oct;242(1):273–288. doi: 10.1113/jphysiol.1974.sp010706. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Morales A., Ivorra I., Gallego R. Membrane properties of glossopharyngeal sensory neurons in the petrosal ganglion of the cat. Brain Res. 1987 Jan 20;401(2):340–346. doi: 10.1016/0006-8993(87)91418-1. [DOI] [PubMed] [Google Scholar]
  18. Ochs S. Trophic functions of the neuron. 3. Mechanisms of neurotrophic interactions. Systems of material transport in nerve fibers (axoplasmic transport) related to nerve function and trophic control. Ann N Y Acad Sci. 1974 Mar 22;228(0):202–223. doi: 10.1111/j.1749-6632.1974.tb20511.x. [DOI] [PubMed] [Google Scholar]
  19. Zapata P., Stensaas L. J., Eyzaguirre C. Axon regeneration following a lesion of the carotid nerve: electrophysiological and ultrastructural observations. Brain Res. 1976 Aug 27;113(2):235–253. doi: 10.1016/0006-8993(76)90939-2. [DOI] [PubMed] [Google Scholar]

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