Skip to main content
NCBI home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1988 Jun;400:223–236. doi: 10.1113/jphysiol.1988.sp017118

Evidence for co-transport of sodium, potassium and chloride in mouse pancreatic islets.

P Lindström 1, L Norlund 1, P E Sandstöm 1, J Sehlin 1
PMCID: PMC1191805  PMID: 3047367

Abstract

1. The presence of a loop diuretic-sensitive co-transport system for Na+, K+ and Cl- was tested in isolated pancreatic islets. 2. Substitution of Cl- with the impermeant anion isethionate or addition of frusemide both reduced the ouabain-resistant islets uptake of 86Rb+ (K+ marker) without affecting the ouabain-sensitive uptake or equilibrium content of 86Rb+. The effects of Cl- substitution and frusemide were overlapping. 3. D-Glucose reduced the ouabain-resistant islets uptake of 86Rb+. This effect was additive to the effect of Cl- substitution or frusemide. 4. Substitution of Cl- with isethionate or addition of frusemide both reduced the efflux of 86Rb+ from the islets. These effects were additive to the reduction of 86Rb+ efflux induced by D-glucose. 5. Substitution of K+ or Na+ with choline reduced the equilibrium content of 36Cl- in the pancreatic islets. 6. These data are compatible with the operation in the pancreatic beta-cells of a loop diuretic-sensitive co-transport system for Na+, K+ and Cl-, that may serve as an inwardly directed Cl- pump.

