Skip to main content
PMC home page
Annals of Surgery logoLink to Annals of Surgery
. 1998 Mar;227(3):365–371. doi: 10.1097/00000658-199803000-00008

Prognostic value of platelet-derived growth factor-A (PDGF-A) in gastric carcinoma.

M Katano 1, M Nakamura 1, K Fujimoto 1, K Miyazaki 1, T Morisaki 1
PMCID: PMC1191274  PMID: 9527059

Abstract

OBJECTIVE: Because our previous study indicated that PDGF-A mRNA expression in biopsy specimens might identify a subgroup of high-risk patients with gastric carcinoma, in this study we analyzed the prognostic value of platelet-derived growth factor-A (PDGF-A) gene expression in gastric carcinoma biopsy specimens. METHODS: Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to analyze the PDGF-A gene expression in 65 gastric carcinoma endoscopic biopsy specimens. The 65 patients were divided into a PDGF-A-positive group (29 patients) and a PDGF-A-negative group (36 patients). RESULTS: On the basis of 2-year follow-up data, the PDGF-A-positive group demonstrated a shorter overall survival rate compared with the PDGF-A-negative group (p < 0.0001). A similar correlation was found in 34 advanced-stage patients (p = 0.003) and in 24 advanced-stage patients who underwent a curative resection (p = 0.003). Multivariance analysis indicated that the transcription of PDGF-A gene is a potent prognostic factor that is independent of the traditional pathologic parameters. CONCLUSIONS: Expression of PDGF-A mRNA in gastric biopsy specimens may be a new preoperative prognostic parameter in gastric carcinoma.

Full text

PDF
365

Images in this article

367Figure 1.
on p.367
370Figure 5.
on p.370

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albino A. P., Davis B. M., Nanus D. M. Induction of growth factor RNA expression in human malignant melanoma: markers of transformation. Cancer Res. 1991 Sep 15;51(18):4815–4820. [PubMed] [Google Scholar]
  2. Ariad S., Seymour L., Bezwoda W. R. Platelet-derived growth factor (PDGF) in plasma of breast cancer patients: correlation with stage and rate of progression. Breast Cancer Res Treat. 1991 Dec;20(1):11–17. doi: 10.1007/BF01833352. [DOI] [PubMed] [Google Scholar]
  3. Betsholtz C., Johnsson A., Heldin C. H., Westermark B., Lind P., Urdea M. S., Eddy R., Shows T. B., Philpott K., Mellor A. L. cDNA sequence and chromosomal localization of human platelet-derived growth factor A-chain and its expression in tumour cell lines. Nature. 1986 Apr 24;320(6064):695–699. doi: 10.1038/320695a0. [DOI] [PubMed] [Google Scholar]
  4. Bronzert D. A., Pantazis P., Antoniades H. N., Kasid A., Davidson N., Dickson R. B., Lippman M. E. Synthesis and secretion of platelet-derived growth factor by human breast cancer cell lines. Proc Natl Acad Sci U S A. 1987 Aug;84(16):5763–5767. doi: 10.1073/pnas.84.16.5763. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  6. Cockett M. I., Birch M. L., Murphy G., Hart I. R., Docherty A. J. Metalloproteinase domain structure, cellular invasion and metastasis. Biochem Soc Trans. 1994 Feb;22(1):55–57. doi: 10.1042/bst0220055. [DOI] [PubMed] [Google Scholar]
  7. Deuel T. F., Huang J. S. Platelet-derived growth factor. Structure, function, and roles in normal and transformed cells. J Clin Invest. 1984 Sep;74(3):669–676. doi: 10.1172/JCI111482. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Friess H., Yamanaka Y., Büchler M., Ebert M., Beger H. G., Gold L. I., Korc M. Enhanced expression of transforming growth factor beta isoforms in pancreatic cancer correlates with decreased survival. Gastroenterology. 1993 Dec;105(6):1846–1856. doi: 10.1016/0016-5085(93)91084-u. [DOI] [PubMed] [Google Scholar]
  9. Hart I. R., Saini A. Biology of tumour metastasis. Lancet. 1992 Jun 13;339(8807):1453–1457. doi: 10.1016/0140-6736(92)92039-i. [DOI] [PubMed] [Google Scholar]
  10. Heldin C. H., Westermark B. Growth factors: mechanism of action and relation to oncogenes. Cell. 1984 May;37(1):9–20. doi: 10.1016/0092-8674(84)90296-4. [DOI] [PubMed] [Google Scholar]
  11. Henriksen R., Funa K., Wilander E., Bäckström T., Ridderheim M., Oberg K. Expression and prognostic significance of platelet-derived growth factor and its receptors in epithelial ovarian neoplasms. Cancer Res. 1993 Oct 1;53(19):4550–4554. [PubMed] [Google Scholar]
  12. Hermanson M., Funa K., Hartman M., Claesson-Welsh L., Heldin C. H., Westermark B., Nistér M. Platelet-derived growth factor and its receptors in human glioma tissue: expression of messenger RNA and protein suggests the presence of autocrine and paracrine loops. Cancer Res. 1992 Jun 1;52(11):3213–3219. [PubMed] [Google Scholar]
  13. Hinds P. W., Dowdy S. F., Eaton E. N., Arnold A., Weinberg R. A. Function of a human cyclin gene as an oncogene. Proc Natl Acad Sci U S A. 1994 Jan 18;91(2):709–713. doi: 10.1073/pnas.91.2.709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ishikawa F., Miyazono K., Hellman U., Drexler H., Wernstedt C., Hagiwara K., Usuki K., Takaku F., Risau W., Heldin C. H. Identification of angiogenic activity and the cloning and expression of platelet-derived endothelial cell growth factor. Nature. 1989 Apr 13;338(6216):557–562. doi: 10.1038/338557a0. [DOI] [PubMed] [Google Scholar]
  15. Jatzko G. R., Lisborg P. H., Denk H., Klimpfinger M., Stettner H. M. A 10-year experience with Japanese-type radical lymph node dissection for gastric cancer outside of Japan. Cancer. 1995 Oct 15;76(8):1302–1312. doi: 10.1002/1097-0142(19951015)76:8<1302::aid-cncr2820760803>3.0.co;2-8. [DOI] [PubMed] [Google Scholar]
  16. Kim K. J., Li B., Winer J., Armanini M., Gillett N., Phillips H. S., Ferrara N. Inhibition of vascular endothelial growth factor-induced angiogenesis suppresses tumour growth in vivo. Nature. 1993 Apr 29;362(6423):841–844. doi: 10.1038/362841a0. [DOI] [PubMed] [Google Scholar]