Full text

PDF
223

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boschero A. C., Malaisse W. J. Stimulus-secretion coupling of glucose-induced insulin release. XXIX. Regulation of 86Rb+ efflux from perfused islets. Am J Physiol. 1979 Feb;236(2):E139–E146. doi: 10.1152/ajpendo.1979.236.2.E139. [DOI] [PubMed] [Google Scholar]
  2. Dean P. M., Matthews E. K. Glucose-induced electrical activity in pancreatic islet cells. J Physiol. 1970 Sep;210(2):255–264. doi: 10.1113/jphysiol.1970.sp009207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Freinkel N., Younsi C. E., Bonnar J., Dawson R. M. Rapid transient efflux of phosphate ions from pancreatic islets as an early action of insulin secretagogues. J Clin Invest. 1974 Nov;54(5):1179–1189. doi: 10.1172/JCI107861. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Frizzell R. A., Field M., Schultz S. G. Sodium-coupled chloride transport by epithelial tissues. Am J Physiol. 1979 Jan;236(1):F1–F8. doi: 10.1152/ajprenal.1979.236.1.F1. [DOI] [PubMed] [Google Scholar]
  5. Giebisch G. The use of a diuretic agent as a probe to investigate site and mechanism of ion transport processes. Arzneimittelforschung. 1985;35(1A):336–342. [PubMed] [Google Scholar]
  6. Greger R., Schlatter E. Properties of the basolateral membrane of the cortical thick ascending limb of Henle's loop of rabbit kidney. A model for secondary active chloride transport. Pflugers Arch. 1983 Mar;396(4):325–334. doi: 10.1007/BF01063938. [DOI] [PubMed] [Google Scholar]
  7. Hahn H. J., Hellman B., Lernmark A., Sehlin J., Täljedal I. B. The pancreatic beta-cell recognition of insulin secretogogues. Influence of neuraminidase treatment on the release of insulin and the islet content of insulin, sialic acid, and cyclic adenosine 3':5'-monophosphate. J Biol Chem. 1974 Aug 25;249(16):5275–5284. [PubMed] [Google Scholar]
  8. Hellman B., Idahl L. A., Lernmark A., Sehlin J., Täljedal I. B. The pancreatic beta-cell recognition of insulin secretagogues. Effects of calcium and sodium on glucose metabolism and insulin release. Biochem J. 1974 Jan;138(1):33–45. doi: 10.1042/bj1380033. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hellman B., Sehlin J., Täljedal I. B. Transport of -aminoisobutyric acid in mammalian pancretic -cells. Diabetologia. 1971 Aug;7(4):256–265. doi: 10.1007/BF01211878. [DOI] [PubMed] [Google Scholar]
  10. Hellman B. Studies in obese-hyperglycemic mice. Ann N Y Acad Sci. 1965 Oct 8;131(1):541–558. doi: 10.1111/j.1749-6632.1965.tb34819.x. [DOI] [PubMed] [Google Scholar]
  11. Henquin J. C. D-glucose inhibits potassium efflux from pancreatic islet cells. Nature. 1978 Jan 19;271(5642):271–273. doi: 10.1038/271271a0. [DOI] [PubMed] [Google Scholar]
  12. Henquin J. C., Lambert A. E. Bicarbonate modulation of glucose-9nduced biphasic insulin release by rat islets. Am J Physiol. 1976 Sep;231(3):713–721. doi: 10.1152/ajplegacy.1976.231.3.713. [DOI] [PubMed] [Google Scholar]
  13. Henquin J. C. The potassium permeability of pancreatic islet cells: mechanisms of control and influence on insulin release. Horm Metab Res Suppl. 1980;Suppl 10:66–73. [PubMed] [Google Scholar]
  14. Howell S. L., Taylor K. W. Potassium ions and the secretion of insulin by islets of Langerhans incubated in vitro. Biochem J. 1968 Jun;108(1):17–24. doi: 10.1042/bj1080017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Idahl L. A., Lernmark A., Sehlin J., Täljedal I. B. Studies on the function of pancreatic islet cell membranes. J Physiol (Paris) 1976 Nov;72(6):729–746. [PubMed] [Google Scholar]
  16. Lindström P., Norlund L., Sehlin J. Glucose reduces both Rb+ influx and efflux in pancreatic islet cells. FEBS Lett. 1986 May 5;200(1):67–70. doi: 10.1016/0014-5793(86)80512-9. [DOI] [PubMed] [Google Scholar]
  17. Lindström P., Norlund L., Sehlin J. Potassium and chloride fluxes are involved in volume regulation in mouse pancreatic islet cells. Acta Physiol Scand. 1986 Dec;128(4):541–546. doi: 10.1111/j.1748-1716.1986.tb08010.x. [DOI] [PubMed] [Google Scholar]
  18. Lindström P., Sehlin J. 5-hydroxytryptamine stimulates 86Rb+ efflux from pancreatic beta-cells. Biochim Biophys Acta. 1982 Jul 22;720(4):400–404. doi: 10.1016/0167-4889(82)90118-5. [DOI] [PubMed] [Google Scholar]