  17. Krüger-Krasagakes S., Krasagakis K., Garbe C., Schmitt E., Hüls C., Blankenstein T., Diamantstein T. Expression of interleukin 10 in human melanoma. Br J Cancer. 1994 Dec;70(6):1182–1185. doi: 10.1038/bjc.1994.469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Maeda K., Chung Y. S., Ogawa Y., Takatsuka S., Kang S. M., Ogawa M., Sawada T., Sowa M. Prognostic value of vascular endothelial growth factor expression in gastric carcinoma. Cancer. 1996 Mar 1;77(5):858–863. doi: 10.1002/(sici)1097-0142(19960301)77:5<858::aid-cncr8>3.0.co;2-a. [DOI] [PubMed] [Google Scholar]
  19. Maehara Y., Okuyama T., Moriguchi S., Orita H., Kusumoto H., Korenaga D., Sugimachi K. Prophylactic lymph node dissection in patients with advanced gastric cancer promotes increased survival time. Cancer. 1992 Jul 15;70(2):392–395. doi: 10.1002/1097-0142(19920715)70:2<392::aid-cncr2820700204>3.0.co;2-p. [DOI] [PubMed] [Google Scholar]
  20. Majesky M. W., Reidy M. A., Bowen-Pope D. F., Hart C. E., Wilcox J. N., Schwartz S. M. PDGF ligand and receptor gene expression during repair of arterial injury. J Cell Biol. 1990 Nov;111(5 Pt 1):2149–2158. doi: 10.1083/jcb.111.5.2149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Maruyama K., Gunvén P., Okabayashi K., Sasako M., Kinoshita T. Lymph node metastases of gastric cancer. General pattern in 1931 patients. Ann Surg. 1989 Nov;210(5):596–602. doi: 10.1097/00000658-198911000-00005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Miwa K. Evaluation of the TNM classification of stomach cancer and proposal for its rational stage-grouping. Jpn J Clin Oncol. 1984 Sep;14(3):385–410. [PubMed] [Google Scholar]
  23. Nakamura M., Katano M., Fujimoto K., Morisaki T. A new prognostic strategy for gastric carcinoma: mRNA expression of tumor growth-related factors in endoscopic biopsy specimens. Ann Surg. 1997 Jul;226(1):35–42. doi: 10.1097/00000658-199707000-00005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Richardson W. D., Pringle N., Mosley M. J., Westermark B., Dubois-Dalcq M. A role for platelet-derived growth factor in normal gliogenesis in the central nervous system. Cell. 1988 Apr 22;53(2):309–319. doi: 10.1016/0092-8674(88)90392-3. [DOI] [PubMed] [Google Scholar]
  25. Safi A., Sadmi M., Martinet N., Menard O., Vaillant P., Gallati H., Hosang M., Martinet Y. Presence of elevated levels of platelet-derived growth factor (PDGF) in lung adenocarcinoma pleural effusions. Chest. 1992 Jul;102(1):204–207. doi: 10.1378/chest.102.1.204. [DOI] [PubMed] [Google Scholar]
  26. Silver B. J. Platelet-derived growth factor in human malignancy. Biofactors. 1992 Apr;3(4):217–227. [PubMed] [Google Scholar]
  27. Smits A., Funa K., Vassbotn F. S., Beausang-Linder M., af Ekenstam F., Heldin C. H., Westermark B., Nistér M. Expression of platelet-derived growth factor and its receptors in proliferative disorders of fibroblastic origin. Am J Pathol. 1992 Mar;140(3):639–648. [PMC free article] [PubMed] [Google Scholar]
  28. Söderdahl G., Betsholtz C., Johansson A., Nilsson K., Bergh J. Differential expression of platelet-derived growth factor and transforming growth factor genes in small- and non-small-cell human lung carcinoma lines. Int J Cancer. 1988 Apr 15;41(4):636–641. doi: 10.1002/ijc.2910410426. [DOI] [PubMed] [Google Scholar]
  29. Tahara E., Sumiyoshi H., Hata J., Yasui W., Taniyama K., Hayashi T., Nagae S., Sakamoto S. Human epidermal growth factor in gastric carcinoma as a biologic marker of high malignancy. Jpn J Cancer Res. 1986 Feb;77(2):145–152. [PubMed] [Google Scholar]
  30. Takanami I., Imamura T., Yamamoto Y., Kodaira S. Usefulness of platelet-derived growth factor as a prognostic factor in pulmonary adenocarcinoma. J Surg Oncol. 1995 Jan;58(1):40–43. doi: 10.1002/jso.2930580109. [DOI] [PubMed] [Google Scholar]
  31. Wang T. C., Cardiff R. D., Zukerberg L., Lees E., Arnold A., Schmidt E. V. Mammary hyperplasia and carcinoma in MMTV-cyclin D1 transgenic mice. Nature. 1994 Jun 23;369(6482):669–671. doi: 10.1038/369669a0. [DOI] [PubMed] [Google Scholar]

Articles from Annals of Surgery are provided here courtesy of Lippincott, Williams, and Wilkins

RESOURCES