  19. Meissner H. P., Preissler M. Ionic mechanisms of the glucose-induced membrane potential changes in B-cells. Horm Metab Res Suppl. 1980;Suppl 10:91–99. [PubMed] [Google Scholar]
  20. Norlund L., Sehlin J. Effect of glibenclamide on the osmotic resistance of pancreatic beta-cells. Acta Physiol Scand. 1984 Mar;120(3):407–415. doi: 10.1111/j.1748-1716.1984.tb07401.x. [DOI] [PubMed] [Google Scholar]
  21. Pace C. S., Smith J. S. The role of chemiosmotic lysis in the exocytotic release of insulin. Endocrinology. 1983 Sep;113(3):964–969. doi: 10.1210/endo-113-3-964. [DOI] [PubMed] [Google Scholar]
  22. Saier M. H., Jr, Boyden D. A. Mechanism, regulation and physiological significance of the loop diuretic-sensitive NaCl/KCl symport system in animal cells. Mol Cell Biochem. 1984;59(1-2):11–32. doi: 10.1007/BF00231303. [DOI] [PubMed] [Google Scholar]
  23. Sandström P. E., Sehlin J. Stereoselective inhibition of chloride transport by loop diuretics in pancreatic beta-cells. Eur J Pharmacol. 1987 Dec 15;144(3):389–392. doi: 10.1016/0014-2999(87)90394-3. [DOI] [PubMed] [Google Scholar]
  24. Sehlin J. Are Cl- mechanisms in mouse pancreatic islets involved in insulin release? Ups J Med Sci. 1981;86(2):177–182. doi: 10.3109/03009738109179226. [DOI] [PubMed] [Google Scholar]
  25. Sehlin J. Evidence for voltage-dependent C1- permeability in mouse pancreatic beta-cells. Biosci Rep. 1987 Jan;7(1):67–72. doi: 10.1007/BF01122729. [DOI] [PubMed] [Google Scholar]
  26. Sehlin J. Interrelationship between chloride fluxes in pancreatic islets and insulin release. Am J Physiol. 1978 Nov;235(5):E501–E508. doi: 10.1152/ajpendo.1978.235.5.E501. [DOI] [PubMed] [Google Scholar]
  27. Sehlin J., Meissner H. P. Effects of Cl- deficiency on the membrane potential in mouse pancreatic beta-cells. Biochim Biophys Acta. 1988 Jan 22;937(2):309–318. doi: 10.1016/0005-2736(88)90253-2. [DOI] [PubMed] [Google Scholar]
  28. Sehlin J., Taljedal I. B. Glucose-induced decrease in Rb+ permeability in pancreatic beta cells. Nature. 1975 Feb 20;253(5493):635–636. doi: 10.1038/253635a0. [DOI] [PubMed] [Google Scholar]
  29. Sehlin J., Täljedal I. B. Transport of rubidium and sodium in pancreatic islets. J Physiol. 1974 Oct;242(2):505–515. doi: 10.1113/jphysiol.1974.sp010720. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Sehlin J. Univalent ions in islet cell function. Horm Metab Res Suppl. 1980;Suppl 10:73–80. [PubMed] [Google Scholar]
  31. Somers G., Sener A., Devis G., Malaisse W. J. The stimulus-secretion coupling of glucose-induced insulin release. XLV. The anion-osmotic hypothesis for exocytosis. Pflugers Arch. 1980 Dec;388(3):249–253. doi: 10.1007/BF00658490. [DOI] [PubMed] [Google Scholar]
  32. Stauffacher W., Lambert A. E., Vecchio D., Renold A. E. Measurements of insulin activities in pancreas and serum of mice with spontaneous ("Obese" and "New Zealand Obese") and induced (Goldthioglucose) obesity and hyperglycemia, with considerations on the pathogenesis of the spontaneous syndrome. Diabetologia. 1967 Apr;3(2):230–237. doi: 10.1007/BF01222200. [DOI] [PubMed] [Google Scholar]
  33. Stauffacher W., Renold A. E. Effect of insulin in vivo on diaphragm and adipose tissue of obese mice. Am J Physiol. 1969 Jan;216(1):98–105. doi: 10.1152/ajplegacy.1969.216.1.98. [DOI] [PubMed] [Google Scholar]
  34. Suzuki K., Petersen O. H. The effect of Na+ and Cl- removal and of loop diuretics on acetylcholine-evoked membrane potential changes in mouse lacrimal acinar cells. Q J Exp Physiol. 1985 Jul;70(3):437–445. doi: 10.1113/expphysiol.1985.sp002927. [DOI] [PubMed] [Google Scholar]
  35. Tamagawa T., Henquin J. C. Chloride modulation of insulin release, 86Rb+ efflux, and 45Ca2+ fluxes in rat islets stimulated by various secretagogues. Diabetes. 1983 May;32(5):416–423. doi: 10.2337/diab.32.5.416. [DOI] [PubMed] [Google Scholar]
  36. Wollheim C. B., Sharp G. W. Regulation of insulin release by calcium. Physiol Rev. 1981 Oct;61(4):914–973. doi: 10.1152/physrev.1981.61.4.914. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES